Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 39 for “"Connexin-43"”.
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Dysregulation of connexin-43 (cx43) in doxorubicin-induced cardiotoxicity
… and dysfunction of gap junctions called connexins in the heart. Here, we examine the effects of the anthracycline DOX on the expression and localisation of connexin-43 (Cx43) in rodent and human cardiac cells and cells of the vasculature to identify if drug treatment impacts this …
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Connexin-32 and Connexin-43 Immunoreactivity in Rodent Taste Buds
… cells via specific hemichannels such as pannexin/connexin hemichannels (Huang et al., 2007; Romanov et al., 2007). We hypothesize that Type II (receptor) and possibly Type III (presynaptic) cells release ATP at sites containing pannexin/connexin hemichannels. In this study, we examine the presence …
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Identificaiton of the pore-lining helices of connexin 43 gap-junctional hemichannels
… adjacent cells. The hemichannels are hexamers of connexins, proteins that have four transmembrane helices. The transmembrane helices that line the gap-junctional pore have not been identified, and their identification was the main goal of my dissertation project. To accomplish this goal, I used a …
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The Expression Of Connexin-43 By CD11c+ Dendritic Cells Is Required to Maintain CD4+ Foxp3+ Regulatory T Cell Population in Peripheral Lymphoid Organs
… we show that in addition to MHC/TCR interaction, Connexin-43 (Cx43) expression by dendritic cells is required to maintain the TR cell population. CD11c<sup>+</sup> dendritic cells represent a major subset of antigen presenting cells. Using flow cytometry, we have observed that mice which lack Cx43 …
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Regulation of specific connexins differentially alters gap junction permeability and endothelial cell function
… have analyzed the role of specific gap junction connexin proteins in regulating cellular communication and wound healing. Using RNA interference or peptide inhibitors to downregulate specific connexins we examined the role of gap junctions in intercellular diffusion, calcium excitation, and in …
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Aberrant Communication and Protein Kinase C in Experimental Autoimmune Thyroiditis
… (EAT) in Lewis rats revealed a deficiency in connexin expression, connexin assembly and dye transfer [(results corroborated in murine model of autoimmune thyroiditis) (AT)]. Removal of inflammatory mediators from cultures did not reestablish dye transfer despite restoration of intimate cell …
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The impact of reduced neuronal p75NTR expression on sensory neuron phenotype and associated glia
… fibrillary acidic protein (GFAP), SGC p75NTR, connexin 43, we intrathecally infused p75NTR anti-sense oligonucleotides (AS OGN), previously shown by Obata et al. (2006) to effectively suppress p75NTR expression in intact neurons. Male, Wistar rats were divided into three groups, receiving …
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The Role of Cell Communication and 3D Cell-Matrix Environment in a Stem Cell-Based Tissue Engineering Strategy for Bone Repair
… there is an interconnected relationship between connexin-containing gap junctions, cadherins, and integrins in cell communication and the ability of cells to respond to mechanical stimulation. The specific aim of this project was to determine the role of connexin containing gap junctions and …
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Post-Transcriptional Regulation by microRNAS in Pregnancy and Parturition
… genes, oxytocin receptor and connexin-43 and blocked oxytocin-induced contractility in human myometrial cells. Together, these findings implicate the miR-200 family and their targets ZEB1 and ZEB2 as novel P4/PR-mediated regulators of uterine quiescence and contractility during …
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A translational approach to understanding cellular responses to vascular injury
… maintaining open arteries after vascular injury. Connexin 43 is a channel protein that facilitates cell to cell communication and regulates cell proliferation as well as migration, yet it's role in vascular cell types is poorly understood. Here, I hypothesized that Cx43 and its functional …
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Intimations on the Development of Effective Treatment for the Prevention of Preterm Birth: β3 Adrenergic Receptor Signaling in the Human Myometrium
… regulation of the contractile associated protein connexin 43 (Cx43). The mechanosensitive channel Piezo1 was identified in the myometrium and showed to promote relaxation using similar mechanisms involving NO and BKCa. Our studies showed that NO mediated S-nitrosation of Cx43 promote a hemichannel …
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Functional study of Gap Junctional Intercellular Communication in Human Keratinocyte Cell Line
… among almost all tissues, are composed of connexins in vertebrate. The essential role of gap junction-mediated intercellular communications(GJIC) for coordination of cell homeostasis, tissue functions and organ development are well reported by many researches using genetics and mutant …
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Submembrane cytoskeleton-regulated assembly and functional activity of gap junctions
Connexine formen gap junctions, die den Transfer von Ionen, Metaboliten und second messengers zwischen sich berührenden Zellen vermitteln. An vielen Funktionen der Connexine, z.B. zellulärer Transport, Plaquebildung und -stabilität sowie Kanalleitfähigkeit sind Proteine beteiligt, die an die …
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An in Vitro Study of Cellular Cardiomyoplasty: Structural and Functional Interactions of Non-cardiomyocytes and Cardiomyocytes
… cell-cell contact interface. After assessing connexin 43 and N-cadherin expression, higher chances for functional coupling with host cardiomyocytes exist for mesenchymal stem cells (MSC), followed by skeletal myoblasts (SKM), and finally cardiac fibroblasts (CF). Second, we studied the effect …
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Functions of connexin 46 in lens and solid tumors during hypoxia
… are many types and families of protein pores, connexins comprise a unique family. Six connexin monomers assemble into a hemichannel, which is transported to the cell membrane. An opposing cell membrane containing compatible connexin hemichannels is located and connected, forming an …
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Diseased Thyrocytes Have Aberrant Communication and TNF Receptor Secretion
… in MRL-lpr/lpr mice. We have found aberrant connexin expression in thyroid tissue from these mice. The abnormal gap junction assembly persisted when the cells were removed from the inflammation site and cultured <em>in vitro</em>. Primary thyrocyte cultures from these mice were also …
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Role of Astrocyte Network in Edema after Juvenile Traumatic Brain Injury
… in the edema process: Aquaporin 4 (AQP4) and Connexin 43 (Cx43). AQP4 is expressed extensively in astrocyte endfeet, which surrounds the blood vessels as part of the blood brain barrier (BBB). Cx43 is central in astrocyte to astrocyte connection and communication. We hypothesized that AQP4 …
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Mechanisms of Astrocyte Contribution to Bortezomib-Induced Peripheral Neuropathy
… glial activation, and 3) quantified changes in connexin 43, GLT-1, and GLAST to assess whether astrocytic glutamate transport may be altered in BIPN. The results observed in the first aim were that the rat BIPN model is characterized by selective mechanical hypersensitivity and a significant …
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Regulation of Connexin43 by Phosphorylation in Heart Development
… communication through gap junctions formed by connexin 43 (Cx43) (Godwin et al.7 1993). The Cx43 protein expression levels are unclear and what role phosphorylation plays in the regulation of signal transduction through these gap junctions during heart formation. Reportedly, mutations which …
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Primary culture of human limbal-corneal epithelial cells under different conditions
… The expression of differentiation markers CK12, connexin 43 decreased in dHAM cultures, possibly indicating maintenance of the stem cell population. We demonstrate the outgrowths from human cadaveric limbal explants, in six different culture systems, show a steady increase in a wide range of stem …
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