Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"Cone photoreceptors"”.
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Synaptojanin1 is involved in endolysosomal trafficking in cone photoreceptors
… vulnerable to defects in protein turnover. Photoreceptors are highly polarized, specialized neurons that have high protein trafficking demands. At one end of the photoreceptor is the outer segment; the site of photon detection and phototransduction. The outer segment requires a constant …
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Optimizing CRISPR/Cas9 Gene Targeting to Investigate Cone Photoreceptor Development in the Chick Retina
… the loss of vision, due to the degeneration of cone photoreceptors in the retina. Thus, the development of methods to regenerate these cone photoreceptors is an active area of study. In order to find ways to regenerate cones, the molecular pathways and gene regulatory networks that a retinal …
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Efficient In Vitro Development of Photoreceptors from Human Pluripotent Stem Cells
<p>Degeneration of the rod and cone photoreceptors in the human retina is among the most common causes of blindness. Replacing these damaged photoreceptors may help to restore vision. Repairing the damaged retina relies on the insertion of new, healthy cells. Embryonic stem (ES) cells and induced …
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The Isolation of Human Rod and Cone Photoreceptor Activity combining Electroretinography and Silent Substitution Techniques
… to independently assess the function of rod and cone photoreceptors within the human retina. The work in this thesis sought to investigate an alternative method of recording the ERG, using the silent substitution paradigm (Estevez and Spekreijse 1982). The aims are separated into two parts, …
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The Neurogenic Capacity of Müller Glia and Synaptic Remodeling in the Mammalian Retina: Implications for Regenerative Therapies
… Furthermore, survival of small numbers of cone photoreceptors preserved second-order neuronal circuitry locally. This study suggests that the inner retina may remain receptive to interventions even at late stages of disease, and that early cellular transplantation may help prevent or slow …
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Regulation of the catalytic and allosteric properties of photoreceptor phosphodiesterase (PDE6) by the glutamic acid-rich protein-2 (GARP2)
… and adaptation to light stimulation in rod and cone photoreceptors. The central hypothesis of this thesis is that one PDE6 binding partner, the glutamic acid-rich protein (GARP2), may regulate PDE6 to reduce its "dark noise", enhance its sensitivity and conserve metabolic energy during rod …
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A Comprehensive Framework for Adaptive Optics Scanning Light Ophthalmoscope Image Analysis
… (AOSLO), which can visualize human cone and rod photoreceptors in vivo. However, the avalanche of data generated by such imaging systems is often too large, costly, and time consuming to be evaluated manually, making automation necessary. The few currently available automated cone …
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Generation and circuit development of zebrafish retinal horizontal cells
… function to modulate information transfer from photoreceptors. I examined the genesis of HCs using in vivo multiphoton time-lapse microscopy in the zebrafish retina and discovered a novel precursor cell that is dedicated to the generation of HCs. In contrast to the classical germinal cell, the …
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Mechanisms of microglia induced retinal degeneration
… 2) Perturbed multiple retinal neurons including photoreceptors andbipolar cells and 3) Distorted RPE morphology (Chapter 3). <br/>To understand the mechanisms of active microglia-mediated retinal neuronal andRPE damage in the DKO mice, I investigated in Chapter 4 the phenotype andfunction of …
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Decoding the Little Skate Retina: Structural Variants and Developmental Dynamics
… cells express canonical markers of both the cone and rod pathway, suggesting the precursors utilize elements of the cone and rod program to generate their apparent hybrid photoreceptor structures. Analysis of little skate onecut1, a transcription factor gene critical to the development of …
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Decoding the Little Skate Retina: Structural Variants and Developmental Dynamics
… cells express canonical markers of both the cone and rod pathway, suggesting the precursors utilize elements of the cone and rod program to generate their apparent hybrid photoreceptor structures. Analysis of little skate onecut1, a transcription factor gene critical to the development of …
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RATE-LIMITING STEP OF CONE PHOTOTRANSDUCTION RECOVERY AND OGUCHI DISEASE MECHANISMS
<p>ABSTRACT RATE-LIMITING STEP OF CONE PHOTOTRANSDUCTION RECOVERY AND OGUCHI DISEASE MECHANISMS By Frank Sungping Chen Advisor: Ching-Kang Jason Chen, Ph.D. Retinal photoreceptors provide the first gateway in which light information from the environment is transformed into neuronal signals. The …
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The Role of Peripherin in Photoreceptor Outer Segment Morphogenesis
… our eyes. This task is performed by the rod and cone photoreceptors, which both contain a highly evolved sensory cilium called the outer segment. The outer segment is a specialized cellular compartment that contains all of the protein machinery involved in converting the initial light signal into …
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Cloning and functional characterization of novel genes expressed preferentially in the human retina
… of neuronal cell types including rod and cone photoreceptors, bipolar cells, ganglion cells, amacrine cells, horizontal cells and Müller glia. In response to light, a coordinated series of molecular events, the so-called phototransduction cascade, is triggered in photoreceptor cells and …
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Comparing Differentiated Rod And Cone Transcriptomes Reveals Rax As A Controller Of A Defined Cone Gene Regulatory Program
<b>Purpose</b><br></br>Rods and cones convert light stimuli into signaling information for brain- mediated visual perception. Specific gene mutations primarily affecting both photoreceptor (PRs) types lead to retinal dystrophies, including rod-cone (retinitis pigmentosa) and cone-rod degeneration. …
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The use of Silent Substitution in measuring isolated cone- and rod- Human ERGs
… silent substitution paradigm in obtaining cone- and rod- isolating ERGs using sine- and square- wave temporal profiles. The ERGs achieved in this manner were shown to be photoreceptor-selective. Furthermore, these responses did not only provide the functional index of photoreceptors but …
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Cone packing measurements from confocal and split-detector adaptive optics images in human eyes
… imaging has been used to calculate metrics of cone packing in healthy and diseased eyes. However, there is a lack of data comparing metric values obtained using different AOSLO imaging modalities, as well as the impact of different image analysis methods on these metrics. Here, we 1) calculate …