Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"Cre-LoxP system"”.
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Using the Cre-loxP system to randomize target gene expression states and generate diverse phenotypes
… state of multiple genes in S. cerevisiae using Cre-loxP recombination. By inserting promoters flanked by inverted loxP sites in front of a gene of interest we can randomly alter its expression by turning it OFF or ON, or between 4 distinct expression states. We show at least 6 genes can be …
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Testing the reliability and selectivity of different bone-cell-specific Cre- expressing mouse models for studying bone cell metabolism
The Cre/loxP system is a tool for targeted recombination of DNA. For applying Cre recombinase-mediated genome modifications, there is a requirement for reliable, high-fidelity, and specific transgenic expression of the Cre recombinase. This study focuses on the reliability of different bone cell …
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Genetic analyses of the Rb and p53 genes in murine development and tumorigenesis
… activity and apoptosis in the central nervous system (CNS), peripheral nervous system (PNS), and ocular lens. Loss of p53 can prevent the apoptosis in the CNS and lens; however, the specific signals leading to p53 activation have not been determined. We tested the hypothesis that hypoxia caused …
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Mouse models of lung cancer : understanding the molecular and cellular basis of lung tumorigenesis
… cancer. Furthermore, there are no effective screening methods for lung cancer and standard chemotherapeutics are ineffective in treating advanced lung cancer. The work presented here describes the development and characterization of a murine lung cancer model that uses conditional expression …
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Genetische Untersuchungen zur Rolle von Cytochrom C und Stat3 bei der Regulation des embryonalen Zelltods von Motoneuronen der Maus
… (EAE) hin. Charakterisierung der NFL-Cre Maus Die zelltypspezifische Genablation mit dem Cre/loxP System umgeht einige der größten Probleme der klassischen Methode der Geninaktivierung in Mäusen, indem nur in bestimmten Geweben oder Zelltypen, eventuell sogar nur ab einem bestimmten …
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Integrinaktivierung und Formation des "fokalen Adhäsionskomplexes" im Hypertrophieprozess adulter Mäuseherzen
… wurde eine konditionale „knock-out“-Mauslinie (Cre/loxP-System mit MLC-2v-Promotor) etabliert. Bei diesen Mäusen ist das beta.1-Integrin-Gen selektiv im Myokard ausgeschaltet. Die Ergebnisse aus Phänotypisierung und Charakterisierung dieser Mauslinie im Hypertrophieprozess stehen noch aus.
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The role of adaptor proteins Crk and CrkL in lens development
… development and fiber elongation, we employed Cre/LoxP system to generate a lens specific knockout of Crk/CrkL. This led to extracellular matrix defects, disorganization of the lens fiber cells, and a defect in lens fiber cell elongation. Deletion of Crk and CrkL in the lens also mitigated the …
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Molecular analysis of transcriptional properties of Pbx1 and E2a-Pbx1
… or aplasia of multiple organs. Utilizing the Cre-loxP system we inactivated both alleles of the Pbx1 gene in the ES cells and mice. We induced neural differentiation of the wild-type and Pbx1 knockout ES cells by aggregation and Retinoic Acid (RA) induction. In order to check for gene …
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Suprachiasmatic nuclei development: A characterization of transcription factors and the influence of retinal innervation and VIP signaling
… with an essential behavioral function; creating the rhythm of the mammalian central clock and entraining that internal clock to the external world. The nuclei each consist of approximately 10,000 neurons, each capable of creating near 24 h rhythms, organized into a highly structured …
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The Physiological Function of Endothelin-2 in Mice
… we generated constitutive, tissue-specific and systemically inducible knockout mice. Global ET-2 deficient mice exhibited severe growth retardation and juvenile lethality. Despite normal milk intake, they suffered from an apparent internal starvation characterized by hypoglycemia, ketonemia, and …
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Roles for the E3 Ligase Itch in Immune Response Modulation
… for conditional deletion in mice using the Cre-loxP system. The results presented in this thesis provide the first direct evidence that Itch deficient CD4+ T cells are sufficient to cause spontaneous inflammation. The function of CD4+CD25+ regulatory T cells was found to be intact in vitro. …
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Novel roles of ADF/cofilins in maintenance of homeostasis in normal and malignant epithelial cells
… with conditional depletion of CFL1 using a Cre-LoxP system under the control of the keratin 14 promoter, we were able to study the effects of ADF/CFL1 loss in vivo in the mouse epidermis. Furthermore, by generating ADF-null squamous cell carcinoma (SCC) cell lines and by transiently …
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FoxO1 in the regulation of adipocyte autophagy and biology
… shown that excess adiposity may arise from increased adipogenesis (hyperplasia) and adipocyte size (hypertrophy) . Emerging evidence underscores autophagy as an important mediator of adipogenesis and adiposity. We are interested in the upstream regulator of adipocyte autophagy and how it …
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The Diversity of Cortical Interneurons
… interneurons are key elements of the cortical system. These interneurons stabilize networks, but at the same time they also add non-linear effects to the excitatory system to make the cortical network more dynamic. To achieve this, cortical interneurons form a very heterogeneous group, making …
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Functional analysis of tcf21 and tbx20 in zebrafish
… method of this in the mouse model utilizes the Cre/loxP system: two loxP sites flank a required segment of a gene, and the introduction of the enzyme Cre deletes the DNA between them. Until CRISPRs this was not feasible in zebrafish, which lacked an efficient method of targeted modification in …
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Physiological Role of the α<sub>2</sub>-Isoform of the Na, K-ATPase in the Regulation of Cardiovascular Function
… the Na,K-ATPase expressed in the cardiovascular system but is thought play a critical role in the regulation of systolic blood pressure (SBP). However, the organ system/cell type expressing α<sub>2</sub> that is required for this regulation has not been fully defined. The present dissertation …
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The Role of Rac1 in Synaptic Plasticity, Learning and Memory
… of synaptic transmission in the central nervous system. Rac1, a protein of the Rho subfamily of GTP binding proteins (GTPases), is largely known for its involvement in cytoskeleton remodeling, and has been implicated in neuronal development, participating in the morphological changes required for …
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THE MOLECULAR AND BEHAVIORAL CHARACTERIZATION OF THE MURINE CRE – LOXP SKELETAL MUSCLE SYNTHESIZED BDNF KNOCKOUT MODEL
… within the central and peripheral nervous systems. BDNF is also a member of the myokine family, where muscle synthesized-BDNF (msBDNF) is thought to facilitate the crucial bi-directional communication between the motor neuron and the muscle fibers it innervates. Since 2008, the Ottem …