Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 81 for “"Confocal Imaging"”.
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Diffusive Acoustic Confocal Imaging System (DACI): a novel method for prostate cancer diagnosis
… undertaken to develop a diffusive acoustic confocal imaging system (DACI) that aims to differentiate between healthy and the diseased tissues in the prostate. Speed of sound is chosen as the tool to quantify the alterations in the tissues’ mechanical properties at different pathological …
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Scattering from imperfect interfaces
… coefficients for the plane wave case and for the confocal transducer case where these coefficients appear as a sum of convolutions between a source parameter and the crack opening displacements at the imperfect interface. The expressions for the measurement model of the confocal imaging system …
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Cannabinoid CB1 Receptor Expression and Localization in the Dorsal Horn of Male and Female Human and Rat Spinal Cord Tissue
… in humans. We used immunohistochemistry and confocal imaging to investigate the differential expression of the cannabinoid type 1 receptor (CB1R) across dorsal horn laminae and cell populations in male and female adult rats and humans. Qualitatively, we observed increased neuropil …
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Use of electromagnetic stimulation on an Enterococcus faecalis biofilm in root canal treated teeth in vitro
… One sample per group was scored and split for confocal imaging. Null Hypothesis: Teeth treated with EMS in combination with 1.5-percent sodium hypochlorite or 0.9-percent saline will not demonstrate a significant anti-biofilm effect in comparison to those treated with 6.0-percent sodium …
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Investigation of peptide-lipid interaction by fluorescence correlation spectroscopy
… spectroscopy (FCS) and laser scanning confocal imaging. A similar mechanism of MV4s was observed as compared to V4. MV4s induced lipid aggregation before they disrupted the lipid membranes. By comparing between different MV4s, we found that a) highly positively charged peptides …
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Control of flow and oxygen in a 3-D perfused micro-environment fosters balanced survival of hepatocyte-non-parenchymal cell co-cultures
… pneumatic pumps and microfluidics. Staining and confocal imaging of endpoint tissue showed that lower flow rates closer to physiological regimes allowed the survival of SE-1+ SECs, regardless of exogenously added growth factors in the medium. Higher flow rates, exogenous growth factors, and …
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The role of Atgolgin-84A at the plant ER-Golgi interface
… expressed in Nicotiana tabacum and imaged by confocal microscopy with Airyscan detector. High-resolution confocal imaging was used to resolve the Golgi cisternae and the ER-Golgi interface. The data presented here shows that Atgolgin-84A seems to be localised at a pre-cis-Golgi compartment …
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Development of microfluidic devices to maintain and interrogate tissue biopsies - biomedical applications
… Biochemical assays, histological examination, confocal imaging, epi-fluorescent microscopy were all performed to assess the viability of the tissue before and after interrogation by several apoptosis inducing and chemotherapeutic agents. Liver tissue samples were also disaggregated in situ …
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Rational Design of DNA-Based Lipid Membrane Pores
… lipid membrane. With atomic force microscopy, confocal imaging and ionic current recordings we characterise our DNA nanochannels that mimic their natural protein-based counterparts in form and function. We find that they exhibit voltage-dependent conductance states. Amongst other architectures, …
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SAMPLS: A prompt engineering approach using Segment-Anything-Model for PLant Science research
… in plant roots. The state-of-the-art method for confocal image analysis called PlantSeg utilized U-Net for cell wall segmentation. U-Net is a neural network model that requires training with a large amount of manually labeled confocal images and lacks generalizability. In this research, we test a …
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ELUCIDATING THE ROLE OF MIR-10A/B IN THE WOUND HEALING PROCESS IN LARVAL ZEBRAFISH
… fixed sample microscopy, and live imaging. Our results show that the experimental introduction of exogenous miR-10 can rescue the regenerative defect caused by morpholino-mediated knockdown. In addition, miR-10 participates in cellular proliferation without altering apoptosis during …
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Localized adsorption of organic additives during copper electrodeposition
The confocal laser scanning microscope (CLSM) was used in situ during electrochemical experiments to track localized fluorescence patterns of adsorbed organic agents and correlate such adsorption with the changes in surface morphology accompanying electrolysis. In solutions of 5 $\mu$M …
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Improvements in fluidic device evaluation using particle image velocimetry
… gained using phase correlations in comparison to confocal microscopy. This work found substantial improvements in error with respect to the ensemble field for phase correlations while only moderate improvements were observed for confocal imaging with standard processing techniques. Secondly, an …
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Investigating neuronal mitochondrial DNA loss in Drosophila melanogaster
… loss inhibits motor neuron activity. Also, using confocal imaging we find that mtDNA loss does not cause neuronal loss or changes in synaptic bouton number during development. However, we observe a significant decrease in the number of mitochondria and the number of pre-synaptic active zones (the …
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Non-viral drug delivery systems for immune modulation
… siRNA. We next used fluorescent nanoparticles as imaging agents to study these siRNA delivery challenges. Cationic polymers were deposited on the surface of fluorescent core-shell silica nanoparticles electrostatically; the resulting particles were complexed with a nucleic acid and delivered to …
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Developmental Patterning and Cell Fate Specification in the Mouse Colon
… changes in development using a combination of confocal imaging and analysis of single-cell RNA sequencing data. In doing so, I identified not only a spatially heterogeneous developmental timeline along the length of the colon but also asynchronous maturation between the epithelial and …
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Cell surface analysis of the basidiomycete yeast cryptococcus neoformans
… tagging protocols, flow cytometry and confocal microscopy. Cell surface carbohydrate was identified by controlled oxidation in association with biotin hydrazide and fluorescein-streptavidin tagging. Marked amounts of carbohydrate were measured and observed on the cell wall surface of …
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Regulation of mGluR7 trafficking by SUMOylatio in neurons
… Using biochemical approaches coupled with confocal imaging, we find that mGluR7 at Lys889, the sole SUMOylation site on the C-terminus of mGluR7 is a target of SUMO conjugation both by SUMO-1and SUMO-2/3 in HEK293T cells. The SUMOylation of mGluR7 is prevented by SUMO-specific isopeptidase …
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A Glomerular Basement Membrane-on-a-Chip Model for Investigating Charge-Selective Transport Across the Human Filtration Barrier
… the charged dextran perfusion. Before perfusion, confocal imaging confirmed continuous monolayers and podocyte–endothelial contact in co-culture. Charge selectivity was assessed by perfusing fluorescent 10 kDa dextrans of defined charge (cationic, anionic, neutral) as individual injections through …
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