Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 19 of 19 for “"Contact Inhibition"”.
-
Rules of Contact Inhibition of Locomotion for Cell-pairs Migrating on Aligned and Suspended Nanofibers
Contact inhibition of locomotion (CIL), a migratory mechanism, first introduced by Abercrombie and Heaysman in 1953 is now a fundamental driving force in developmental, repair and disease biology. Much of what we know of CIL stems from studies done on 2D substrates which are unable to provide the …
-
CHARACTERIZATION OF HCMV-ENCODED CHEMOKINE RECEPTOR US28 TRANSDUCED MSU1.1 HUMAN FIBROBLAST CELLS
… anchorage independent growth and loss of contact inhibition. Similarly, in the human glioblastoma cell line U373, US28 activated VEGF expression. To determine if US28 is oncomodulatory in normal human cells, we engineered the human fibroblast cell line MSU1.1 to express US28 via lentivirus …
-
Analysis of the function of the Nf2 tumor suppressor protein, Merlin
… disruptions of the actin cytoskeleton, lack of contact inhibition of growth, and anchorage-independent growth. In addition, Nf2-deficient mouse embryo fibroblasts (MEFs) exhibited similar contact inhibition and cell-matrix adhesion defects to Nf2BBA expressing cells. Nf2BBA cells continue to …
-
Development of Bioluminescent Biosensors Monitoring Activity of the Merlin Tumour Suppressor
… share the ability to circumvent or overcome contact inhibition of proliferation (CIP), the process by which cell-cell contacts engage signalling to stop proliferation. Merlin, an ERM-like protein, plays a crucial role in CIP and is inactivated in a wide variety of cancers, suggesting that it …
-
Synthesis and evaluation of novel kinase inhibitors as anti-cancer agents
… their ability to restore cell-cell contact growth inhibition in a RhoA-activated cellline; the general cytotoxicity was measured by their ability to inhibit the growth of K562 andA2780 cells; and the effect on the cell cycle analysed using flow cytometry.Three compounds were …
-
Directed collisions between epithelial cells and fibroblasts in vitro on micromachined substrata
Dental implants contact several cell populations in vivo including fibroblasts (F) and epithelial (E) cells, but there is little information on whether different implant materials or surface topographies alter cell interactions. In this study F and E cells were cultured separately or in combination …
-
Travelling Wave Analysis of a Nonlinear Diffusion Equation
… In many cell lines, cell proliferation is contact-inhibited such that the cell population reaches confluence at long times. Given suitable initial conditions, moving fronts of proliferation and migration can be observed prior to confluence, with two key measurable quantities being the width …
-
Negative Regulation of the Hippo Pathway by the Ajuba LIM Proteins
… Several mechanisms, such as cell-cell contact in vitro and organ size in vivo, have been implicated in the activation of the Hippo pathway, leading to inactivation of the YAP/TAZ transcriptional coactivators and growth arrest. Previous work from our lab has identified the Ajuba LIM …
-
Understanding and Targeting Clearance of Persistent HPV Infections
… exhibited increased proliferation, delayed contact inhibition and commitment to differentiation compared with normal keratinocytes. When the two populations are cultured together, HPV16E6 expressing cells have growth advantage over the adjacent uninfected neighbours in colonising the basal …
-
In Vitro Long Term Time-Lapse Imaging Studies Of The Mcf-10 Mammary Cancer Cell Progression Model Using A Custom Incubator Microscope
… describe a method for quantifying the absence of contact inhibition of growth in cancer cells in cocultures, and I find that cancer cells affect the morphology of normal cells. I find that the cancer cells are better at creating invasive strands in Matrigel, and these invasions are temporally …
-
Cell-cell and cell-medium interactions in the growth of mouse embryonic stem cells
… optimization of, growth in cell types that lack contact inhibition such as cancer cells and stem cells.
-
Physical minimal models of amoeboid cell motility
… biochemistry that controls it. First, we examine contact inhibition of locomotion (CIL), which is one of the ways that cells interact. In experiments, cell migration can be restricted to quasi-one-dimensional stripes. In these stripes, head-on collisions of two cells occur frequently with only a …
-
Beyond Suspension: Light and Growth Dynamics in Immobilised Algal Cultures
… colonies, providing insights into the effects of contact inhibition and micro-gradients of mass transfer. The radial propagation of ring-like oscillations, characterised by variations in parent cell size and chlorophyll autofluorescence emanating from the colony centre, indicated a complex …
-
Regulation of Cell Adhesion By The Ferm Proteins, Ptpn14 and Merlin
… increased E-cadherin membrane movement at cell contacts, disrupting cell contacts and abolishing E-cadherin localization during primordial lumen formation. In cells with established AJs and TJs, decreased levels of PTPN14 led to an increase in β-catenin Y654 phosphorylation, promoting increased …
-
Computer simulations of cellular movements
… of single cells within solid tissue masses; (8) contact inhibition. The results of these simulations are consistent with experimental observations.</p> <p>In addition, a software/hardware package has been developed which allows the visual display of tissue movement on a television screen, with …
-
Repression of E2F-4 Mediated Transcription and Growth by Hypophosphorylated pRb2/p130 in Human Prostate Cancer Cells
… pRb phosphorylation is required to prevent its inhibition of E2F transcription, which would otherwise halt cell cycle progression. E2F transcriptional activity permits the cell to traverse the G1/S restriction point by, among other effects, promoting cdk2 activity, which maintains pocket protein …
-
hCLCA2 IS A p53-REGULATED GENE REQUIRED FOR MESENCHYMAL TO EPITHELIAL TRANSITION IN BREAST
… culture, conditions that cause G1 arrest such as contact inhibition and depriving cells of growth factors and anchorage induced these genes. Therefore, one of the goals of this project was to find if this is true of hCLCA2 in human breast epithelial cells. We found that hCLCA2 was induced by the …
-
NEUROFIBROMATOSIS TYPE 2 PROTEIN (NF2) AS A REGULATOR OF TUMOR SUPPRESSORS AND VIRAL ONCOPROTEINS IN HUMAN GLIOBLASTOMA
… to their cytoplasmic kinases to facilitate contact inhibition. However, NF2 can also interact with an array of cytoplasmic and a few nuclear proteins. To date, little is known about the function of NF2 in tumors not associated with NF2 syndrome. Loss of functional NF2 protein has become a …