Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"Cre-Lox Recombination"”.
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THE CHARACTERIZATION OF BEHAVIORAL ABNORMALITIES IN BDNF LOXP TRANSGENIC MICE
<p>BDNF homozygous floxed mice (BDNF<sup> lox+/+</sup>) are a transgenic mouse strain used to study the neurotrophin brain-derived neurotrophic factor (BDNF) through Cre-Lox recombination when crossed with the appropriate Cre-expressing strain. BDNF<sup> lox+/+</sup> mice contain two artificially …
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The effect of brain-specific VEGF-C deletion on vascular morphology in the brain and skull
… role of VEGF-C in the brain, we generated Nestin-Cre; Vegfcfl/fl mice, in which we confirmed deletion of Vegfc. Nestin-Cre is expressed in neuronal and glial cell precursors, in which it mediates Cre-lox recombination of the floxed Vegfc allele. The objective of my study was to see if …
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Abscisic acid ameliorates glucose tolerance and obesity-induced inflammation
… a tissue-specific knockout generated through Cre-lox recombination, we were able to determine that this effect was dependent on PPARgamma in immune cells. To further characterize the differences between the ATM subsets that were affected by ABA, we performed a multi-organ assessment (i.e., …
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Distinct roles for inhibitory neuron subtypes in cortical circuits : an examination of their structure, function, and connectivity
… are the targets of PV+ and SOM+ neurons? We used Cre-lox recombination to introduce either fluorescent protein or channelrhodopsin to PV+ or SOM+ neurons, targeting these cells for two-photon targeted physiological recording and morphological reconstruction, or selectively stimulating the …
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DYRK1A-Related Trabecular Defects in Male Ts65Dn Mice Emerge During a Critical Developmental Window
… To tests this hypothesis, doxycycline-induced cre-lox recombination to reduce Dyrk1a gene copy number or the DYRK1A inhibitor CX-4945 began at P21. The results of both genetic and pharmacological interventions suggest that trisomic Dyrk1a does not influence the emergence of trabecular defects …
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Development and applications of programmable DNA-guided Argonaute-based artificial restriction enzymes
… as AREs. Through these studies, I was able to create an engineered version of PfAgo enzyme which demonstrates lower non-guided nuclease activity as well as higher specificity for cleavage of high GC-content DNA sequences compared to the wild-type PfAgo enzyme. To demonstrate some of the …
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Measurement of Efficiency of Cre-mediated Recombination between lox loxSites Using a New Vector System
PURPOSE: To measure the Cre-mediated recombination efficiency between 'lox sites' using a new vector system. MATERIAL AND METHODS: A new vector (pFGB) to analyze Cre-mediated recombination efficiency between two lox sites was constructed. The combinations of wild type and mutant lox sites that are …
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SITUATION MOUSE ALL FLOXED UP: EXPLORING PHENOTYPIC ABNORMALITIES IN BDNF FLOXED MICE
… Ottem Laboratory became concerned when mice with LoxP sites flanking the BDNF gene depicted anxiety-like behaviors such as aggression, difficulty handling, and increased agitation. Though many studies have noted behavioral and phenotypic abnormalities related to large gene inserts, few have noted …