Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 77 for “"Connexin"”.
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Connexin-32 and Connexin-43 Immunoreactivity in Rodent Taste Buds
… cells via specific hemichannels such as pannexin/connexin hemichannels (Huang et al., 2007; Romanov et al., 2007). We hypothesize that Type II (receptor) and possibly Type III (presynaptic) cells release ATP at sites containing pannexin/connexin hemichannels. In this study, we examine the presence …
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Sensitivity to Dopamine D1/D2 Receptor Stimulation in Mice Lacking Connexin-32 or Connexin-36
… interact to produce stereotyped motor activity. Connexin-32 (Cx32) and connexin-36 (Cx36) were targeted for study due to their abundance in neural tissues and presence in the striatum. Mice lacking either the Cx32 or Cx36 gene and their respective wildtype littermates were compared on a climbing …
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Dysregulation of connexin-43 (cx43) in doxorubicin-induced cardiotoxicity
… and dysfunction of gap junctions called connexins in the heart. Here, we examine the effects of the anthracycline DOX on the expression and localisation of connexin-43 (Cx43) in rodent and human cardiac cells and cells of the vasculature to identify if drug treatment impacts this …
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Turnover Rate of The Neuronal Connexin Cx36 In Hela Cells
… on changes in phosphorylation state of the connexin. However, gap junction turnover may also be important for regulating cell-cell communication, and turnover rates of Cx36 have not been studied. Connexins have relatively fast turnover rates, with short half-lives measured to be 1.5 to 3.5 …
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Functions of connexin 46 in lens and solid tumors during hypoxia
… are many types and families of protein pores, connexins comprise a unique family. Six connexin monomers assemble into a hemichannel, which is transported to the cell membrane. An opposing cell membrane containing compatible connexin hemichannels is located and connected, forming an …
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Identificaiton of the pore-lining helices of connexin 43 gap-junctional hemichannels
… adjacent cells. The hemichannels are hexamers of connexins, proteins that have four transmembrane helices. The transmembrane helices that line the gap-junctional pore have not been identified, and their identification was the main goal of my dissertation project. To accomplish this goal, I used a …
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Two-Color Fluorescent Analysis of Connexin 36 Turnover and Trafficking – Relationship to Functional Plasticity
… plasticity on several time scales. Changes in connexin phosphorylation modify coupling in minutes through an order of magnitude, but recent studies also imply involvement of connexin turnover in regulating cell-cell communication. We utilized Cx36 with an internal HaloTag to study Cx36 turnover …
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Modulation of the vascular function by cannabinoids, calcitonin gene related peptide and connexin hemichannels
… (CGRP)-triggered vasodilation and the role of connexin channels in the modulation of blood vessel tone. Additionally, we investigated connexin channels as a putative target to protect blood vessels against cell death associated with stressful conditions like cryopreservation. Blood vessel tonus …
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Effects of connexin modulators in animal models of retinal degeneration: delivery and treatment efficacy
… in AMD progression. It has also been found that connexin hemichannels play a role in the disease‟s pathology. In the normal eye, hemichannels are in a closed state or docked together forming gap junctions for intercellular communication. However, there is undocking of hemichannels and abnormal …
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Function Study of Atrial Fibrillation-Linked Connexin Mutants and Heterotypic Docking Compatibility of Cardiac Connexins
Gap junctions are critical for the propagation of action potentials in the heart. Many autosomal dominant Cx40 mutations have been linked to atrial fibrillation (AF) and have showed GJ impairments. However, others such as L221I and V85I showed apparently no GJ impairments. Cells expressing L221I or …
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Effects of constitutive and acute Connexin 36 deficiency on brain-wide susceptibility to PTZ-induced neuronal hyperactivity
Connexins are transmembrane proteins that form hemichannels allowing the exchange of molecules between the extracellular space and the cell interior. Two hemichannels from adjacent cells dock and form a continuous gap junction pore, thereby permitting direct intercellular communication. Connexin 36 …
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Regulation of specific connexins differentially alters gap junction permeability and endothelial cell function
… have analyzed the role of specific gap junction connexin proteins in regulating cellular communication and wound healing. Using RNA interference or peptide inhibitors to downregulate specific connexins we examined the role of gap junctions in intercellular diffusion, calcium excitation, and in …
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Single channel studies on the modulation of connexin hemichannels by Cx43 targeting peptides and cytoplasmic Ca2+ in the context of heart disease
In vertebrates, connexin hemichannels in the plasma membrane dock head-to-head with their counterparts in adjacent cells to form gap junction channels, allowing direct cell-cell transfer of electric and chemical/metabolic signals. Each hemichannel comprises a hexamer of connexin proteins which …
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The Expression Of Connexin-43 By CD11c+ Dendritic Cells Is Required to Maintain CD4+ Foxp3+ Regulatory T Cell Population in Peripheral Lymphoid Organs
… we show that in addition to MHC/TCR interaction, Connexin-43 (Cx43) expression by dendritic cells is required to maintain the TR cell population. CD11c<sup>+</sup> dendritic cells represent a major subset of antigen presenting cells. Using flow cytometry, we have observed that mice which lack Cx43 …
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Aberrant Communication and Protein Kinase C in Experimental Autoimmune Thyroiditis
… (EAT) in Lewis rats revealed a deficiency in connexin expression, connexin assembly and dye transfer [(results corroborated in murine model of autoimmune thyroiditis) (AT)]. Removal of inflammatory mediators from cultures did not reestablish dye transfer despite restoration of intimate cell …
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Functional study of Gap Junctional Intercellular Communication in Human Keratinocyte Cell Line
… among almost all tissues, are composed of connexins in vertebrate. The essential role of gap junction-mediated intercellular communications(GJIC) for coordination of cell homeostasis, tissue functions and organ development are well reported by many researches using genetics and mutant …
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The Role of Cell Communication and 3D Cell-Matrix Environment in a Stem Cell-Based Tissue Engineering Strategy for Bone Repair
… there is an interconnected relationship between connexin-containing gap junctions, cadherins, and integrins in cell communication and the ability of cells to respond to mechanical stimulation. The specific aim of this project was to determine the role of connexin containing gap junctions and …
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Cardiovascular Characterization of Cx40/Panx1 Single and Double Knockout Mice
Connexins (Cxs) and pannexins (Panxs) are protein families that form large-pore channels which exist at the plasma membrane for both intracellular and extracellular signaling. Given their potential for overlapping cellular signaling functions we proposed that mice lacking both a connexin and a …
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Signaling Mechanisms That Control Gap Junctional Coupling Between Retinal Neurons
… structural substrate for electrical synapses. Connexin 36 (Cx36, and its non-mammalian ortholog connexin 35) is the major neuronal gap junction protein in the central nervous system (CNS), and contributes to several important neuronal functions including neuronal synchronization, signal …
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