Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 9 of 9 for “"Crosslinking Mass Spectrometry"”.
-
Quantitative crosslinking mass spectrometry
… difficult to monitor by existing technologies. Crosslinking mass spectrometry (CLMS) can provide residue-resolution distance restraints, which may in principle be quantified to obtain unique insights into the structural flexibility of proteins. However, quantitative crosslinking mass …
-
Computational methods and machine learning for crosslinking mass spectrometry data analysis
… structure of proteins and protein complexes. Crosslinking mass spectrometry (CLMS) is a comparatively new tool for the analysis of single proteins, multi-protein complexes, and protein-protein interactions. CLMS poses several challenges for mass spectrometry-based proteomics, which include …
-
The Development and Application of Novel Crosslinking Mass Spectrometry Approaches to Study Protein Structure and Interactions.
North Carolina State University Theses Biochemistry.
-
Top-Down Interrogation And Characterization Of Biological Macromolecules
<p>Analytical mass spectrometry has been widely applied in the field of biomolecule identification and characterization. It provides fast, accurate and robust information relevant to the molecular mass, composition and structure of the target analytes.</p> <p>This thesis is focused on the top-down …
-
MOLECULAR RECOGNITION OF THYROGLOBULIN BY SORTILIN
… approach combining cryo-electron microscopy, crosslinking mass spectrometry, biophysical characterisation, and endocytosis assays in human thyroid cells. We demonstrate that sortilin preferentially binds the monomeric form of TG over its native dimeric state, suggesting a conformational …
-
STRUCTURAL INSIGHTS INTO RNA POLYMERASE III PRE-INITIATION COMPLEX AND THE PIONEER ROLE OF OCT1
… versus Pol II. Through integrative modeling and crosslinking mass spectrometry, we structurally localized the SNAPC2 and SNAPC5 subunits near promoter DNA. These subunits had previously been proposed to act as an autoinhibitory module, and our work provides a structural framework supporting this …
-
Structural and Biochemical Investigation of Fanconi Anemia Pathway Activation
… the FA core complex. Instead, using quantitative crosslinking mass spectrometry, I discovered that phosphomimetic mutations locally alter the D2-I dimerization interface to prime the complex for closure. Thus, phosphomimetic mutations shift the conformational equilibrium of D2-I towards a closed …
-
NEW INSIGHTS INTO THE MOLECULAR PATHOGENESIS OF SEVERE FEVER WITH THROMBOCYTOPENIA SYNDROME VIRUS
… We employed a novel workflow utilizing in-situ crosslinking coupled with mass spectrometry to develop a high-containment compatible system capable of capturing host: pathogen interactions in-situ. Employing this workflow on SFTS virus infected HuH7 cells, we were able to capture 15 interactors …
-
How is Ataxia-Telangiectasia Mutated Protein Kinase Activated?
Ataxia-Telangiectasia Mutated (ATM) is a key protein kinase in the cell’s response to double-stranded breaks in DNA. This damage is detected by the Mre11-Rad50-Nbs1 (MRN) complex, which recruits ATM to the DNA. Upon activation, ATM phosphorylates a vast range of substrates, which triggers a …