Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 37 for “"CpG islands"”.
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DNA methylation analysis and identification of a novel antisense transcript in the Peg3 imprinted domain
… domain. We investigated this region for both CpG islands and long antisense transcripts, two common features of imprinted gene clusters. First, we performed a systematic survey of DNA methylation status of the CpG islands in this region of the mouse, cow, and human genomes. We identified two …
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Regulation of PAX6: DNA methylation and MicroRNAs
… the PAX6 gene, specifically focusing on promoter CpG islands (Study 2a), exons (Study 2b) and intronic CpG islands (Study 2c). In neural induction of human pluripotent stem cells, PAX6 gene expression is first activated, and then repressed. Using targeted bisulfite sequencing and …
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Investigation of candidate imprinted tumour suppressor genes
… their association with differentially methylated CpG islands.
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The Effect of the Microbiome on DNA Methylation
… by hyper-methylation of normally unmethylated CpG islands, and global DNA hypomethylation. Microbiota-free mice are known to live longer than their normal counterparts, and microbial dysbiosis is known to be a hallmark of colorectal cancer. In order to determine the microbiota’s ability to …
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DISCOVERY OF NOVEL EPIGENETIC MECHANISMS OF CARCINOGENESIS BY GENOME-WIDE PROFILING OF NON-CODING REGULATORY ELEMENTS
… is likely a relatively rare event at CpG-islands, and that most CpG-islands are probably silenced by alternative mechanisms, specifically the acquisition of repressive histone marks. With respect to distal regulatory elements, enhancer elements are especially attractive candidates to …
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CHARACTERIZATION OF DNA METHYLATION PATTERNS IN HUMAN EMBRYONIC STEM CELLS AND COLORECTAL CANCER USING WHOLE GENOME BISULFITE SEQUENCING
… knowledge is restricted to gene promoters and CpG islands (CGIs) which were thought to be key regulators of epigenetics. However, less than 7% of CpGs are located within the CGIs, many CpGs remain uninvestigated resulting in a highly fragmented view of the epigenome. Here, I applied whole …
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Impact of the gut microbiota on DNA methylation in colorectal cancer
The CpG Island Methylator Phenotype (CIMP) is a distinct form of aberrant DNA methylation in cancer, and it is seen in 20-40% of colorectal cancers (CRC) where its causes remain elusive. Intestinal microbiota represents an important environmental component implicated in CRC development. …
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Physical mapping of a 2-Mb region centered at D10S94, a locus very tightly linked to the multiple endocrine neoplasia type 2 gene(s)
… gel electrophoresis revealed a dense cluster of CpG islands at D10S94. Genes potentially associated with these CpG islands represent candidate genes for MEN2. To determine whether relatively large sequence alterations, which could serve to direct gene cloning efforts to a small region, were …
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Molecular Characterization of Mammalian De Novo DNA Methyltransferase Chromatin Recruitment
<p>Precise deposition of CpG methylation is critical for mammalian development and tissue homeostasis and is often dysregulated in human diseases. The genomic localization of de novo DNA methyltransferases 3A (DNMT3A) and 3B (DNMT3B) is facilitated in part by chromatin "reader" domains to establish …
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Application of High-Performance Liquid-Chromatography Mass-Spectrometry Platform to Study Metabolism and Epigenetic Control of Metabolism
… DNA methylation mainly occurs in the CpG islands of the promoter region of genes. It is believed that during cancer development de novo DNA methyltransferases methylate tumor-suppressing genes allowing cancer cells to proliferate uninhibited. There are two de novo DNMTs, DNMT3A, and …
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Analysis of methylation status of CGB genes in common nontrophoblastic cancer
… to establish the region of interest and predict CpG islands (CGIs) within the promoter region of the target genes. Methylation-sensitive PCR and next generation sequencing were used to assess CGB3-9 promoter methylation in the cancer cell lines. The transcription level of CGB3-9 genes was …
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Aberrant DNA methylation as a diagnostic and predictive marker of ovarian cancer
Aberrant methylation of CpG islands (CGIs) is associated with transcriptional silencing of key tumour suppressor genes in cancer and is a frequent epigenetic event in epithelial ovarian cancer (EOC). The methylation status of 24 CGIs in a retrospective group of 142 EOCs and 16 non-tumour adjacent …
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Investigating the role of chromatin modifications in CRISPR/Cas9 gene editing
… mutagenesis. Cas9 was targeted to imprinted CpG islands in F1 hybrid mouse embryonic stem cells, and then allele-specific mutation patterns were characterised following high throughput amplicon sequencing. Using this novel system, we discovered that heterochromatin can impede mutagenesis with …
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Accumulation of perfluorooctanoic acid in intestine and lung tissues and its effect on gene expression across multiple pathways
… Ace2 and Tmprss2 genes were upregulated where CpG islands in regulatory region of Tmprss2 exhibit significant hypomethylation patterns. Collectively, these findings suggest that PFOA is potentially contributing to the tissue specific toxicological effects.
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Identification of Factors Involved In Dna Methylation of Cpg-Island-Promoters
… increased DNA methylation levels at promoter CpG islands (CGI). About one-fourth of empirically defined human promoters are surrounded by or contain clustered repetitive elements. It was previously observed that a sharp transition of methylation occurs between highly methylated repetitive …
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Deconvolute Brain Tumor Genomic Alterations Based On Dna Methylation
… associated with genome-wide hypermethylation of CpG islands in gliomas. But other subtype-associated alterations, including telomerase reverse transcriptase (<em>TERT</em>)<em> </em>promoter mutation, alpha thalassemia/mental retardation syndrome X-linked (<em>ATRX</em>) mutation, chromosome …
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Characterization of cancer and aging using multi-omics data
… 조직에서 확인 한 127개의 시그너처는 X 염색체에 많이 분포 되어 있었다. 특히, CpG islands (CGI) 영역에서는 구아닌(G) 과 시토신(C) 염기가 많이 분포된 나이에 따른 과 메틸화 영역이 많았고, CGI를 제외한 non-CGI 영역은 아데닌(A) 과 티민(T) 염기가 많이 분포된 나이에 따른 저 메틸화 영역이 많았다. 반면에, 종양 샘플에서 26개의 시그너처를 추출하였고, 정상 시그너처와의 겹치는 영역은 발견되지 않았다. 정상 시그너처에 존재하는 유전자의 생물학적 과정에 대한 온톨로지를 살펴 보면, 대사 …
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Taz As A Regulator of Mesenchymal Transformation and Clinical Aggressiveness In Gliomas
… to lower grade gliomas. We also identified CpG islands in the TAZ promoter that are methylated in most lower grade gliomas, but not in GBMs. TAZ-methylated glioma stem cell (GSC) lines treated with a demethylation agent showed an increase in mRNA and protein TAZ expression; therefore, …
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Studies on the mammalian piRNA pathway in cancer cells
… defects, including the hypermethylation of CpG islands (CGI). The mechanism underlying aberrant CGI methylation, which often silences genes whose inactivation favours tumour growth, remains unknown. CGI hypermethylation occurs against a background of global hypomethylation, in which …
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Genetic and epigenetic characterization of pediatric high hyperdiploid acute lymphoblastic leukemia
… methylomes. Analysis of promoter-specific CpG islands demonstrated that several B-cell- and neoplasia-associated genes were hypermethylated and underexpressed. Methylation hotspots were associated with chromosome bands predicted to harbor imprinted genes, suggesting that regions rich in …
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