Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"Condensin"”.
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Analyse von Condensin-Komplexen in Drosophila melanogaster Charakterisierung der Untereinheit CapG und Identifizierung von Interaktionen
… Stabilität der Zellen. Der heteropentamere Condensin-Komplex ist hierbei an der Strukturierung der Chromosomen und deren physikalischer Stabilität während der Segregation maßgeblich beteiligt. In höheren Eukaryoten konnten zwei dieser Komplexe (Condensin I und Condensin II) identifiziert …
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The role of the chromosomal passenger complex and condensin complex in meiotic chromosome cohesion and segregation
The canonical mitotic cell cycle is modified in metazoans to achieve a variety of developmental goals. Among these, meiotic divisions reduce the DNA content of the cell, haploid gametes fuse and reenter mitotic cycling, and mitoses of early embryogenesis, in many animals, employ a rapid cell cycle …
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Dissecting The Roles of Human Smc Complexes In Transcription Regulation and Chromatin Organization
… SMC complexes including Cohesin complex and Condensin complex has been widely proposed to organize 3D genome structure, and further regulate metazoans’ gene transcription. Here, we aim to dissect the direct functions of SMC complexes (both Cohesin and Condensin) in transcriptional regulation …
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Linker Histone Medicated Regulation of Mitotic Chromosome Compaction and Individualization
… segregation. Recent studies have shown how condensins and DNA topoisomerase II organize the mitotic chromosome. However, the regulation of these factors in maintaining proper chromosome size in different cell types remains a mystery. Here, I investigated the role of the linker histone …
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Genome organization by loop extrusion
… by loop extrusion. We also find that cohesin and condensin, two deeply conserved SMC complexes, play strikingly different roles in the yeast mitosis. While cohesin is responsible for compacting the bulk of chromosome arms, condensin has a more targeted role compacting the rDNA proximal regions and …
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Functional investigation of the role of the retinoblastoma protein in genome stability
… also investigates the functions of the pRB-condensin II complex in maintaining genome stability, specifically in interphase cells. Using a gene-targeted mouse model that disrupts the ability of pRB to recruit condensin II, Rb1L, locations of pRB-dependent condensin II recruitment were …
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Mechanisms for maintaining genomic integrity during chromosome segregation in budding yeast
… monopolin (made up of Lrs4, Csml and Maml) and condensin, a protein complex required for chromosome condensation, and their roles in chromosome segregation during mitosis and meiosis. During mitosis, Lrs4-Csml and condensiin reside in the nucleolus where they regulate the maintenance and …
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Chromosome dynamics during cell divisions in Drosophila melanogaster: The role of Rad21 in meiotic cohesion and dynamic analysis of the condensin subunit CapG in early embryonic mitotic divisions
… require multisubunit protein complexes, the condensin and the cohesin complexes, respectively. Both complexes are composed of two core SMC subunits and a set of non-SMC subunits, which are conserved among most eukaryotes. In the first part of my thesis, I have analyzed the localization and …
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Characterizing the contribution of the LXCXE binding cleft to pRB-mediated genome stability and tumor suppression
… genome stability suggests that a pRB-E2F1-Condensin II complex localizes to pericentromeric heterochromatin. In the absence of this complex, DNA double strand breaks are observed and persist into mitosis and the ensuing G1 phase of the cell division cycle. Moreover, haploinsufficiency of …
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Identification and Characterization of Hells-CDCA7, a Nucleosome Remodeling Complex Required for Mitotic Chromosome Structure
… including histones, topoisomerase II and condensin are known to compact and shape the chromatin into rod-like chromatids. However, a complete picture of the proteins involved in shaping mitotic chromatin is unsettled. Here, I perform mass spectrometry on chromatin isolated from Xenopus egg …
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Quantitative Analysis of the Structural Dynamics of Mitotic Chromosomes in Live Mammalian Cells
… This axial shortening was not affected in condensin-depleted cells, and was independent of the poleward pulling motion on kinetochores, but it nevertheless depended on dynamic microtubules. Perturbation of this shortening caused a severe phenotype of multi-lobulated daughter nuclei, …
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Dynamic Simulation of active/inactive Chromatin Domains
… structures. The idea is that basically every condensing agent (HMG/SAR, HP1, cohesin, condensin, DNA-DNA interaction...) can be modelled as an effective attractive potential of specific chain segments. This way the formation of individual 1Mbp sized rosettes from a linear chain could be …
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Patterns and processes driving chromosome organization
… of DT-40 chicken cells and uncover the role of Condensin in disassembling interphase chromatin structure during prophase. We develop a model for prophase condensation and explore different interactions between loop extruding Cohesins and Condensins. We find that non-trivial interactions between …
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Molecular bases underlying chromosome fragility at Replication Slow Zones in Saccharomyces cerevisiae
… conserved type II topoisomerase and condensin complex. Finally, factors involved in maintaining the stability of RSZs were also explored. The Rrm3 helicase and Psy2 phosphatase complex were found to suppress chromosome breakage at RSZs in a manner dependent on Tel1, another checkpoint …
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Transcriptional variation in muscle from cattle with alternative NCAPG/LCORL QTL genotypes
The NCAPG/LCORL locus is a major QTL on bovine chromosome 6 (BTA6) that influences growth and composition in cattle. Genetic variation at this locus is associated with alterations in the developmental program of individuals throughout life that may be detected through transcriptional variation. …