Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 62 for “"Confocal microscope"”.
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Microelectromechanical handheld laser-scanning confocal microscope: application to breast cancer imaging
… handheld instrumentation for laser-scanning confocal microscopy (LSCM) and its applicability to breast cancer detection and subsequent image-guided management. LSCM allows high-resolution mapping of spatial variations in refractive index or tumor marker expression within a single cell layer …
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Application of Femtosecond lasers in confocal and scanning tunnelling microscopy
… the use of a Ti:sapphire ultrafast laser with a confocal microscope to precisely induce DNA damage in the nuclei of live cells by multi-photon absorption, the development and comparison of foci counting algorithms for the quantitative assessment of radiation damage and work towards the …
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Computational image analysis of subcellular dynamics in time-lapse fluorescence microscopy
… a different cell line, under both widefield and confocal microscope conditions.
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Nano-optical studies of superconducting nanowire single-photon detectors
… coupling. Nano-optical techniques such as confocal microscopy can be used to image localised areas of SNSPDs providing a direct measurement of the device uniformity. The work in this thesis describes both initial nano-optical testing at visible wavelengths in liquid helium and the …
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DEVELOPMENT OF NON-CONTACT MICROSPHERE CONFOCAL SCANNING MICROSCOPE
The confocal scanning microscope has proven to be a powerful tool to produce high-quality three-dimensional (3D) imaging. However, the resolution of the confocal laser scanning microscope in the visible spectrum is restricted to be ~180 nm and ~500 nm in the lateral and axial direction respectively …
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Indium arsenide quantum dots for single photons in the communications band
… process of a cryogenic scanning laser confocal microscope. Then, the quantum dot growth process is presented along with the results of measurements on early quantum dot samples, which suggested that the initial growth process needed to be refined. We present efforts towards improving …
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Osteogenic and chondrogenic differentiation of rat bone marrow stromal cells in 3D culture with novel PCL-TCP scaffolds
… and distribution were examined by phase-contrast microscope, confocal microscope, MTT assay, and SEM. Osteogenic differentiation was substantiated by Von Kossa staining, heightened expression of ALP and osteocalcin; chondrogenic differentiation was confirmed by alcian blue staining and toluidine …
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ANALYSIS OF TUMOR SPECIFIC PROTEIN EXPRESSION IN GLIOBLASTOMA MULTIFORME (GBMs) TUMORS THROUGH IMMUNOHISTOCHEMISTRY
… with a fluorescent antibody and imaged with a confocal microscope. The tissue images were then analyzed using Imaris software. It was shown that there was a significant difference between the presence of the IDH1 mutation and Basgin, and also MGMT among all of the tissue blocks.</p>
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Investigation of a model FRET system with multiple donors and acceptors. Allowing validation of data interpretation for studies of cells
… are utilised. Cho cells are imaged using a confocal microscope and the plasmid sequences providing them with fluorescence through YFP and CFP are established to be correct. In the pursuit of fine tuning the microscope system to allow the live cells images to be taken and any FRET recognised, …
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Custom built atomic force microscope for nitrogen-vacancy diamond magnetometry
… cantilevers can be used. The AFM and the confocal microscope objective can be locked in position while a piezoelectric stage allows raster scanning of the substrate.
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Diffractive optics for maskless lithography and imaging
… system can be converted into a parallel-confocal microscope, which enables fast, high-resolution imaging. This thesis addresses the performance of diffractive lenses, particularly high-numerical aperture zone plates for lithography and imaging using a combination of experimental and …
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Conjugation of Anti-HER2 Monoclonal Antibody onto a PLGA-PEG Nanoparticle Using CuAAC Click Chemistry
… express the HER2 antigen, are examined with the confocal microscope and the flow cytometer. The results show that the antibody-particle conjugates specifically target only cells that exhibit the HER2 antigen. A series of images taken by a confocal microscope display the internalization of …
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Single-Shot Optical Sectioning Using Polarised Illumination Coded Structured Illumination Microscopy (picoSIM)
The conventional epi-uorescent wide-eld microscope features a uniform illumination of an extended sample region. A problem arises with this setup since light from outof-focus uorophores is also detected. The result is poor quality in the nal image, as out-of-focus structures appear blurred; …
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Palladium, yttrium, copper ternary alloys for hydrogen purification
… hardness measurements, and the use of a confocal microscope and then tested using a hydrogen flow rig, to determine their suitability as a hydrogen purification material. Results showed that the addition of a small amount of copper to a Pd-8at%Y alloy leads to marked changes in a number …
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Optical and spin properties of nitrogen vacancy centers in bulk and nanocrystalline diamond
… In my time in the group, I focused on building a microscope and studying NV centers in bulk and nanocrystalline diamond. I began by building a confocal microscope which was capable of observing and determine single NV centers. I made measurements on the photon statistics of different defects to …
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Homing of 111In-Labeled Bone Marrow Mesenchymal Stem Cells in Acute Brain Trauma Model
… to the traumatic brain was evaluated using confocal microscope. The labeling efficiency of 111In-BMSCs was 66 ± 5%, and their retention rate was 85.3% at 1 h after labeling. There was no difference in the number of viable cells between 111In-BMSCs and controls at 48 h after labeling. …
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Development of a device for multi-modal mechanical manipulation of cells in 2D and 3D engineering environment
… manner to enable the device to be mountable on a microscope for live cell imaging. The Carl Zeiss LSM510 Confocal Microscope was the microscope available for the experimentation. In this project, live cell imaging is only possible with tensile strain. Thus, the tension system was the predominant …
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Morphological Study of Dendritic Spines
… to develop an algorithm to analyse the stack of confocal microscopy images that have been previously manually processed, in order to obtain a precise three dimensional reconstruction of the dendritic spines and study their morphology. The outline of this thesis is as follows. In the first chapter …
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