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Showing 1 to 20 of 54 for “"Crude extract"”.
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Antioxidant Activity of Ampelopsis Grossedentata Crude Extract and its Major Component Dihydromyricetin
… antioxidants. Antioxidant activity of Teng Cha extract and its major component dihydromyricetin has been reported, but no studies have provided clear evidence for the antioxidant effectiveness of Tech Cha extracts. The goal of this study was to measure the antioxidant activity of Teng Cha …
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Antibacterial activity of the crude extract and fractions of spirostachys africana against multi-drug resistant bacteria
… of bacteria to antibiotics, S. africana extracts can provide an opportunity to finding new antibacterial compounds that can be used as the foundation for formulating new antimicrobial drugs. Objectives: The aim of this study was to screen antibacterial activity of the crude extract and …
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Antioxidant activity and phytochemical evaluations of selected medicinal plants
The methanolic crude extract of aerial parts of the plant Scrophularia nodosa was shown to have potent DPPH radical scavenging activity (IC50 = 48.75 ± 7.00 μg/ml). Activity-guided fractionation resulted in the isolation of three principal antioxidant compounds; acteoside, angoroside C and …
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Phytochemical and pharmacological studies on some endemic Yucatecan medicinal plants
… stem/bank and leaves of each plant species were extracted using ethanol and the resulting crude extracts were tested for their cytotoxic effect using the modified MTT (3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) assay followed by a bioassay-guided fractionation of the most …
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Antiviral potential of hadjod (Cissus quadrangularis) extract against newcastle disease virus.
… impact on the poultry industry. Ethanol (EtOH) crude extract from the stem of Cissus quadrangularis (CQL) was prepared and tested against Newcastle Disease Virus (NDV) replication using an in- ovo assay in embryonic chickens. Cissus quadrangularis L, is a perennial plant of the Vitaceae family, …
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Pilot-scale process development for the purification of the recombinant antibody 2G12 from transgenic tobacco
… equipment: manual harvest, dispersion and extraction, pH shift in the crude extract, fiber removal by filtration, clarification by filtration, capture by affinity Protein A membrane chromatography, intermediate purification by ceramic hydroxyapatite chromatography, polishing by virus …
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Indole diterpenoid secondary metabolites produced by the Epichloë festucae var. lolii – Lolium perenne symbiosis.
… diterpenoid intermediates of lolitrems in a crude extract of Lolium perenne (L. perenne) seed infected with Epichloë festucae var. lolii (E. festucae var. lolii) and their biological activity against porina larvae were investigated. Liquid-liquid partitioning of the crude extract, together …
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Pharmacological studies of bioactive compounds from medicinal mushrooms in Northeast of Thailand
… everhartii, P. laevigatus and F.cajanderi were extracted with ethanol and biological evaluation such as anti-cancer, anti-Alzheimers, anti-microbial and anti-inflammatory activity carried out. An ethanol crude extract of F. cajanderi showed cytotoxicity against ovarian carcinoma (A2780), …
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Troubleshooting, optimization and robustness testing of CeBER process for c-phycocyanin extraction and purification from spirulina
… leaching, bead-milling did increase the initial extraction rate of c-phycocyanin from dried Spirulina powder. However, it was found that bead-milling may have no effect in the overall leaching time taken to reach an equilibrium concentration of c-phycocyanin in the crude extract. Bead-milling was …
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Analysis of actinobacterial biodiversity in reservoir sediment and cave soil and screening of isolates for antimycobacterial activity
… activity, were selected for antibiotic extraction from culture broths. The resulting crude extracts were subjected to spot bioautography to test for antibacterial activity. The organic compounds extracted from the cell mass of Streptomyces strain CS3 and the broth fraction of …
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Bioassay-guided fractionation of Artemisia afra for in vitro antimalarial activity against Plasmodium falciparum
… this species for antimalarial activity. Crude petroleum ether soxhlet extracts of Anemisia afra had demonstrated antimalarial activity against Plasmodium falciparum, FCR-3, cultured in vitro. The IC₅₀ values ranged from 5-13μg/ml. The extract from leaves and flowers was then screened …
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Culturing and processing of Dunaliella for enrichment of enzymatic antioxidants
… (wet whole cells, freeze-dried whole cells, crude extract, freeze-dried crude extract) were examined. Short-term cultivation (>2 days) of D. tertiolecta at >20°C under low to medium light irradiation and with nitrogen concentration >0.5 mM KNO3 stimulated CAT activity, as did prolonged …
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Isolation, Structure Elucidation, and Synthesis of Natural Products from Marine Cyanobacteria
… of natural products from marine cyanobacteria. A crude extract from a cyanobacterium collected in Curacao showed selective affinity for the dopamine D<sub>5</sub> receptor in a screen against a panel of CNS receptors. Due to the high similarity of the D<sub>5</sub> and D<sub>1</sub> receptor, to …
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Antibiotic Potential of Hericium and Hohenbuehelia Species
… lugdunensis, and Micrococcus luteus, with crude extracts being tested against the latter three. Multiple strains from both genera demonstrated antibiotic activity, with activity against Gram-positive bacteria (S. aureus, S. lugdunensis, and M. luteus). Hericium abietis and Hericium …
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Isolation and Characterization of Active Ingredients from Nigella Sativa for Antibacterial Screening.
… the various antibacterial activities of total extract from the seeds of <em>Nigella sativa</em> (Black cumin). In attempts to identify the active ingredients in this extract, the seeds were extracted with hexane. The volatile oil obtained from the crude extract was shown to contain at least …
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Mechanisms of Immunosuppression in Murine Malaria
An extract was prepared from Plasmodium berghei, NK65C strain, by disrupting P. berghei infected erythrocytes with a Parr bomb disruption cell. This extract was capable of reducing the anti-DNP and SRBC plaque forming cell response of mice intraperitoneally injected with the extract one day prior …
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In vitro evaluation of the enzyme inhibition and membrane permeation properties of benzophenones extracted from honeybush
… (i.e. lipase and α-glucosidase) effect of crude honeybush extracts, benzophenone rich fractions and xanthone rich fractions from Cyclopia genistoides. The in vitro permeation of marker molecules (i.e. benzophenone and xanthone molecules) after application of the crude extracts and fractions …
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Isolation of Natural Products from Casearia nigrescens
… Cooperative Biodiversity Group (ICBG), plant extracts were received from Madagascar. The extracts were screened for cytotoxicity using the A2780 human ovarian cancer cell line bioassay. The crude extract of Casearia nigrescens was fractionated and yielded five known compounds and one new …
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Exploring the molecular diversity and biomedicinal potential of marine invertebrates and South African actinomycetes for tuberculosis drug discovery
… Several organic and aqueous marine invertebrate extracts were screened for their in vitro inhibitory activity against the Mtb strain H37Rv. The chemical components of two out of 54 active extracts were dereplicated using 1H NMR, HR-LCMS with GNPS molecular networking. The extracts were …
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Construction and Use of Species-Specific Dna Probes for the Detection, Identification, and Enumeration of Human Colonic Bacteroides Species (Anaerobes, Hybridization, Microecology, Feces)
… and enumeration of Bacteroides species in a crude extract from feces. The concentrations of these organisms in feces that were estimated by the DNA probe method are in agreement with estimates that have been previously obtained by conventional bacteriological methods. Moreover, with the DNA …
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