Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 30 for “"CpG island"”.
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Identification of Factors Involved In Dna Methylation of Cpg-Island-Promoters
… increased DNA methylation levels at promoter CpG islands (CGI). About one-fourth of empirically defined human promoters are surrounded by or contain clustered repetitive elements. It was previously observed that a sharp transition of methylation occurs between highly methylated repetitive …
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An investigation of genome-wide promoter region cytosine-phosphate-guanine (CpG) Island methylation profiles in patients with chronic hepatitis B virus infection
… host promoter Cytosine-phosphateGuanine (CpG) islands. This would activate or silence genes including tumour suppressors and result in the clinical disease phenotypes of hepatic inflammation, fibrosis and HCC that characterise chronic HBV infection. Genome-wide microarray analysis was used …
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DNA methylation analysis and identification of a novel antisense transcript in the Peg3 imprinted domain
… domain. We investigated this region for both CpG islands and long antisense transcripts, two common features of imprinted gene clusters. First, we performed a systematic survey of DNA methylation status of the CpG islands in this region of the mouse, cow, and human genomes. We identified two …
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Impact of the gut microbiota on DNA methylation in colorectal cancer
The CpG Island Methylator Phenotype (CIMP) is a distinct form of aberrant DNA methylation in cancer, and it is seen in 20-40% of colorectal cancers (CRC) where its causes remain elusive. Intestinal microbiota represents an important environmental component implicated in CRC development. …
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Epigenetic Regulation of Nuclear Hormone Receptor DAX-1
… sought to determine whether promoter region CpG island methylation and expression of DAX-1 were inversely related , and if the loss of DAX-1 expression in the breast cancer cell line MCF-7 is due to epigenetic mechanisms. Using gene expression assays and bisulfite sequencing, we have …
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A candidate gene based investigation of aberrant DNA methylation in the pathogenesis of primary cutaneous T-cell lymphoma
… to the positional hypermethylation of five CpG din-ucleotides in the Fas CpG island. PLS3, an actin-binding protein not normally expressed in any haematopoietic cell, was aberrantly expressed in malignant cells from 21/35 SS patients and 3/8 MF patients with demonstrated clonal blood …
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Epigenetic silencing of BRCA1 and maspin in sporadic breast cancer
… cytosine methylation of the BRCA1 and maspin CpG island promoters is a common event in the inactivation of these genes in sporadic breast cancer cell lines. Furthermore, we show that the methylation-associated inactivation of BRCA1 occurs in 15-30% of sporadic breast cancer patient specimens …
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Impact of the DNA methyltransferase inhibitor, 5-azacytidine, on chondrocytic phenotype
… with demethylation of the promoter and enhancer CpG island. In this chapter, we also mapped the methylation status of the collagen type II CpG island in cartilage, bone, and several chondrogenic and non-chondrogenic cell lines. The second study (Chapter 4) was prompted by the study of Zimmermann …
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An investigation of the contribution of cigarette smoking and human papillomavirus infection to the epigenetic modulation of cellular genes in cervical epithelium
… associated with HPV infection I performed CpG island and gene expression arrays on two in vitro models, a HPV replication model and a cervical disease progression model. I went on to show that HPV infection is followed by the up-regulation of the DNA methyltransferase DNMT1 which binds to …
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Novel Small-RNA Mediated Gene Regulatory Mechanisms for Long-Term Memory
… serotonin-dependent methylation of a conserved CpG island in the promoter of CREB2, the major inhibitory constraint of memory in Aplysia, leading to the persistence of long-term synaptic facilitation. Taken together, these findings provide a new serotonin-dependent, bidirectional, small-RNA …
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The Analysis of the MCF7 Cancer Model System and the Effects of 5-AZA-2'-Deoxycytidine Treatment on the Chromantin State Using a Novel Microarray-Based Technology for High Resolution Global Chromatin State Measurement
… dC treatment showed a strong correlation with CpG island-based regulation (p < 0.0001), and a restoration of transcription patterns associated with normal mammary tissue. Analysis using splice-form specific microarray probes demonstrated that the chromatin state was not uniform across a gene. …
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Epigenetic effects of benzo(a)pyrene in Fundulus heteroclitus and Danio rerio
… by 55% and gene-specifically reduced promoter CpG methylation in vasa by 17%. As a result, vasa mRNA expression was significantly increased by 33% after exposure. However, BaP at 100 μg/L did not change CpG island methylation or gene expression in Ras-association domain family member 1 …
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The Role of TET Proteins in the Epigenetic Regulation of Neural Gene Expression and Behavior
… in the brains of Tet1Δe4-/- mice. Likewise, the CpG island overlapping the promoter of Oxtr is hypermethylated during early embryonic development and persists into adulthood. We also discovered altered histone modifications at the hypermethylated regions, indicating the loss of TET1 has broad …
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EBV-Associated Gastric Cancer: From Initial Infection to Unique Therapeutic Approaches
… of any tumor type, displaying what is known as a CpG island hypermethylator phenotype (CIMP). EBV infection of B cells can be easily modeled in vitro using primary B cells and lymphoblastoid cell lines (LCLs). However, epithelial cell infection models have proven much more difficult to develop. …
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Aberrations of A Putative Tumor Suppressor Gene Sel1L In Pancreatic Ductal Adenocarcinoma
… somatic mutation, loss of heterozygosity (LOH), CpG island hypermethylation and/or aberrantly expressed microRNAs (miRNAs).</p> <p><strong>Material and methods: </strong>In 42 PDAC tumors, the <em>SEL1L</em> coding region was amplified using reverse transcription polymerase chain reaction …
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Analysis of an epigenetic regulator in mouse embryonic stem cell self-renewal and differentiation
… Trx2 could be involved in the maintenance of CpG island promoter gene expression, thus providing a potent regulatory mechanism for ubiquitously expressed genes.
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Integrative genomic analyses of bacterially-associated colorectal cancer
… cancer, characterised by an increase in CpG island methylation, displays an increased frequency of colonisation by Enterococcus faecalis and by high levels of Fusobacterium. At the pathway-level, this subtype is enriched for pathways related to damage response, infection, inflammation and …
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Investigating The Impact of Intragenic Dna Methylation On Gene Expression, and The Clinical Implications On Tumor Cells and Associated Stroma
… in suppressed autophagy, and demethylation of a CpG island within the gene body of <em>CARD11</em> decreases gene expression. In addition to methylation of the open reading frame portion of a gene, other regions of site-specific DNA methylation along the gene body remain to be explored. Upon …
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Rna-Sequencing Applications: Gene Expression Quantification and Methylator Phenotype Identification
… the application of RNA-seq data to identify the CpG island methylator phenotype (CIMP) by integrating datasets of mRNA expression level and DNA methylation status.</p> <p>Section 1: The cost of DNA sequencing has reduced dramatically in the past decade. Consequently, genomic research increasingly …
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Studies on the Molecular Mechanisms of Tumorigenesis in Neurofibromatosis Type 1
… cells, we had checked methylation status in CpG island on the promotor region of RASA1. But, we could not detect methylation of RASA1. To clarify the cellular functions of p120RasGAP in the malignant NF1 cells, we have used vector-mediated RASA1 gene expression. Western blot analysis after …
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