Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 55 for “"Cryo-electron microscopy (cryo-EM)"”.
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A Graph-Based Algorithm to Determine Protein Structure from Cryo-EM Data
Cryo-electron microscopy: cryo-EM) provides 3D density maps of proteins, but these maps do not have sufficiently high resolution to directly yield atomic-scale models. Previous work has shown that features known as secondary structures can be located in these density maps. A second source of …
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Deep Learning for Segmentation Of 3D Cryo-EM Images
<p>Cryo-electron microscopy (cryo-EM) is an emerging biophysical technique for structural determination of protein complexes. However, accurate detection of secondary structures is still challenging when cryo-EM density maps are at medium resolutions (5-10 Å). Most existing methods are image …
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Investigating the mechanisms of protein synthesis using multi-resolution structural data
… culminated with the 2009 Nobel Prize in Chemistry. Concomitantly, advances in cryo-electron microscopy (cryo-EM) enabled the determination of images of the ribosome trapped in functional states at ever increasing resolution. In order to study different aspects of ribosome function at the …
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Mechanisms of Translational Control
This work employs structural and biophysical approaches to investigate the fundamental mechanisms of translational control in ribosomes. Specifically, we examine the molecular basis of Sec stop codon suppression and insertion, as well as the phenotypic consequences of perturbations to this process. …
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Machine Learning for Reconstructing Dynamic Protein Structures from Cryo-EM Images
… of dynamic biomolecular complexes via cryo-electron microscopy (cryo-EM). In single particle cryo-EM, the central problem is to reconstruct the 3D structure of a target biomolecular complex from a set of noisy and randomly oriented 2D projection images, a challenging inverse problem …
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Simulating, refining and modeling protein structures with multi-scale methods
… biological method such as Fiber Diffraction, cryo-Electron Microscopy (cryo-EM), Small Angle X-ray Scattering (SAXS) and X-ray Crystallography, we show that our methods can be employed to improve the study of protein structures. In detail, the atomic structure of actin filament was refined …
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Interactive Analysis of Protein Structure and Dynamics
… unfamiliar techniques on their biological systems of interest without the need to download software or databases. To facilitate usage, I included visualization allowing intuitive understanding and analysis. We developed the NGL molecular web-viewer, which accesses hardware-accelerated graphics …
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Trpv5: Structure, Function And Drug Discovery
… calcium selective ion channel that regulates systemic calcium homeostasis by acting as a critical gate for calcium reabsorption in the kidney. Human polymorphisms of this channel have exemplified the importance of TRPV5 in disorders of calcium homeostasis, but atomic level information regarding …
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Structural studies of trans-translation
… for the survival of the cell. Ribosomes themselves are made up of both RNA and protein, and are composed of two subunits, each with a separate function. The small subunit reads the mRNA message, directing the large subunit to synthesize a sequence of amino acids to form a protein. In many …
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Structure and Function of the ER-Membrane Complex
The ER-Membrane Complex (EMC) is a highly conserved heterodecameric membrane protein complex, found in the Endoplasmic Reticulum (ER) membrane. The EMC has been implicated in a wide range of processes including viral reproduction, inter-organelle phospholipid transfer, cholesterol biosynthesis and …
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Structural studies of the mitochondrial ribosome and co-translational insertion of membrane proteins
… has retained its own translational system to include mitochondrial ribosomes (mitoribosome) to translate these proteins along with other proteins which is species dependent. The mitoribosome is also involved in co-translational insertion of proteins destined for the inner mitochondrial …
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TOWARD STRUCTURAL INSIGHTS INTO THE RECOGNITION OF H3K27 TRIMETHYLATION BY CANONICAL PRC1
… of key developmental genes involved in embryogenesis, pluripotency and tumorigenesis. Alterations in their activity are hallmarks in developmental disorders and cancer as chromatin modifiers are one of the most frequently mutated groups of genes in human tumors. Polycomb Repressive …
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Understanding Recognition of Primary MicroRNAs by Human Drosha/DGCR8
… the initiation of microRNA biogenesis. Past biochemical and structural studies have left numerous unanswered questions about pri-miRNA processing. Using an in vitro system with human Drosha and DGCR8, I pinpointed the role of heme binding in DGCR8-mediated regulation of pri-miRNA processing, and …
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Affinity cryo-electron microscopy: Methods development and applications
<p>Single particle cryo-electron microscopy (cryo-EM) is an emerging powerful tool for structural studies of macromolecular assemblies. Although less concentrated and smaller amounts of samples are required for single particle cryo-EM compared to X-ray crystallography, it remains challenging to …
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Helical Reconstruction in RELION
Helical assemblies of proteins are ubiquitous in nature and they perform vital functions in a wide range of organisms. The recent development of direct electron detectors and other imaging techniques in cryo-electron microscopy (cryo-EM) has opened new possibilities in solving helical structures at …
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STRUCTURAL INSIGHTS INTO RNA POLYMERASE III PRE-INITIATION COMPLEX AND THE PIONEER ROLE OF OCT1
… is selectively recruited to snRNA promoters remain poorly understood. This is particularly notable because Pol II- and Pol III-transcribed snRNA promoters share highly similar architectures and rely on the same core transcription factor, SNAPc. How a single complex enables mutually exclusive …
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Actin-like filament A (AlfA) and AlfA-driven plasmid segregation
… upon nucleotide binding and their involvement in various cellular processes including cell shape determination, cell division, and DNA segregation led to a reconsideration of the evolutionary history of life. Actin-like filament A (AlfA) is a bacterial actin involved in plasmid …
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Unraveling the Functions of Synaptotagmin and Munc13 in Neurotransmitter Release
… that brings synaptic vesicles and plasma membrane together. This complex is disassembled by NSF/α-SNAP (soluble N-ethylmaleimide sensitive fusion protein/NSF attachment protein). Munc18 binds to syntaxin, which keeps syntaxin in its closed confirmation and prevents to form the SNARE …
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The rational structure-based design of a protein nanoparticle presenting a chimeric MenB antigen
… is a leading cause of meningitis and septicaemia. Vaccines rank amongst the most important public health interventions for combating these diseases. As vaccine development orientates towards more minimalist subunit antigen-based vaccines, new adjuvant systems are increasingly needed to boost …
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MOLECULAR MECHANISMS OF ACTIN FILAMENT ASSEMBLY AND TURNOVER
… essential cellular functions. This versatility stems primarily from the myriads of actin binding proteins (ABPs) that bind to and regulate actin. A substantial proportion of these proteins modify actin turnover at filament ends, defined as the flux between actin’s monomeric G-actin state and its …
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