Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 33 for “"Conserved Sequence"”.
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Upstream conserved sequence element that regulates TIM-3 induction in CD4+ T cell
… that regulatory DNA regions show nucleotide sequence homology between species, four conserved non-coding sequences (CNS -29, CNS -53, CNS -56 and CNS -63) were analyzed by comparison human TIM-3 upstream DNA sequences with mouse and cow counterparts. Only at CNS -63, H3K4 dimethylation that …
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Investigation of the Molecular Interaction Between Pyr mRNA and the Bacillus Subtilis Attenuation Regulatory Protein, PyrR
… binding. All PyrR-binding mRNAs share a conserved secondary structure, consisting of a lower stem, purine-rich internal bulge, upper stem, and terminal hexaloop, as well as two conserved sequence motifs. Variants that significantly altered the secondary structure of the mRNA disrupted …
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Flavonoid Glucosyltranferases: Cloning and Sequencing of Putative Glucosyltranferases from <em>Citrus paradisi</em> (Grapefruit) Leaves.
… gene specific primer based on a highly conserved sequence area in the Plant Secondary Product Glucosyltransferase box. The objective of this research was to use clone specific primers to obtain 3’ clones of the 3 previously mentioned 5’ clones as well as verify putative GT candidacy …
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Patterns in the sequence context of protein disulfide bonds
… polypeptides was created. The amino acid sequences flanking these disulfide bonds were searched for conserved patterns that may reflect recognition sites by the disulfide bond forming enzyme protein disulfide isomerase (PDI). Several methods of classifying disulfide bonds were explored, …
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Sequencing and functional characterization of a Latrodectus geometricus defensin, Lg-defensin
… analysis and phylogenetic alignment to determine conserved sequence patterns and structural similarities to other defensins. Next, the functionality of Lg-defensin was determined using <em>in vitro </em>RNA interference to assess what impact silencing had on the spider’s ability to control a …
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FROM SERUM TO TISSUE: GALECTIN-BINDING GLYCOFORMS OF SERUM GLYCOPROTEINS AS FUNCTIONAL BIOMARKERS
Galectins are small soluble proteins defined by a conserved sequence motif that forms a defining β-galactoside binding site shared among galectins; adjacent binding sites give each galectin a unique fine specificity for a specific subset of galactose containing glycans. A wealth of data now …
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Improving Profile Similarity Search and Alignment of Protein Sequences
… homologs. Homologous proteins tend to possess conserved sequence motifs, the same structure folds, and similar functional sites. Current sequence-based homology search methods are still unable to detect many similarities evident from protein spatial structures. We present a new method, …
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From Flies to Mice: Drosophila as a Model System to Study Fat Biology
… anti-adipogenic effect of Hedgehog signaling was conserved in mammalian adipogenic models in both sufficiency and necessity tests. Hedgehog signals elicit this function early in adipogenesis, upstream of PPARgamma, potentially acting as a molecular switch diverting preadipocytes and mesenchymal …
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Conservation and synteny of long non-coding RNAs invertebrate genomes and their identification in novel transcriptomes
… diverse range of organisms. Further, I analysed sequence and positional conservation of lncRNAs, demonstrating the presence of short segments of conserved sequence in IncRNAs and the existence of several syntenically conserved non-coding transcripts over large evolutionary distances. However, I …
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A technique to randomise consecutive codons in a sequence of DNA using MAX oligonucleotides
… each of the 20 amino acids that are ligated to a conserved sequence of DNA using T4 DNA ligase. The “MAX” oligonucleotides were synthesised in such a way, with an MlyI restriction site, that restriction of the oligonucleotides occurred after the three nucleotides coding for the amino acids. This …
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Spatial-Temporal Regulation of the Atonal Homolog 1 Gene
… This was accomplished by deleting different conserved regions in a bacterial artificial chromosome (318GFPBAC) containing ~180 kb of sequence 5' and 3' of atonal homolog 1 and assaying for green fluorescent protein expression in transgenic mice. Transgenic embryos were analyzed at embryonic …
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DECIPHERING THE MOLECULAR MECHANISM BEHIND THE SARS-COV-2 FUSION DOMAIN
… of the six-helix bundle, within which a short, conserved sequence known as the fusion domain (FD) initiates the process as it embeds within and perturbs the target cell membrane, in turn lowering the energetic barrier necessary to coalesce two opposing membranes. Furthermore, the highly …
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Peering into Candida albicans pir protein function
… of a repeated motif that encodes the amino acid sequence involved in linking Pir proteins to the cell wall. Despite dire predictions from the literature, the Δpir1/Δpir1 Δpir32/Δpir32 strain was viable and only mildly affected when grown in the presence of compounds that interfere with cell wall …
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Insights into the substrate specificities, interactions and regulatory mechanisms of bacterial glycoconjugate biosynthesis enzymes
… superfamily with a diverse and richly annotated sequence space; however, limited biochemical characterization of this enzyme superfamily has been performed. To close the gap between sequence annotation and biochemical characterization of these critical enzymes, this thesis presents methods for …
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Structure, Mechanism and Chemical Modulation of the Protein Kinase-nuclease Dual-enzyme IRE1
… experiments revealed that the ordered sequence of events: autophosphorylation, nucleotide binding and dimerization; orchestrates the assembly of the IRE1 nuclease active site to potentiate nuclease function. The flavanol quercetin was identified in a chemical screen as a potent …
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Structural RNA Homology Search and Alignment Using Covariance Models
… transport, splicing, and more. Because important sequence and structural features tend to be evolutionarily conserved, one way to learn about functional RNAs is through comparative sequence analysis - by collecting and aligning examples of homologous RNAs and comparing them. Covariance models: …
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Structure and dynamics of Pseudomonas aeruginosa ICP
… fold comprised of seven β-strands. Three highly conserved sequence motifs located in mobile loop regions form the CP binding site. The inhibitor exhibits higher affinity toward the mammalian CP cathepsin L than cathepsins H and B. Homology modelling of the PA-ICP-cathspin L interaction based on …
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Polysomes but not translation are required for estradiol-mediated stabilization of Xenopus laevis vitellogenin mRNA
… may be required to be in close proximity to a sequence and/or secondary structure at the 3$\sp\prime$ end of the vitellogenin mRNA, the four vitellogenin 3$\sp\prime$-untranslated sequences were isolated and sequenced. Sequence comparison reveals a region conserved in both content and position …
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Engineered mRNA regulation using an inducible protein-RNA aptamer interaction
… analyses support a functional role for conserved sequence and structural motifs in TetR binding. We illustrate the utility of this chemically-inducible RNA-protein interaction to directly regulate translation in both a prokaryotic and eukaryotic organism. By genetically encoding …
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Evolution and engineering of protein-protein interactions
… during evolution have important phenotypic consequences that are subject to selection. Therefore, in a crowded cellular environment, proteins must evolve mechanisms to maintain the correct interactions and avoid inappropriate ones. In this work, I leveraged high-throughput methods for the …
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