Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 55 for “"Cre/loxP"”.
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Using the Cre-loxP system to randomize target gene expression states and generate diverse phenotypes
… state of multiple genes in S. cerevisiae using Cre-loxP recombination. By inserting promoters flanked by inverted loxP sites in front of a gene of interest we can randomly alter its expression by turning it OFF or ON, or between 4 distinct expression states. We show at least 6 genes can be …
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THE MOLECULAR AND BEHAVIORAL CHARACTERIZATION OF THE MURINE CRE – LOXP SKELETAL MUSCLE SYNTHESIZED BDNF KNOCKOUT MODEL
… has investigated the role of msBDNF by employing Cre/LoxP recombinase technology to selectively delete the BDNF coding region within skeletal muscle of mice. This approach generates animals with either heterozygous or homozygous deletions of msBDNF, as well as BDNF homozygous floxed mice …
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Testing the reliability and selectivity of different bone-cell-specific Cre- expressing mouse models for studying bone cell metabolism
The Cre/loxP system is a tool for targeted recombination of DNA. For applying Cre recombinase-mediated genome modifications, there is a requirement for reliable, high-fidelity, and specific transgenic expression of the Cre recombinase. This study focuses on the reliability of different bone cell …
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Genetische Untersuchungen zur Rolle von Cytochrom C und Stat3 bei der Regulation des embryonalen Zelltods von Motoneuronen der Maus
… (EAE) hin. Charakterisierung der NFL-Cre Maus Die zelltypspezifische Genablation mit dem Cre/loxP System umgeht einige der größten Probleme der klassischen Methode der Geninaktivierung in Mäusen, indem nur in bestimmten Geweben oder Zelltypen, eventuell sogar nur ab einem bestimmten …
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MOLECULAR MECHANISMS OF APPETITE AND ENERGY BALANCE
… zone protein, ELKS1 and ELKS2, through Nkx2.1-cre-loxP recombination. Nkx2.1 is a developmental progenitor of hypothalamic nuclei; while the loss of ELKS proteins has been shown to reduce neurotransmitter release at both inhibitory and excitatory synapses. The conditional deletion of ELKS …
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Learning and cemory in the cav1.2 knockout mouse
… learning and memory. Utilizing the Cre/loxP gene-targeting system, the CaV1.2 L-VGCC isoform was conditionally deleted in the forebrain of mice. This extensive deletion was confirmed by RT-PCR and Immunoblotting. To test for spatial learning and memory, a series of Morris water maze …
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Genetic analyses of the Rb and p53 genes in murine development and tumorigenesis
… genes in CNS tissue from Rb-l- embryos. The Cre-loxP system was used to generate embryos in which Rb was deleted in the CNS, PNS and lens, in the presence of normal erythropoiesis.
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Transposable prophage Mu exists as an independent chromosomal domain in E. coli
… by segregation into 400-500 supercoiled domains, created by both active and passive mechanisms like transcription and DNA-binding proteins. We find that transposable prophage Mu, transcriptionally silent by definition, is organized into an independent domain as determined by the close proximity of …
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The Role of Sonic Hedgehog in the Hypothalamus
… J. Epstein Sonic hedgehog (Shh) is a morphogen secreted during early development that is required for the formation of the ventral neural tube, including the ventral forebrain. The prechordal source of Shh underlying the forebrain is required early to bifurcate the cerebral hemispheres and eye …
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Mouse models of lung cancer : understanding the molecular and cellular basis of lung tumorigenesis
… cancer. Furthermore, there are no effective screening methods for lung cancer and standard chemotherapeutics are ineffective in treating advanced lung cancer. The work presented here describes the development and characterization of a murine lung cancer model that uses conditional expression …
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Investigating immune surveillance, tolerance, and therapy in cancer
… multiple elements of an intact immune system. We created a new system that is compatible with Cre-loxP-regulatable mouse cancer models in which the defined antigen SIY is specifically over-expressed in tumors, mimicking clinically-relevant tumor-associated antigens. To demonstrate the utility of …
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Analyse Gewebe-spezifischer Deletionen der cGMP-abhängigen Proteinkinase Typ I in Mäusen
… somatischen Mutagenese mit Hilfe des Cre/loxP-Rekombinationssystems umgangen werden. Diese Technik erm?xF6;glicht es, ein Gen Zeit- und Gewebe-spezifisch auszuschalten und damit den schwerwiegenden Ph?xE4;notyp der konventionellen cGKI Mutanten zu umgehen. Ziel der Arbeit war es, …
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Analysis of Spacer Region derived from Recombination between Heterologous loxPs
… 부위특이적 재조합 (site-specific recombination) 방법의 하나인 Cre-loxP 시스템에서, mutant loxP 간의 재조합 반응 시 spacer region 염기 서열을 분석한다. 또한 heterologous (mutant) loxP 간의 반응 시 spacer region의 염기 서열 분석을 통하여 Cre 매개 재조합 메카니즘의 이해를 넓힌다. 재료 및 방법 : 두 lox site 사이의 Cre 매개 재조합 반응으로 나타나는 spacer region의 염기 서열 분석을 위하여 새로운 벡터 pFGB를 …
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Identifikation des mitochondrialen Proteins Frataxin als stoffwechselmodulierenden Tumorsuppressor
… Frataxin in Mäusen erreicht. Der durch das Cre-/loxP-Rekombinasesystem erreichte organspezifische Knock-out wurde auf Transkriptions- und Translationsebene nachgewiesen. Anhand verminderter Aconitaseaktivität, geringeren Sauerstoffverbrauches und reduzierten ATP-Gehaltes im Lebergewebe wurde …
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Integrinaktivierung und Formation des "fokalen Adhäsionskomplexes" im Hypertrophieprozess adulter Mäuseherzen
… wurde eine konditionale „knock-out“-Mauslinie (Cre/loxP-System mit MLC-2v-Promotor) etabliert. Bei diesen Mäusen ist das beta.1-Integrin-Gen selektiv im Myokard ausgeschaltet. Die Ergebnisse aus Phänotypisierung und Charakterisierung dieser Mauslinie im Hypertrophieprozess stehen noch aus.
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Transgenic use of SMAD7 to suppress TGFß signaling during mouse development
Neural crest cells (NCC) are a multipotent population of cells that form at the dorsal region of neural tube, migrate and contribute to a vast array of embryonic structures, including the majority of the head, the septum of the cardiac outflow tract (OFT), smooth muscle subpopulations, sympathetic …
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The role of adaptor proteins Crk and CrkL in lens development
… development and fiber elongation, we employed Cre/LoxP system to generate a lens specific knockout of Crk/CrkL. This led to extracellular matrix defects, disorganization of the lens fiber cells, and a defect in lens fiber cell elongation. Deletion of Crk and CrkL in the lens also mitigated the …
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Molecular analysis of transcriptional properties of Pbx1 and E2a-Pbx1
… or aplasia of multiple organs. Utilizing the Cre-loxP system we inactivated both alleles of the Pbx1 gene in the ES cells and mice. We induced neural differentiation of the wild-type and Pbx1 knockout ES cells by aggregation and Retinoic Acid (RA) induction. In order to check for gene …
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Transcription factor Pax6 protects mouse cortical cell fate specification
… investigated the postnatal characteristics of a Cre-loxP recombinant mouse that had Pax6 removed from the Emx1-lineage at E11.5. I targeted L5 and L2/3 of the somatosensory cortex and recorded their intrinsic electrophysiological properties. The results suggested that PCs can be specified without …
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Role of SH3 and Cysteine-Rich Domain 3 (STAC3) in Skeletal Muscle Development, Postnatal Growth and Contraction
… depolarization, and caffeine and 4-chloro-m-cresol (4-CMC) activation of ryanodine receptor (RyR). Frequent electrostimulation-, high [K+]-, 4-CMC- and caffeine-induced maximal tensions in Stac3-deleted muscles were approximately 20%, 29%, 58% and 55% of those in control muscles, …
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