Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 107 for “"Conditioned Media"”.
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Purification and Characterization of Proteoglycan from Bovine Aortic Endothelial Cells Conditioned Media, and its Interaction with Basic Fibroblast Growth Factor (bFGF)
… we have purified proteoglycans from BAE cells conditioned media (BAE PG), and further separated the PG into two fractions, PG-I and PG-II, by ion exchange chromatography on a Q-Sepharose column using a linear salt gradient (0.15 M to 1.2 M). PG-I and PG-II elute at 0.85M salt and 0.1M salt …
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Delineating the metastatic process in osteosarcoma; the role of bone cells, the cell environment and autophagy
… partly due to a paucity of research into mediators of the metastatic process. Recent evidence shows that targeting osteoclasts (OCs) with zoledronic acid (ZA) increases the number of metastatic lung lesions clinically, whereas treatment with Fulvestrant increases OC numbers and decreases …
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Investigating the inhibitory effects of secreted proteins on macrophage in glioblastoma
… to the microenvironment were studied using conditioned media and the day 3 with higher protein concentration were considered for studying the phagocytic activity and antigen presentation marker expression. The proteins present in the conditioned medium which were derived from these …
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Investigating the role of nuclear transport proteins secreted by cancer cells
… lines of cervical cancer and non-cancer origin. Conditioned media collected from control, eGFP-expressing or Kpnb1-eGFP expressing HeLa and CaSki cervical cancer cells was used to treat recipient cells, both cancerous and non-cancerous. This was followed by monitoring the uptake of Kpnb1-eGFP and …
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An investigation of the effects of an antimicrobial peptide on the survival of Acanthamoeba and intracellular bacteria associated with Cystic Fibrosis
… strategies to combat each other. Bacterial conditioned media were tested for amoebicidal and amoebistatic abilities. P. aeruginosa, Str. pneumoniae and H. influenzae conditioned media demonstrated amoebistatic and amoebicidal effects. Acanthamoeba conditioned media exhibited bactericidal …
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Effects of ovarian steroids on bovine mammary epithelial cells: in vitro and in viro evidence of indirect stimulation of proliferation
… of Mac-T cells over serum before treatment. Conditioned media from Mac-T or Fib-T (mammary bovine fibroblast cell line) with or without steroids were tested for ability to increase Mac-T cell proliferation. Growth of Mac-T cells was greatest in Fib-T + E conditioned media followed by Fib-T, …
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The Role of Autotaxin in Cancer
… cancer cells, secrete ATX and its substrate LPC. Conditioned media from 0082T cells increased the growth of both PANC-1 and MIA PaCa-2 cells. Treatment of 0082T CAF cells with IOA-289 during media conditioning reduced PDAC cell growth, without impacting CAF cell numbers. Lipidomic analysis showed …
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Exploring the influence of adipokines on neuronal function in Alzheimer’s disease
… To understand the potential rescue effect of conditioned media from adipocyte-like cells on neuronal-like cells, and commercially available adipokines (chemerin, leptin and resistin) on neuronal-like cells and effect of chemerin on human skin fibroblast cells derived from AD and non-AD donors, …
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Collagen gene expression in human cancer
… lines. The fibroblasts were also incubated with conditioned media prepared from the tumour cells. Collagen production was analysed using the collagenase assay and the results showed that co-cultured tumour cells, as well as growth in the presence of tumour cell conditioned media, resulted in …
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Oncogene-induced reprogramming of heterotypic cellular interactions
… 5 I further examined the effects of KITLG and conditioned media from SW48 cells expressing KRAS G12D. Another cell type expressing KIT are fibroblasts and KITLG induced KIT receptor phosphorylation, activating downstream signalling through the ERK1/2 and AKT pathways. I observed that …
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CHCHD2 and the tumor microenvironment in glioblastoma
… form of primary brain tumor in adults, with a median survival time of only 14.6 months. GBM tumors present with chemo- and radio-resistance and rapid, diffuse invasion, making complete surgical resection impossible and resulting in nearly universal recurrence. While investigating the genomic …
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Pancreatic Cancer: Oncomicrobes, Electric Fields, and Fluid Flow
… (nPECs). This invasion process was partially mediated by the bacterial adhesin Fap2. nucleatum infection induced a distinct cytokine secretion profile, characterized by elevated IL-8, CXCL1, MIP-3α, and GM-CSF. F. nucleatum invasion promoted migration and proliferation in Panc1 and BxPC3 …
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Retinoic Acid Pathway Inhibition to Expand Human Hematopoietic Progenitor Cells with Islet Regenerative Capacity
… in ALDHhi cells compared to untreated cells. Conditioned media from BMS 493-treated cells also increased human ß-cell proliferation in vitro. However, intrapancreatic transplantation of BMS 493-treated cells did not reduce hyperglycemia in STZ-treated NOD/SCID mice. Further characterization of …
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Tracing of Alpha-to-Beta Cell Transition Following Treatment with CHIR-Injected Human Multipotent Stromal Cell Secreted Proteins
… bioengineered via WNT pathway activation (CHIR+ conditioned media; CM), secrete factors that promote beta-cell recovery in streptozotocin (STZ) treated NOD/SCID mice. Here, we investigated whether this regeneration process involves alpha-to-beta cell conversion, using a …
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Effects of light-emitting diode photostimulation on burn wound healing
… 730nm, 880nm, or combination-670nm/730nm/880nm. Conditioned media were analyzed for VEGF and NO. Excised burn wounds underwent measurement of surface area, tensile strength, VEGF, nitrites, and immunohistochemical markers (iNOS, VEGF, cyclooxygenase-2, Factor VIII, ED-1) on days 3, 7, and …
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Examining the binding of humanin and acetylcholinesterase with amyloid beta
… between Aβ and HN. When using lung cancer cells conditioned media, we saw more HN was bound to Aβ after ATP addition, while bind of AChE to Aβ was diminished by addition of ATP</p>
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Inflammatory Breast Cancer: The Immune Perspective
… known about the role of inflammation or immune mediators in IBC. Therefore, we analyzed blood samples from IBC patients and non-IBC patients, as well as healthy donor controls to establish an IBC-specific profile of peripheral blood leukocyte phenotype and function of T cells and dendritic cells …
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Developing a model to assess the contribution of cytokeratin 19-expressing cells during multipotent stromal cell-induced islet regeneration
… multipotent stromal cells (hMSC) generated conditioned media (CM) that promoted β-cell regeneration in streptozotocin-treated mice. Ductal-derived endocrine progenitors, which have been shown to generate β-cells following pancreatic injury, represent a candidate for the ‘signal-receiving …
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Cytokine and neurotransmitter regulation of glial cell production of Il-6; indications for neurodegenerative disorders
… tumor cells. The release of IL-6 in the C6 cell conditioned media was quantified using the IL-6 dependent 7TD1 hybridoma bioassay; We decided to investigate GABA ability to inhibit the release of IL-6 by IL-1beta; To better understand the signal pathways of IL-1beta that results in the release of …
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