Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"Confocal laser scanning microscopy (CLSM)"”.
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Test Development and Material Characterization of Hot Poured Crack Sealant at Low Temperature
… of the sealants were also investigated by confocal laser scanning microscopy (CLSM), environmental scanning electron microscopy (ESEM), gel-permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), and thermo-gravimetric analysis (TGA). These techniques reveal the …
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Novel Endodontic Disinfection Approach Using Nanotechnology
… with currently used chemical modalities using confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) as analytical tools. We hypothesized that iron oxide NP with enzyme-like (peroxidase) activity catalyzes H2O2 to promote bacterial killing within dentinal tubules (DT) …
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Microbiological Study of Bacterial Load Reduction in the Root Canal System Utilizing a Novel ErYagLaser
… to evaluate the effectiveness of a novel Er:Yag laser-activated irrigation technique in reducing bacterial load in root canals. In this study, mandibular molars are prepared and cultured with Enterococcus faecalis, and experiment was perform to test whether laser-activated irrigation could …
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Material, acoustic, and mechanical properties of mosquito and midge antennae
… of antennal hearing in insects. To this end, Confocal Laser Scanning Microscopy (CLSM), Finite Element Modelling (FEM) and Laser Doppler Vibrometry (LDV) were employed. The combination of the first two allows, in absence of prior knowledge of the material properties of mosquito or midge …
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Confocal Laser Scanning Microscopy As A Tool For The Investigation Of Tetracycline Fluorescence In Archaeologicalhuman Bone
… of remains postmortem. Standard epifluorescence microscopy is the most common tool employed in the analysis of these labels. Though valuable, this technique is limited by its inability to penetrate bone three-dimensionally and its inclusion of out-of-focus light, possibly disrupting accurate …
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Nanoscale surface modification of wood veneers for adhesion
… ionic strength for polyelectrolyte adsorption. Confocal laser scanning microscopy (CLSM) and Environmental Scanning Electron Microscope (ESEM) were used to characterize adsorbed LbL multilayers of poly(acrylic) acid (PAA) and poly(allylamine hydrochloride) (PAH). Cross-linking between PAA and …
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Evolution and Anatomy of Early Terrestrial Arthropods: Insights from the Rhynie Chert
… took their first steps. This thesis employs confocal laser scanning microscopy (CLSM) to investigate the anatomical and functional adaptations of Rhynie arthropods, addressing key questions about the evolution of arthropod terrestrialisation and the morphological innovations that enabled life …
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Foaming properties of whey protein isolate and [lambda]-carrageenan mixed systems
… while foam microstructures were visualized by confocal laser scanning microscopy (CLSM). For native WPI and [lambda]C mixed system, [lambda]C was needed in order for 5% WPI to form measurable foams. In all three WPI- [lambda]C systems at neutral pH, increasing [lambda]C concentration led to …
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Membrane Surface Engineering for Biochemical Applications
… thus reducing fouling by the aggregates. Confocal laser scanning microscopy (CLSM) is a powerful technique to locate the foulants inside the wet membrane in order to understand more about the behavior of fouling in microfiltration. </p> <p>Membrane surface modification is used to impart …
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Role of Extracellular Polymeric Substances (EPS) on Biofilm Disinfection in a Model Drinking Water Distribution System
… The biofilm structure characterization using confocal laser scanning microscopy (CLSM) revealed that EPS production affected biofilm structure, specifically surface roughness, surface area to volume ratio and average diffusion distance. These structural characteristics were closely related to …
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A New Interfacial Cross Linking Technique: Preparation, Characterization and Evaluation of Calcium alginate Nanoparticles as a Protein Delivery System
… dynamic light scattering, transmission electron microscopy, zeta potential, and differential scanning calorimetry. In vitro drug release studies showed that 90% of entrapped BSA was released within first 8 hrs followed by a slow release pattern. All entrapped protein was released within 30 hours. …
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Studies on selected physicochemical properties and microstructure of β-casein-enriched ingredients for applications in formulated nutritional products
… covalent and generic labelling techniques with confocal laser scanning microscopy (CLSM) allowed simultaneous visualisation of β-CN and WPI at the interface of oil droplets. β-CN-enriched model IF were also produced with β-CNen and WPI at various whey protein:casein ratios in the range …
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Probing the distribution of gliadin proteins in dough and baked bread samples using conjugated quantum dots as a labeling tool
… cyclohexane-1-carboxylat) cross linker. Confocal laser scanning microscopy (CLSM) was used to image QDs conjugated with gliadin antibodies. The mean intensity of gliadin attached to quantum dots for each sample was calculated and plotted. CLSM images showed significant changes in the …
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Introduction of a thermo-sensitive non-polar species into polyelectrolyte multilayer capsules for drug delivery
… the LCST decreases. This work shows Differential Scanning Calorimetry (DSC) and Confocal Laser Scanning Microscopy (CLSM) evidence that the LCST of the PNIPAM can be tuned with salt type and concentration. Microcapsules were used to encapsulate this thermo-sensitive polymer, resulting in a …
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Investigation of Biofilm Development by Antibiotic Resistant Urinary Tract Infection Pathogens and Evaluation of the Antimicrobial Activity of Natural Products on Carbapenem-Resistant Escherichia coli IMP and Klebsiella pneumoniae NDM-1.
… before and after treatment were assessed via confocal laser scanning microscopy (CLSM). Methods This study investigates the effects of the biofilm characteristics of CRE pathogens under different treatment conditions. First, the amount of biofilm formed was evaluated by measurement of TCP at …
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Multilayer capsules with stimuli-sensitive properties : pH-response and carbohydrate-sensing
… of PAH/PMA and P4VP/PMA capsules was studied by confocal laser scanning microscopy (CLSM). They were stable over a wide pH-range and exhibited a pronounced swelling at the edges of stability, which was attributed to uncompensated positive or negative charges within the multilayers. The swollen …
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Synthesis, characterization, and biological evaluation of gelatin-based scaffolds
… (µCT, porosity: 65±11–73±14 vol.-%), and scanning electron microscopy (SEM, pore size: 117±28–166±32 µm). Subsequently, the work focused on the characterization of the gelatin scaffolds in conditions relevant to biomedical applications. Scaffolds showed high water uptake (H: 630-1680 …
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Modelling Nuclear Body Dynamics in Living Cells by 4-D Microscopy, Image Analysis and Simulation
… and analysis of fluorescence images from 4-D confocal laser scanning microscopy (CLSM). These synthetic nuclear bodies are observed in cells transfected with labelled nuclear-targeted Xenopus laevis vimentin. Analysis shows that VNBs undergo anomalous diffusion in the nuclei, independent of …
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Fabrication of Polymersomes using Microfluidic Devices
… characterized by dynamic light scattering (DLS), confocal laser scanning microscopy (CLSM) and cryo transmission electron microscopy (cryo-TEM). Two distinct approaches to control the vesicular self-assembly of copolymer molecules into polymersomes were studied: the undirected self-assembly using …
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The Use of Metal and Metal Oxide Nanoparticles Against Biofilms
… biofilms. Atomic Absorption Spectroscopy (AAS), Scanning Electron Microscopy (SEM) and Confocal Laser Scanning Microscopy (CLSM) was used to further assess the antimicrobial ability of the metal and metal oxide NPs. The prevention of P. aeruginosa and S. aureus adherence on Ag and ZnO thin film …
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