Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"Correlative light and electron microscopy"”.
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Structural and Spatiotemporal Organisation of Bax and Apaf1 during Apoptosis
… insights into the structural, spatiotemporal and functional characteristics of two essential steps of apoptosis, Bax cluster formation and Apaf1 activation, using a combination of biochemical and microscopy approaches. I sought to better understand the contribution of spatial and structural …
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Linking Architecture and Function of Endoplasmic Reticulum - Plasma Membrane Contact Sites
… network of membrane contact sites (MCS) and thereby promote cellular functions such as lipid metabolism, signalling and organelle biogenesis. The formation of cortical endoplasmic reticulum (cER) - plasma membrane (PM) contacts in budding yeast cells is mediated by a set of six conserved …
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Molecular Architecture of the ER-Mitochondria Encounter Structure
… form between all organelles of eukaryotic cells and facilitate interdependent processes between unique subcellular compartments. In budding yeast, MCS between the endoplasmic reticulum (ER) and mitochondria are mediated by a protein complex known as the ER-mitochondria encounter structure …
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Visualisation of dynein complexes in vitro and inside cells
… relies on efficient transport within axons and dendrites, the long processes which extend from neuronal cell bodies. Within neuronal processes, the microtubule cytoskeleton provides tracks for motors to carry cargo over long distances. In axons, cytoplasmic dynein-1 (dynein) is the …
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Testing roles of Hereditary Spastic Paraplegia genes in axonal endoplasmic reticulum modelling in Drosophila
… affect proteins of the spastin, atlastin, REEP and reticulon (RTN) families, which possess hydrophobic hairpin domains in the ER membrane to help confer curvature on tubular ER. Drosophila mutants lacking members of RTN and REEP families exhibit partial ER fragmentation in axons. Conversely, …
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Investigating Microtubules Inside Cells with Cryo-Electron Tomography
… They are important for cell division, shape and motor-driven transport. Axons and dendrites of neurons are filled with longitudinal arrays of microtubules. They serve as tracks for long-range cargo transport driven by the microtubule-based motors kinesin and dynein. This transport can be …
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Investigating the cytoskeleton and microtubule-based transport with in situ cryo-electron tomography
… cytoskeleton is composed of microtubules, actin, and intermediate filaments, which function both as a cellular scaffold and as tracks for motor proteins that drive intracellular transport. Cytoplasmic dynein, in combination with dynactin and activating adaptors, is a microtubule-based motor that …