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Showing 1 to 3 of 3 for “"Conservative substitution"”.

  1. In cellulo and biophysical exploration of the role of GGMP residues of Hsp70

    … and KPfΔG mutants were previously created by conservative substitution and deleting the GGMP residues of KPf, respectively. Circular dichroism and tryptophan fluorescence highlighted that the insertion of GGMP residues did not drastically change the secondary and tertiary structure of DnaK. …

    venda Repository record for In cellulo and biophysical exploration of the role of GGMP residues of Hsp70 (opens in a new tab)

  2. Ester bond formation in bacterial adhesins

    … formation, preventing ester bond hydrolysis. A conservative substitution of the bond forming threonine to a serine produces a hydrolysable variant at high pH, effectively completing the second half of the canonical serine protease mechanism. Wild-type protein at high pH and even in the presence …

    auckland-ms Repository record for Ester bond formation in bacterial adhesins (opens in a new tab)