Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"Complementarity-Determining Region (CDR)"”.
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Construction and mutagenesis of an anti-insulin single chain Fv (scFv) using protein engineering techniques: Identification of peptide mimotopes which inhibit scFv-insulin binding.
… that of insulin. The immunoglobulin variable region genes of the anti-insulin antibody were rescued and cloned independently as V(H) and V(k). A single chain Fv (scFv) was constructed using a polymerase chain reaction (PCR) assembly technique from the anti-insulin V(H) and V(k). The scFv …
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In Vitro Evolution of Antibody Affinity using Libraries with Insertions and Deletions
… combining site is the length variation of the complementarity determining region (CDR) loops. Following antigen stimulation, further diversification through the process called somatic hypermutation (SHM) leads to antibodies with improved affinity and specificity. Sequence diversification by SHM …
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Conformational analysis of antibody binding site using EDMA methods /
… between conformational samples of antibody complementarity determining region (CDR) loops, isolated LI loop and LI in three-loop assembly (LI, L3 and H3) obtained from Monte Carlo simulation. After the significant difference is detected, the specific inter-Ca distance which contributes to …
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Kinetics of Disordered Proteins and their Interactions
Disordered proteins and regions are highly prevalent in the human proteome, and are often implicated in disease. However, methods to study these systems in detail are lacking, and the potential for thermodynamic and kinetic characterisation using experimental methods is limited. Molecular …
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Determinants of antibody specificity
… clearance rates can be driven wholly by variable-region mediated effects independent of neonatal Fc receptor (FcRn) binding. Focused on the antibody variable region, we next utilized our nonspecificity assay as a sorting tool to look at a naive repertoire library. We found significant …
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Engineering and characterization of human single-chain T cell receptors
… interact with peptide-MHC (pepMHC) through six complementarity determining region (CDR) loops. The relative positions of these loops is conserved, such that the regions derived from the germline encoded regions of the TCR (i.e. CDR1 and CDR2) are positioned primarily over the MHC helices whereas …
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Effect of disruption of 3D8 complementarity-determining regions on properties of 3D8 antibody
… or deletion of amino acids within or near CDRs (complementarity-determining regions) in antibodies have been utilized to improve antigen-binding affinity and/or stability. However, little is reported about functional consequence by the disruption of whole amino acid sequence in CDRs of an …