Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 57 for “"Cre recombinase"”.
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Testing the reliability and selectivity of different bone-cell-specific Cre- expressing mouse models for studying bone cell metabolism
The Cre/loxP system is a tool for targeted recombination of DNA. For applying Cre recombinase-mediated genome modifications, there is a requirement for reliable, high-fidelity, and specific transgenic expression of the Cre recombinase. This study focuses on the reliability of different bone cell …
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Directed evolution of an HIV-1 LTR specific recombinase for anti-retroviral therapy- a proof of concept study
… presented in this thesis has been to engineer Cre recombinase to recognize and recombine a sequence from an HIV-1 Long Terminal Repeat (LTR), characterize the recombination proficiency of the evolved recombinase in mammalian cells and explore the potential of the recombinase for a novel …
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Characterization of Tyrosine Hydroxylase-Positive Neurons Projections from the Paraventricular Nucleus of Hypothalamus to Hindbrain Autonomic Regulatory Centers
… regulating centers. To achieve our goal, Cre recombinase-dependent TIT2L-eGFP reporter mice were microinjected with adeno-associated virus encoding the Cre recombinase driven by rat TH promoter (AAV2-rTHP-Cre, 6.8 x 1011 gc) into the PVN using a stereotaxic apparatus. One month after …
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Towards a more ethical animal model in biomedical research
… ablated before an experiment. An ERT2-inducible Cre recombinase under the Wntl promoter was designed to be combined with a floxed Nav1.7 ion channel mouse model. When a 4- hydrotamoxifan class small molecule is fed to the mouse, Cre recombinase expression in the peripheral nervous system will …
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Mash1 Defines Lineage Restricted Neuronal and Oligodendrocytic Precursor Cells in Spinal Cord Development
… mouse strains were generated that express GFP, Cre recombinase, and tamoxifen-inducible Cre recombinase. The M1-GIC mouse line, showed faithful Mash1 expression recapitulation and traces Mash1+ progenitors mainly to dI3 and dI5 interneurons. This supports data from the Mash1 null mutant where …
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Development and characterization of immunogenic genetically engineered mouse models of pancreatic cancer
… of immunotherapy in many cancers. However, pancreatic cancer has remained largely refractory to checkpoint immunotherapy. To uncover mechanisms of immune escape, we have characterized two preclinical models of immunogenic pancreatic ductal adenocarcinoma (PDAC). In order to dissect the …
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Investigations of lipid metabolism in Yarrowia lipolytica
… gene deletion method using a combination of Cre recombinase and the AHAS* gene was synthesized, developed, and tested successfully. This knockout system allows for serial deletion of genes with the use of only one resistance marker, with excision of the marker after selection.
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Development and application of control tools for use in optogenetics research
… in conjunction with a mouse that expresses the Cre recombinase enzyme in a specific cell type, cell specific expression of the opsin is achieved. When used with LEDs, lasers, or specifically fabricated light delivery tools, control of very specific neural networks is realized. This thesis …
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Postnatal specialization of astrocyte regional heterogeneity in the mammalian brain and improved tools for studying glia
… the generation and characterization of Gpr17-Cre, a novel Cre recombinase driver line sensitive and specific for the oligodendrocyte lineage and a subset of astrocytes in the central nervous system.
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Mouse models of lung cancer : understanding the molecular and cellular basis of lung tumorigenesis
… cancer. Furthermore, there are no effective screening methods for lung cancer and standard chemotherapeutics are ineffective in treating advanced lung cancer. The work presented here describes the development and characterization of a murine lung cancer model that uses conditional expression …
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Exploring the role of Gastrin-Releasing Peptide Neurons in Circadian Time-Keeping of the Suprachiasmatic Nucleus
… (SCN), which provides internal time cues to create coherent circadian rhythms of physiology and behaviour. Time-keeping in the SCN operates at two levels: the intra-cellular, cell-autonomous transcriptional/translational feedback loop (TTFL) and the intercellular network, in which …
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Exploring the role and the function of Aryl Hydrocarbon Receptor (AhR) and Aryl Hydrocarbon Nuclear Translocator (ARNT) in T cells
… AhR signaling. In this model a mouse expressing Cre recombinase ‘knocked in’ to the CYP1A locus (CYP1A1Cre+/-) was backcrossed to the R26REYFP reporter mouse. In R26REYFP mice, a gene encoding EYFP is knocked into the ubiquitously expressed Rosa26 locus preceded by a loxP flanked stop sequence. …
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Understanding Genomes Through Engineered Structural Variation
… editors to insert recognition sequences for recombinases at high throughput to engineer genomes at an unprecedented scale. In the process, I made discoveries about the biology of genome engineering, structural variation, and gene regulation. I first outlined the determinants of short sequence …
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Cell-specific roles for CASK in the pathology of Optic Nerve Hypoplasia
… (CASKfl/fl) with transgenic mice that express Cre Recombinase (Cre) in RGCs. Deletion of CASK from RGCs did not further alter ONH size nor RGC survival. These results demonstrate that loss of CASK signaling in this discrete neuronal populations is not sufficient to lead to further disruption in …
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The Interferon Stimulated Gene Product Lymphocyte Antigen 6 Complex, Locus E Promotes Entry of a Subset of Diverse RNA Viruses and Inhibits Infection by Coronaviruses
… hundreds of IFN-stimulated genes (ISG). In a screen to identify novel antiviral factors, the Schoggins lab unexpectedly uncovered a subset of genes that enhanced viral infection. Here, I describe my personal efforts to study lymphocyte antigen 6, locus E (LY6E), a protein which was identified …
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Naᵥ1.8+ Visceral Afferent Neurons: Roles in Metabolism and Inflammation
The increasing prevalence of obesity and its related comorbidities are major health issues facing western societies. Obesity involves a long-term, chronic perturbation of energy balance, in which food intake is not properly matched to calories expended. It is also closely linked with low-grade …
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The role of Xist RNA in the maintenance of X chromosome inactivation
… with an adenovirus vector carrying the gene for Cre recombinase. After Cre mediated deletion of Xist, the inactive X remained transcriptionally silent, late replicating, and hypoacetylated on histone H4, confirming that X-inactivation can be maintained in the absence of Xist RNA. However, the …
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Removal of Pax6 from cortical progenitors renders them abnormally likely to switch fate in response to Sonic Hedgehog activity
… mice expressing a tamoxifen-inducible form of Cre recombinase under the control of the Emx1 locus (Emx1-CreERT2) and an EGFP reporter construct. Cultured cortical progenitors were exposed to increasing concentrations of Shh agonist, developed for the protein Smoothened (SAG), which is a key …
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Integrins in muscle disease and repair
… in skeletal muscle. In this study we show that increased levels of the adult extracellular variant X2 interfere with muscle integrity, while the presence of embryonic integrin α7 extracellular variant X1 results in normal skeletal muscle architecture. Furthermore, detailed analysis of mdxα7tg mice …
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