Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"Content screening"”.
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OrganixInsights: High throughput imaging and high content screening of organoids
… a comprehensive computational framework for high-content screening and quantitative analysis of 3D organoid and biomedical imaging datasets. The proposed framework integrates deep learning models for image restoration and segmentation with a scalable web-based platform for managing and analyzing …
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Multivariate methods for the statistical analysis of hyperdimensional high-content screening data
… complex datasets. In recent years, high-content screening (HCS) using RNA interference (RNAi) or other perturbation techniques in combination with automated microscopy has emerged as a promising investigative tool to explore intricate biological processes. Image-based HC screens produce …
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Budding yeast cell cycle analysis and morphological characterization by automated image analysis
… cell morphology and yeast cell cycle using high content screening with a high-capacity automated imaging system. The images obtained using this method can also provide information about fluorescently labelled proteins, unlike flow cytometry, which can only measure overall fluorescent intensity. …
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An apparatus for high throughput muscle cell experimentation
… experimentation and could be applied as a high content screening instrument in the pharmaceutical industry providing information at the level of a critical cellular phenotype, myocyte mechanical properties, for drug development and toxicology studies.
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Automatic Computational Techniques for Image Processing Problems
… the method in drug development industry for high content screening. Starting from the traditional statistics-based method, we then investigate a more mathematical model, the total-variation (TV) method for image denoising and deblurring problems. In traditional TV-based models, it is not easy to …
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Novel Neuroblastoma Differentiating Agents
… while reducing the cancer cell viability. A high-content screening (HCS) was conducted to identify hit compounds. This screening measured neurite outgrowth, a phenotypic change which has been described as a marker of differentiation in neuroblastoma cell lines. From this screening, three hits that …
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Bayesian level sets and texture models for image segmentation and classification with application to non-invasive stem cell monitoring
… high throughput analysis, monitoring, and screening of pluripotent and differentiated stem cells, whether human embryonic (hESC), induced pluripotent (iPSC), or animal. Motivated by this and other applications, the Bayesian Level Set (BLS) algorithm is developed for automated segmentation …
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Multi-channel solutions for optical labelfree detection schemes based on the interferometric and grating coupler principle
… of application, namely the high throughput screening and the so called high content screening with two different optical labelfree sensor approaches. Cheap disposable sensor units are of importance for HTS. Here embossed gratings which may act as transducers or coupling elements in the …
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Methods and technologies for high-throughput and high-content small animal screening
High-throughput and high-content screening (HTS and HCS) of whole animals requires their immobilization for high-resolution imaging and manipulation. Here we present methods to enable HTS and HCS of the nematode Caenorhabditis elegans (C. elegans). First we present microfluidic technologies to …
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Development and Application of a Novel Assay for Rapid Identification of Cellular Prion Protein Modulators
… application of an innovative BiFC-based in vitro screening assay, Light-Identification of Protein Suppressors (LIPS), designed for real-time monitoring of PrPC expression and modulation. The LIPS system is based on the Bimolecular Fluorescence Complementation (BiFC) technique, usually used for …
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An integrated approach to ciprofloxacin susceptibility analysis and high-throughput bacterial phenotyping in Salmonella
… concentration (MIC). I have developed high-content imaging methodologies to screen Salmonella grown in the presence of ciprofloxacin. These morphological data suggest that there may be heterogeneous subpopulations with differential responses to ciprofloxacin, which was supported by studying …
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Cross-Modality Profiling of High-Content Microscopy Images with Deep Learning
… metadata labels freely obtained in high-content screening into deep learning architectures. The use of automated microscopy in high-content phenotypic screening of cells treated with compounds or genetic perturbations produces a large amount of high-dimensional data. One example which we …
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A NOVEL PIPELINE FOR DRUG DISCOVERY IN NEUROPSYCHIATRIC DISORDERS USING HIGH-CONTENT SINGLE-CELL SCREENING OF SIGNALLING NETWORK RESPONSES EX VIVO
… work entails the development of a novel high content platform for the measurement of kinetic ligand responses across cell signalling networks at the single-cell level in distinct PBMC subtypes ex vivo. Using automated sample preparation, fluorescent cellular barcoding and flow cytometry the …
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Antibacterial activity of the crude extract and fractions of spirostachys africana against multi-drug resistant bacteria
… biological properties. Methods: Preliminary screening of phytochemical constituents of S. africana and fractions was done using standard qualitative and quantitative methods. Antibacterial activity of the extracts was evaluated using the agar well diffusion method and the microdilution assay …
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TRPML1: Role In Autophagy And Potential Target To Treat Lysosomal Storage Disorders
… complexes. In parallel studies, we used high content (HC) screening approaches to identify small molecules able to ameliorate the MLIV phenotype. In one of them, we tested whether previously identified drugs (150 FDA compounds) inducing TFEB translocation and cellular clearance might be active …
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Functional miRNA-based Phenotypic Screening as a tool to delineate HIV-host interactions and facilitate Novel Drug Discovery
… has remained unknown. A microscopy-based high content screening (HCS) approach was thus developed to systematically evaluate the significance of augmenting or inhibiting the function of individual host miRNAs on the replication dynamics of HIV. A bespoke image analysis and data mining pipeline …
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Pancreatic Cancer - Early Detection, Prognostic Factors, and Treatment
… and Panc-1 (MUC4-) cells, followed by high content screening of protein expression. Results/Conclusion: 134 differentially expressed serum proteins were identified, of which 40 proteins showed a significant up-regulation in the pancreatic cancer group. Pancreatic disease link associations …
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Behind the screen: the hidden digital labor of commercial content moderation
Commercial content moderation (CCM) is the practice of screening of user-generated content (UGC) posted to Internet sites, social media platforms and other online outlets that encourage and rely upon such material to generate visits to and participation from users. Despite being essential to the …