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Showing 1 to 20 of 34 for “"Coulter counter"”.
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Resolving bacteria with the coulter counter
… of counting and sizing of bacteria with the Coulter Counter and to evaluate orifices which were prepared in this laboratory. Size distributions of Pasteurella multocida and Proteus vulgaris were obtained with the electronic particle counter. These distributions appeared to follow the normal …
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MEMS Coulter counter for dynamic impedance measurement of time sensitive cells
… AT AUTHOR'S REQUEST.] In this research, a MEMS Coulter counter is designed, modeled, fabricated and characterized, which can detect and monitor the dynamic cell impedance changes in situ as a function of time and cellular volumetric changes after mixing isolated cell populations with different …
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The development of a nanoscale Coulter counter for rapid genetic sequence recognition
… of this thesis is the development of a nanoscale Coulter counter for the direct electrical detection of specific genetic sequences of deoxyribonucleic acid (DNA); the general approach used to accomplish sequence recognition is a refinement of the resistive pulse technique. Commercial Coulter …
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Are distinct particle spectra an indication of the state of the phytoplankton community in St Helena Bay?
… coast of South Africa had been analysed with the Coulter Counter and formed the basis of this study. Chlorophyll a content of phytoplankton samples was also measured on monthly cruises and with total particle concentration (determined by the Coulter Counter) showed that overall phytoplankton cells …
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Phytoplankton growth and zooplankton grazing in the southern Benguela current
… of an electronic counting and sizing technique (Coulter counter). A feasibility study was initiated to test the accuracy of the Coulter counter (Model TAII) in estimating phytoplankton size and biomass in fresh and preserved samples from the Benguela current. Counting phytoplankton using this …
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Growth in mixed cultures of microorganisms
… of this interaction was described. A Coulter Counter was used for detecting the number of the microorganisms grown in mixed cultures. Size distribution curves were prepared and used as a means for determining the Coulter Counter settings for counting each organism. Coulter Counter …
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Basic Study Of Micromachined Dep (dielectrophoretic) Manipulator
… the DEP manipulator, preliminary study on a Coulter counter was conducted. The Coulter counter works on the principle of resistive pulse sensing. This counter is used for counting the beads as they pass through the microfluidic channel. Its possible integration with the manipulator was also …
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High precision mass-based assay to examine growth regulation of the cell cycle
… (SMR) to measure mass, and resistive pulse based Coulter counter to measure volume. First, we discuss the implementation of several engineering principles that have enabled the SMR to measure size with a high precision and temporal resolution. As a result, growth rates can now be estimated at a …
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Lipid Micro Emboli in Cardiac Surgery
… of the CPB circuit. This was measured by using a Coulter counter to analyze and count emboli in a series of blood samples. The Coulter counter was shown in the first study to be a valuable technique to evaluate embolic loads of lipid micro emboli in blood. The lungs act as physiological filters …
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Population oscillations in continuous culture of the prey-predator systems Escherichia coli - Dictyostelium discoideum and Proteus vulgaris - Dictyostelium discoideum
… of prey-predator interactions was made. A Coulter Counter was used to enumerate the population levels and periodic checks were performed with a Petroff-Hausser bacterial counts. Population oscillations of both prey and predator occurred in the continuous flow culture of either bacterial …
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Crystalluria in various groups of sportsmen
… formation. Particle size distribution analysis (Coulter counter), ultra-structural analyses (SEM) and urine analysis were conducted. The background to various factors relating to stone formation is discussed as well as the general theory behind the techniques employed. The methods utilized and …
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Wnt Inhibitory Factor (WIF)-1 Promotes Melanogenesis in Normal Human Melanocytes
… The cell proliferation was measured using the coulter counter. The effects on pigmentation were investigated with measurement of melanin contents, tyrosinase activity and its expression. Result: WIF-1 was expressed in melanocytes. WIF-1 inhibited proliferation of human melanocytes in a …
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Physicochemical studies of a novel adjuvant and conjugate vaccines
… called Pheroid™ by use of NMR spectroscopy and Coulter Counter. NMR analysis of Pheroid™ formulations provided a structural fingerprint for the formulations and indicated the relative proportions of the major components present, whereas their particle size distribution was profiled using a …
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The design, development and testing of a non-invasive continuous crystalliser system for the study of calcium oxalate crystallisation processes
… the crystalliser and analysing the product in a Coulter Counter. In this technique, the steady state crystal size distribution is measured by the change in resistance as particles, in an electrically conductive liquid, pass through the Coulter aperture. Due to the large volumes required by the …
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BIOCHEMICAL AND MORPHOLOGIC EFFECTS OF CAVITATION ON NORMAL HUMAN PLATELETS
… conducted. Particle size data obtained with the Coulter counter indicate that cavitation causes both lysis and aggregation. Biochemical assays indicate that release of granular contents occurs, and that platelet functional capacity is irreversibly damaged after exposure to cavitation. Results of …
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The Effect of Infection with Polyoma Virus on the Protein, RNA, and DNA of Mouse Embryo Cell Cultures
… Samples of the cells were counted in a Coulter Counter and the remainder of the cells frozen after centrifugation, in a button. Later, the buttons were thawed and protein, RNA, and DNA analyses were performed on each sample. The micrograms per million cells of protein. RNA, and DNA were …
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A study of the effect of dilution rate on bacterial populations
… patterns in three identical growth vessels. Coulter counts were made on a pure culture of B. cereus grown in a continuous flow system at various dilution rates. These counts did not follow continuous flow theory for a pure culture in that there was no sharp drop in count at the critical …
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Nano-pipette as nanoparticle analyzer and capillary gated ion transistor
… 50 nm to 250 nm. This technique is based on the Coulter counter technique, but has nanometer size pores. It was found that ionic current drops when nanoparticles enter the nanopore of a pulled micropipette, producing a clear translocation signal. Pulled borosilicate micropipettes with opening 50 …
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Kidney stone rarity in South Africa's black population : investigation of the biochemical and physico-chemical properties of Tamm Horsfall Mucoprotein as a possible contributory factor
… aqueous sodium oxalate and was monitored Using a Coulter Counter. In another series of experiments 14C-oxalate was used to initiate crystallization. Monitoring in these experiments was achieved by scintillation counter techniques. Calcium oxalate crystal aggregation was investigated in the …
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Scale-up in emulsion polymerisation
… measured either through photomicroscopy or via a Coulter Counter. The power input to the impeller was also measured. A scale-up procedure was proposed based on the governing relationship between droplet diameter, impeller speed and impeller diameter. The relationships between impeller speed soap …
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