Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"Confocal fluorescence microscopy"”.
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Photophysics of Phenylene Vinylene Polymers and Oligomers: The Influence of Nanocrystalline Domains
… transient emission spectroscopy, streak camera, fluorescence bleach-recovery measurements and confocal fluorescence microscopy. Strong temperature dependence of the fluorescence spectral shape is attributed to the existence of nanocrystalline domains in PPV and an exciton vibrational coupling …
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Functional electrospun fibers for responsive coatings and sacrificial templates
… of fibers and coating include SEM, TEM, confocal fluorescence microscopy, and FTIR. A self-healing system containing amine and epoxy chemistry is also developed and some advantages and limitations of electrospinning are discussed. Development of new coatings synthesized by core-shell …
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Analyzing the interactions of thermoresponsive coacervate-forming biodegradable polyester encapsulation on model protein structure and activity
… enzyme. Encapsulation was visualized using confocal fluorescence microscopy and by labeling β-galactosidase with fluorescein isothiocyanate. Interactions between <sup>15</sup>N-isotopically labelled human ubiquitin c and TR-PEs were investigated through variable temperature nuclear magnetic …
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Régulation du récepteur aux opioïdes de type delta par les protéines Homer1
… membrane and in intracellular compartments by confocal fluorescence microscopy. Lastly, stimulation of hDOP with SNC80 and [D-Ala2] Deltorphine II did not show any significant Homer1 regulatory effect on ERK1/2 signaling pathway. Altogether, these results will further our understanding of the …
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Biomedical ultrasonics, cavitation, and sonoporation
… was verified using high-speed photography and confocal fluorescence microscopy. If drug and genes can be successfully coupled to acoustically active vehicles, sonoporation might revolutionise non-invasive therapy as we know it.
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New chemical and biosynthetic methodologies for the study of lanthipeptides
… their cellular localization was visualized by confocal fluorescence microscopy as a means to determine their modes of action.
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The Development of Peptide Analogues as Potential Fluorescent and PET Imaging Probes.
… focuses on the development of novel dual PET/fluorescence imaging agents based on peptides as targeting entities. Porphyrins are suitable for multimodality imaging applications due to their innate characteristics such as an ability to fluoresce and to coordinate metals. A …
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Unraveling the role of the antifungal peptide AnAFP in Aspergillus niger through a systems biology perspective
… of AnAFP -eGFP was explored using live-cell confocal fluorescence microscopy, revealing compartmentalized translation of the fusion protein, which accumulates in the cell periphery and coincides with a sudden reduction in the hyphal diameter. Lastly, the transcriptomic response of …
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Design, Synthesis, and Characterization of a Novel Class of Mitochondrial Delivery Vectors: Mitochondria-penetrating Peptides
… and monitoring the intracellular localization by confocal fluorescence microscopy, the requisite thresholds for achieving mitochondrial accumulation with a cationic peptide were elucidated. These systematic studies led to the development of a novel class of cationic yet lipophilic peptides, …
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Role of new virulence mechanisms/factors (type 3 secretion system and TOX-R regulated lipoprotein [TAGA]) in the pathogenesis of the emerging human pathogen Aeromonas hydrophila
… the lysis that occurs in the presence of serum. Confocal fluorescence microscopy revealed that the serpin C1-INH binds to TagA on the surface of the bacteria.
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In-operando techniques for early-stage corrosion
… Several complementary approaches were developed: confocal fluorescence microscopy, synchrotron X-ray absorption and diffraction, and electron microscopy. These techniques address various aspects of the corrosion process – mineralogy, oxidation state, domain structure, etc, and provide crucial …
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Identification of Cathepsin B and L as Novel Uva Targets Upstream of Cutaneous Lysosomal-Autophagic Dysregulation
… colocalizing with lysosomes was observed by confocal fluorescence microscopy, and extensive deposition of lipofuscin was detectable by transmission electron microscopy (TEM). Lysosomal expansion was further evidenced by increased immunodetection of lysosomal associated membrane protein-1 …
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PREPARATION AND EVALUATION OF MESOPOROUS SILICA NANOPARTICLES FOR PLATINUM-ACRIDINE ANTICANCER DRUG DELIVERY
… diameter can be generated (transmission electron microscopy, TEM). In these materials, pores as large as 7.2 nm in diameter are formed, based on small-angle X-ray scattering (SAXS) and nitrogen physisorption measurements. These as-synthesized MSNs were further modified with covalently grafted …
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The synthesis and characterisation of inorganic and organic luminophores suitable for biomolecule conjugation
… throughout the cell’s organelles. Whereas, using confocal fluorescence microscopy, the parent complexes were shown not to internalise within the cellular structures. The inherent properties of the dyes, such as Raman and lifetime sensitivity, may then be used to determine pH and oxygen levels …
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Design and applications of 4-yl-(methyl)coumarins and their derivatives
… as secondary ion mass spectrometry (SIMS) and fluorescence microscopy. Chemical gradients were successfully patterned and characterized. The patterned gradients were then used to produce pressure sensitive microfluidic gates based on the variation in surface hydrophobicity. Correlation of mask …
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Development and characterization of anti-EGFR protein-drug conjugates for tumor therapy
… chemical approaches resulted in high background fluorescence. <br />Moreover, new enzymatic strategies were explored for the generation of ADCs. A putative MibH-like peptidyl halogenase was identified by phylogenetic relationship analysis. Also, expression of the human lysyl oxidase-like 2 …
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Marcatura di molecole biologiche a funzione antigenica per lo studio e la caratterizzazione di protocolli di vaccinoterapia in oncologia medica
… localization into DCs, by cytofluorimetric and fluorescence microscopy analysis, respectively. iDC pulsed with 100μg of IgG-FITC/106 cells were monitored from 2 to 22 hours after loading. By the cytofluorimetric analysis it was observed that the monoclonal antibody was completely captured after …
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Investigating the role of zoledronate for inhibition of osteosarcoma metastasis
… blot analysis; 2) cytoskeletal structure with confocal fluorescence microscopy; 3) migration by qualitative and quantitative analyses; and 4) metastases development ex vivo and in vivo using an experimental lung metastasis model. The results of this study were that exposure to ZOL decreased …
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Degradable polymeric nano-films and particles as delivery platforms for vaccines and immunotherapeutics
… films are preserved during release. Using confocal fluorescence microscopy and an in vivo murine ear skin model, we demonstrated delivery of OVA from LbL films into barrier-disrupted skin, uptake of the protein by skin-resident antigen-presenting cells (Langerhans cells), and transport of …
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Exploring the lifecycle of artificial intelligence – deep learning for 1D to 4D biomedical data
… of 3D neutrophil cell nuclei shapes using confocal fluorescence microscopy. This chapter provides insights into the cellular morphology, employing feature extraction, dimensionality reduction, and clustering methods to understand the variations in neutrophil shapes. The analysis underscores …
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