Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"Core promoters"”.
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Function of core promoters in differential gene regulation during embryogenesis
The core promoter is the ultimate target of all transcriptional regulatory processes. The recently discovered diversity of core promoters and basal transcription factors suggests a regulatory role in differential gene expression. However, the direct contribution of the core promoter remains poorly …
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Developing and applying transient expression systems to identify and understand gene regulatory elements in plants
… the activity of regulatory elements such as core promoters and enhancers. My contribution was essential in assuaging reviewer concerns about prior studies using a different, animal-specific assay design. Using plant STARR-seq, I and my co-author Tobias Jores identify and characterize 75,000 …
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GENOME-WIDE PROTEIN MOLECULAR ARCHITECTURE OF HUMAN PROMOTERS
… (TBP, TFIIB, Pol II), and nucleosomes at promoters. We propose a refined model of human promoters characterized by bidirectional transcription, where two distinct PICs initiate on opposing strands while sharing common upstream regulatory regions. Our findings revealed that different …
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Preinitiation Complex Assembly and General Transcription Factor IIA
… GaI4-DNA binding sites located upstream of two core promoters. The mechanism of activation is investigated by the development of a template competition assay. Using this assay, a partial preinitiation complex containing TFIIB is implicated as a target of GaI4-IE. These experiments, as well as …
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Developing synthetic, minimal promoters in Saccharomyces cerevisiae
Promoters enable synthetic biologists to manipulate protein expression at the DNA level. For this reason, promoters are essential for almost all applications aiming to engineer an organism. Unfortunately, promoters available for eukaryotic organisms are derived directly from the genome. Such …
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The interplay of global chromosomal organisation, promoter-enhancer interactions and transcription
… expression is transcription, which starts at the core promoter region. While core promoters are crucial for transcriptional initiation, they are insufficient for establishing complex tissue- and condition-specific gene expression patterns in multicellular organisms. Additional transcriptional …
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Construction and analysis of novel controllable expression vectors for Bacillus subtilis
… useful promoter-probe plasmid to analyze strong promoters in B. subtilis was established, which allowed to study promoter and mRNA stabilizing elements to enhance the transcript level and mRNA stability leading to higher production of recombinant protein. During the development of the …