Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"Complexin"”.
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Structural Studies of Complexin/SNARE Interactions
… was to delineate interactions between complexin and SNARE components that lead to the formation of a primed minimal fusion machinery. We have generated and used new constructs of short forms of synaptobrevin and complexin, as well as constructs of SNAP-25 and syntaxin that have …
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Expression and Function of Complexin 1 in Mammalian Sperm Acrosomal Exocytosis
… complex, whose core includes three proteins. Complexins 1 and 2 are highly related cytosolic proteins that bind tightly to the assembled SNARE complex and promote neuronal exocytosis. Studies of complexin 1 and 2 deficient mice suggest that they serve redundant functions in cells expressing …
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Structure and Function of Proteins Involved in Regulated Secretion: Synaptotagmins and Complexin
… regulators of neurotransmitter release such as Complexin, and Synaptotagmin as well as other required proteins such as Munc13 and Munc18. Complexin is a small soluble protein that binds to the SNARE complex with high affinity and regulates the formation of the SNARE complex. Synaptotagmin is the …
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A genetic analysis of complexin function in neurotransmitter release and synaptic plasticity
… signal is still unclear. Here we show that complexin, a small alpha-helical protein that binds fully assembled SNARE complexes, functions as the synaptic vesicle fusion clamp in vivo. Drosophila has a single complexin homolog that is abundantly expressed in presynaptic nerve terminals. …
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Competition Between Synaptotagmin 1 and Complexin for SNARE Complex Binding, Controls Fast Synaptic Vesicle Exocytosis
… 1, fast Ca2+- triggered exocytosis requires complexins. Synaptotagmin 1 and complexins both bind to assembled SNARE complexes, but it is unclear how their functions are coupled. To clarify previous debates on calcium dependent and independent binding between synaptotagmin 1 and SNARE …
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Alternative splicing and RNA editing of the Complexin C-terminus regulates neurotransmitter release in Drosophila
… learning and memory. Functional regulation of Complexin (Cpx) provides an entry point for altering presynaptic output since the protein controls spontaneous and evoked neurotransmitter release through its effects on SNARE complex assembly. The Drosophila cpx locus undergoes alternative splicing …
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Synaptic structure and function at the Drosophila larval neuromuscular junction : a molecular analysis of complexin and radish
… (Syt1), and there is growing evidence that Complexin (Cpx), which binds to the SNARE complex with high affinity and 1:1 stoichiometry, can act as a vesicle fusion clamp. As suggested by its name, however, Cpx appears to play a more complex role in vesicle release, carrying out different …
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Dissecting Roles for the Macromolecular Machinery Involved in Neurotransmitter Release
… by preventing trans-SNARE complex disassembly. Complexin-1 also impaired de-priming, while synaptotagmin-1 may have assisted in priming and hindered de-priming. Additionally, I used various biophysical approaches including ITC and NMR to shed light into how Complexin has dual roles in fusion. …
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Unraveling the Role of SNARE Interactions in Neurotransmitter Release
… of the core complex may mediate membrane fusion. Complexin is a highly conserved cytoplasmic protein that binds tightly to the SNARE complex. Analysis of the interaction between complexin and the SNARE complex showed that complexin binds to the groove between the synaptobrevin and syntaxin …
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Molecular Mechanisms Controlling Synaptic Vesicle Fusion
… otherwise. In synapses, this protein is called Complexin and a second protein, called Synaptotagmin, releases the clamp upon a rapid influx of calcium, the hallmark of an action potential. How Complexin clamps is a subject of great interest in the field, and an area of active research. What is …
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Regulation of SNARE-Mediated Synaptic Vesicle Release by Synaptotagmins and Complexins
… Among these proteins are synaptotagmins and complexins, two protein families that directly interact with the SNARE complex, and that are interdependent to each other in regulating SNARE-mediated synaptic vesicle release: complexin clamps neurotransmitter release until synaptotagmin is …
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Characterization of Synaptotagmin Function In Calcium Dependent Neuronal Exocytosis
… SNARE-Komplex ist jedoch weitgehend ungeklärt. Complexine bilden eine weitere Familie von Proteinen, die mit dem neuronalen SNARE-Komplex interagieren and an der Regulation der kalziumabhängigen Exozytose beteiligt sind. Das Zusammenspiel von Complexin und Synaptotagmin ist weitgehend …
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Molecular Interactions During Fertilization: Sperm -Egg Binding and Regulation of the Acrosome Reaction
… membrane fusion were identified in sperm. Complexin is present in the acrosomal region and an antibody to complexin blocked the acrosome reaction, suggesting complexin may regulate membrane fusion during the acrosome reaction. Munc18, a molecule known to regulate membrane fusion in neurons, …
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Single-molecule fluorescence resonance energy transfer study of SNARE-mediated membrane fusion
… many accessory proteins, such as synaptotagmin, complexin and Munc18. By a new lipid mixing method at the single-vesicle level, we are able to accurately detect different stages of SNARE-mediated membrane fusion including docking, hemi and full fusion via FRET value of single donor/acceptor …
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Biophysical Characterization of SNARE Complex Disassembly Catalyzed by NSF and alphaSNAP
… robust ist.Das SNARE-Komplex-bindende Protein Complexin1 inhibierte die Reaktion in Abhaengigkeit von der alphaSNAP-Konzentration. Die Ergebnisse zeigen, dass Complexin und alphaSNAP unter in vitro-Bedingungen miteinander um die SNARE-Komplex- Bindung konkurrieren. Der inhibitorische Effekt von …
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Neue Einblicke in die SNARE-vermittelte Fusion: Detektion einzelner Proteoliposomen mit einem konfokalen Mikroskop
… Regulators der neuronalen Exozytose, des Complexin, auf die Fusion von Liposomen untersucht. Complexin wird als Stabilisator für ein spätes Fusionsintermediat der neuronalen Exozytose, den primed Zustand, diskutiert. Ein solch stabilisierender Effekt konnte mit dem hier entwickelten Assay …
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Molecular Determinants of Synaptic Vesicle Exocytosis and Endocytosis Coupling
… release such as Sr2+ substitution and complexin loss-of-function. The link between asynchronous release and slowed endocytosis was due to selective targeting of fused synaptic vesicles towards slow retrieval by the asynchronous release Ca2+ sensor synaptotagmin7. This divergence in Ca2+ …
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Structural and Functional Studies of C2-Domain Proteins Involved In Neurotransmitter Release
… We also showed that synaptotagmin 1 displaces complexin from the reconstituted SNARE complex in the presence of Ca2+, and that the synaptotagmin 1 C2B domain is primarily responsible for SNARE binding. Moreover, NMR spectroscopy and site-directed mutagenesis studies yielded structural …
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Structural and Functional Studies of the Munc13 MUN Domain and the RIM C2B Domain
… using cofloatation assays suggested both MUN and complexin can compete with Munc18-1 for SNARE complex binding in a membrane environment. Our studies also suggested that the membrane environment can modulate the strength of protein-protein interactions remarkably, which emphasize the importance to …
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Unraveling the Functions of Synaptotagmin and Munc13 in Neurotransmitter Release
… as core components. Munc13, synaptotagmin and complexin underlie the exquisitely tight regulation of synaptic exocytosis. The SNAREs form the SNARE complex that brings synaptic vesicles and plasma membrane together. This complex is disassembled by NSF/α-SNAP (soluble N-ethylmaleimide sensitive …
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