Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"ConA"”.
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Die Bedeutung zytotoxischer Effektormoleküle bei der ConA induzierten Hepatitis
Die ConA induzierte Hepatitis in der Maus dient als Modell humaner Leberentzündungen und erlaubt detaillierte Analysen zur Rolle immunkompetenter Zellen und und ihrer Effektormoleküle bei den auftretenden pathologischen Prozessen. Frühere Studien haben gezeigt, daß ZTL (zytotoxische T-Lymphozyten) …
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Control of covalent and non-covalent presentation of biomolecules within collagen GAG scaffolds
… attachment of biotinylated Concanavalin A (ConA) via two distinct covalent patterning techniques: 1-ethyl-3-3-dimethylaminopropylcarbodiimide hydrochloride (EDC) crosslinking and benzophenone (BP) biophotolithography. The results indicated that longer contact time of ConA with the CG …
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Can levamisole upregulate the equine cell mediated immune response in vitro?
… or with one of the mitogens: concanavalin A (ConA) or phorbol 12-myristate 13-acetate and ionomycin (PMA/I), or with a combination of the above mitogens and levamisole at several conditions. Cellular proliferation was assessed using a colorimetric bromodeoxyuridine ELISA assay. The second aim …
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Fluorescence Resonance Energy Transfer (FRET) Based Sensors for Bioanalysis
… with Fluorescein tagged Concanavalin A (FITC-ConA). It was found that carbohydrates and glycoproteins inhibit the binding between dextran-TR and FITC-ConA labeled particles. The inhibition effect was concentration dependent thus enabled screening carbohydrates and glycoproteins based on their …
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Síntesis y reactividad de sistemas de 1,3-tiazolio-4-olatos derivados de carbohidratos
… y de tiofeno. La reacción de estos compuestos cona zodicarboxilato de dietilo da lugar a anillos de triazina y anillos de tiazol. Los nuevos heterociclos mesoiónicos permiten sintetizar C-ni-cleósidos policíclicos y anillos de 1,2 dihidrotiofenos cuando reaccionan con dipolarófilos olefínicos …
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The Effect of Dietary Fat Level on Tumor Development and Immunity in BALB/C Mice Fed Casein of Wheat Gluten as Protein and Challenged with Herpes Type 2-transformed Cells
… using phytohemagglutinin (PHA), concanavalin A (ConA) or lipopolysaccharide (LPS) as mitogens.</p> <p>The type of protein present in the diets of the mice (casein or wheat gluten) appeared to be the most important factor influencing tumor development. Mice fed the 11% casein had the lowest tumor …
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Host-Parasite Interrelations: Bacterial Adherence to and Invasion of Epithelial Cells
… wheat germ agglutinin (WGA) and concanavalin A (ConA). Glycoproteins (ovomucoid, fetuin, mucin, ovalbumin) inhibited binding by P.IIa, WGA, and ConA to variable degrees. HeLa cell glycopeptides, generated by pronase digestion of chloroform:methanol-extracted cells, were tested for their ability …
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Modulation of Single-Walled Carbon Nanotube Fluorescence via Enzyme Reaction, Controlled Aggregation and Hydrogel Swelling Towards a Continuous Glucose Sensor
… affinity based sensor. Addition of concavalin A (ConA) causes the dextran coated nanotubes to aggregate resulting in SWNT PL intensity diminution, while subsequent additions of glucose cause the ConA-SWNT aggregates to dissolve and the PL to recover. Finally, we demonstrate hydrogel swelling as a …
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Neue fluoreszierende Copolymere für sensitive Detektionssysteme in Wasser
… ß Cyclo¬dextrin (ß CD) bzw. ConcanavalinA (ConA) wurde als Erkennungsstruktur an einem Fluoreszenzlöscher immobilisiert. Polymer-basierte Fluoreszenzsensoren sind in der Fachliteratur gut dokumentiert. In der Regel sind Signalgeber und Analyt jedoch statistisch im Polymer verteilt, da sie …
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Development of an Encapsulation Strategy for an Implantable Optical Glucose Sensing Technology Compatible with a Self-Cleaning Membrane
… competitive binding assay using Concanavalin A (ConA) as a component in a Förster resonance energy transfer (FRET) approach is being developed for encapsulation within the thermoresponsive hydrogel. In this research, a double network nanocomposite PNIPAAm (DNNC) hydrogel’s glucose diffusion, …
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SERS-based nanoparticle biodetection using carbohydrate-lectin interactions
… surface. This resulted in the production of glyconanoparticles with unique surface functionality, for example, glucose or galactose. Additionally, functionalising the particles with Raman reporter molecules (RRMs) resulted in the measurement of surface enhanced Raman scattering (SERS) signals. …
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Response of Peripheral Blood Lymphocytes from RAO-affected Horses to b2-Agonist Stimulation
… horses. Isolated PBLs were activated with ConA and stimulated with salbutamol. Response to agonist binding was indirectly determined using flow cytometric methodology and verified by Western blot. Activated PBLs from RAO horses demonstrated a significant response to β2-agonist binding …
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Investigation into reliability and performance of an implantable closed-loop insulin delivery device
… for FITC-insulin in the non-polymerised dex2M-conA gel (NP gel) determined using mathematical models, QSS and TL slope methods was 1.05 ± 0.02 x 10-11 m2/s and in the cross-linked dex500MA-conAMA gel (CL gel) was 0.75 ± 0.06 x 10-11 m2/s. In response to physiologically relevant glucose triggers …
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The immunotoxic effects of aldicarb
… in the SMLR and AlloMLR or when stimulated with ConA or anti-CD3 mAbs, in the presence of normal macrophages, demonstrated normal responses. In contrast, normal T cells exhibited decreased responsiveness in the presence of aldicarb-treated macrophages. The fact that aldicarb did not affect the T …
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Collective Chemotaxis of Retinal Neural Cells from Drosophila Melanogaster in Controlled Microenvironments
… The μOS was coated with Concanavalin A (ConA), seeded with 15 dissociated brain complexes from third instar larvae, the prime retina development phase for Drosophila. A fibroblast growth factor-8 (FGF-8) gradient (100ng/mL) was applied and images of the cell migration were taken every 30 …
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Μορφογένεση της επιδερμίδας : μελέτη της υδατανθρακικής συστάσεως των κυτταροπλασματικών μεμβρανών
… βιοτινυλιωμένων λεκτινών (PNA, DBA, WGA, RCA, ConA και UEA), η αντιπαραβολή των ευρημάτων με τα αντίστοιχα της διεθνούς βιβλιογραφίας και με εκείνα της υγιούς επιδερμίδας του ενηλίκου και τέλος η διερεύνηση του ενδεχομένου μελλοντικής αξιοποιήσεως των ευρημάτων της μελέτης στα πλαίσια …
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Expression And Characterization Of Mycobacterium Paratuberculosis 19kda With Posttranslational Modification
… not be a glycoprotein when failed to react with ConA stain. The 16/19 kDa forms of the protein were evaluated against T-lymphocytes from Crohn's disease patients and normal controls. T- proliferation assay included controls such as PHA and PPD from M. paratuberculosis. There was not a significant …
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Late glacial to Holocene climate and vegetation changes on the Tibetan Plateau inferred from fossil pollen records in lacustrine sediments
… of sediment core recovered from Lake Donggi Cona (in the north-eastern part of the TP) which has been dated back to the Last Glacial Maximum (LGM). A new data-set of modern pollen collected from large lakes in arid to semi-arid regions of central Asia is also presented herein. The concept of …
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Avaliação do éster de acridina como marcador conjugado à lectina em histoquímica
Transformações neoplásicas apresentam modificações na composição e distribuição de oligossacarídeos da superfície celular de glicoproteínas e glicolipídeos, a partir desta característica, lectinas vêm sendo utilizadas como sondas no auxílio de diagnósticos histopatológicos de tecidos de mama, útero …
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REGULATION OF GLYCOSYLATION IN MAMMALIAN CELL LINES: INSIGHTS FROM STRUCTURAL GLYCOMICS, ANALYSIS OF GLYCOSYLATION-RELATED GENES AND FUNCTIONAL RNAI SCREENS
… control the cell surface glycosylation. Using ConA and AAL lectins to specifically label oligomannose-type and fucosylated glycans at the cell surface, the primary screening results suggested a significant number of genes may play a role in the transition of oligomannose/hybrid-type glycans to …
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