Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"Conditional knockout mouse"”.
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Defining the Role of Pannexin1 in Skeletal Muscle and Satellite Cell Function in vivo and ex vivo Using a Novel Conditional Knockout Mouse Model
… Using a novel skeletal-muscle-specific Panx1 knockout (Panx1^smKO) mouse model, this thesis highlights the influences Panx1 plays in muscular development and maintenance, as well as in muscle function. Loss of Panx1 led to a fiber-type switch coinciding with smaller fibers in both the tibialis …
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The Role of Cleavage and Polyadenylation Spcific Factor 6 (CPSF6) in Hematopoiesis
… impacts on this process. To test this, we used conditional knockout mouse models to examine the function of <em>Cpsf6</em> in both embryonic and adult hematopoiesis. Our findings demonstrate that <em>Cpsf6</em> is essential for embryonic hematopoiesis but is dispensable for maintaining adult …
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Investigating The Role of The P63 Isoform Δnp63 In Lung Stem Cell Populations and Lung Cancer
… remains unclear. We utilized a ΔNp63-specific conditional knockout mouse model to determine ΔNp63’s role in lung progenitor cells and lung cancer. In vivo and in vitro experiments revealed a role for ΔNp63 in maintaining lung progenitor cells. ChIP-seq results indicate that deletion of ΔNp63 …
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Characterization of Cre Mouse Models to Target CNS Barriers for Generating Conditional Knockouts of ABC Transporters
… and have been studied using global transporter knockout mouse models. Since the CNS compartment and location of transporters is unique at each barrier site, a global knockout model does not address questions of how each barrier site contributes to ineffectiveness of drugs getting into the CNS. …
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The role of Hedgehog signalling in T cell effector function
… CD4+ T helper cell polarisation. Using conditional knockout mouse models and clinically-approved small-molecule inhibitors, this work shows that the canonical Hh pathway acts transcriptionally through Gli3 and metabolically through AMPK to control Th polarisation in vitro and in vivo. …
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Regulation of synaptic function and plasticity by cyclin-dependent kinase 5
… Cdk5 ablation. From multiple Cdk5 conditional knockout mouse models, we determined that Cdk5 is essential for memory formation and synaptic plasticity. Loss of Cdk5 in the hippocampus disrupts the cAMP pathway due to increased phosphodiesterase proteins. This dysregulation of cAMP …
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Deciphering The Roles of Δnp63 In Regulating Epithelial to Mesenchymal Transition, Cancer Progression and Metastasis
… and homeostasis. Utilizing a <em>ΔNp6</em>3-conditional knockout mouse model generated in the Flores lab, I demonstrated that the loss of ∆Np63 in basal epidermal cells elicits an “irreversible” epithelial to mesenchymal transition (EMT) via activation of EMT-associated transcription factors, …
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CROSSTALK BETWEEN PRC1 AND PRC2 IN INTESTINAL LINEAGE COMMITMENT AND CELL IDENTITY
… vivo context. Taking advantage of Cre-dependent conditional knockout mouse models targeting distinct Polycomb activities in the intestinal epithelium, the present work proposes to characterize the interplay among the PRC1 subcomplexes and their relationship with PRC2 activity. In particular, it …
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ROLES OF HAND2 TRANSCRIPTION FACTOR IN UTERINE RECEPTIVITY AND DECIDUALIZATION
… of a transcription factor, HAND2, in sensitizing mouse uterus for implantation and decidualization. In mouse, HAND2 expression was localized mainly in the endometrial stromal cells even before the blastocyst implantation. The expression of HAND2 increased after blastocyst implantation and …
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Role of transcription factor Runx1 in uterine stromal cell differentiation and maternal-fetal interaction during mouse pregnancy
… uterine stromal cells during decidualization. Conditional deletion of the uterine Runx1 gene led to severe embryo growth retardation and pregnancy loss during mid-gestation. Histological analysis of the Runx1-null uteri at gestation days 10-12 revealed an abnormally dense decidual tissue …
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Analysis of the Role of the Transcription Factor C/EBPbeta in Controlling Uterine Functions During Early Pregnancy
… the uterus. The development of a C/EBPbeta-null mouse model, which is infertile, presented us with an opportunity to analyze the role of this molecule in uterine function. We discovered that C/EBPbeta functions in two distinct manners: (i) by acting as a mediator of E-induced proliferation of the …
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Analysis of the role of the transcription factor C/EBPβ in controlling uterine functions during early pregnancy
… in the uterus. The development of a C/EBPb-null mouse model, which is infertile, presented us with an opportunity to analyze the role of this molecule in uterine function. We discovered that C/EBPb functions in two distinct manners: (i) by acting as a mediator of E-induced proliferation of the …
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Characterization of the TCOF1 Gene Using a Neuroblastoma Cell Line and a Mouse Model
… mice. In this study, we generated Tcofl conditional allele mice with loxP sites flanking exon 1. These mice were crossed with Wntl-Cre transgenic mice to generate a conditional knockout of Tcofl specifically in neural crest (NC) cells. Homozygous conditional knockout mice show …
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Evaluation of Sex Differences in the Hippocampus and Pituitary of Egr1 conditional knockout mice mediated by Nestin-Cre
… gene and Nestin-Cre as a system for generating conditional knockout mouse strains. The Chapter begins with the identification of Egr1 gene and its protein structure, then proceeds to grasp its link to memory with behavior testing. The critical role of Egr1 in the pituitary and what cell …
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The role of ERα in the uterus during embryo implantation and in the ovary during ovarian tumorigenesis
… to placentation. Through the development of knockout mouse models, it is known that ERalpha, the predominant estrogen receptor in the uterus, is essential for the embryo to attach to these cells prior to invasion. However, we have uncovered for the first time the essential role of ERalpha in …
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The role of Cdc7 and cyclin-dependent kinases in DNA replication and S phase
… the design strategy for generating a Cdc7-conditional knockout mouse (cko) is outlined and results from the screen for a transgenic founder are presented. A Cdc7-cko mouse will be a valuable tool to further dissect Cdc7 function and regulation in mammalian cells. In the final section, S …
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The role of non-genetic variability in Acute Myeloid Leukaemia
… (Tzelepis et al., 2016). More recently, using a conditional knockout mouse model for Kat2a our lab showed that it sustains KMT2A/MLLT3 AML stem cells. Kat2a is a classical regulator of transcriptional variability, its loss leading to cell-to-cell heterogeneity in transcription levels, including …
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Investigating The Roles of Tap63 and Tap73 In Cutaneous Squamous Cell Carcinoma and Lung Adenocarcinoma
… in the skin, I have also developed a novel TAp73 conditional knockout mouse, which includes a conditional fluorescent reporter (TAp73fltd) that allows us to follow the deletion event at the cellular level. Using this model we have found that the loss of TAp73 accelerates the initiation and …
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Left, Right, and Center: A Novel Midline Barrier Separates Asymmetric Extracellular Matrix During Intestinal Development
… and preliminary experiments with conditional knockout mouse lines can inform future experiments to understand how versican is implicated in vascular and intestinal smooth muscle development. Although the right and left DM asymmetrically express many genes, including some which …
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The Role of Id Proteins in the Development and Function of T and B Lymphocytes
… to control gamma delta T cell development. Id3 knockout mice demonstrate a dramatic expansion of innate-like Vgamma1.1<super>+</super> Vdelta6.3<super>+</super> T cells in the neonatal stage, suggesting that Id3 is an inhibitor of their development. Interestingly, Id3 knockout mice with a B6/129 …
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