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Showing 1 to 3 of 3 for “"Competitive assays"”.

  1. Synthesis and Structure-Activity Relationship Studies of 3-(N-Butylethanimidoyl)-4-hydroxy-2H-chromen-2-one (BHC) Analogues for Myosin II Inhibition

    … of the analogues determined through ATPase assays found the trend that both longer linear alkyl and methyl-aromatic imino tails are favored. The lead analogues tested containing a hexyl and a benzyl imino tail have a IC50 values of 1.2 ± 1.0 µM and 0.8 ± 0.2 µM, respectively, to skeletal …

    unr Repository record for Synthesis and Structure-Activity Relationship Studies of 3-(N-Butylethanimidoyl)-4-hydroxy-2H-chromen-2-one (BHC) Analogues for Myosin II Inhibition (opens in a new tab)

  2. Structural basis for the function of the transcriptional regulator KstR from Mycobacterium tuberculosis

    … quenching assay). Protein-DNA binding assays have demonstrated that the 21-amino-acid extension at the N-terminus of KstR imposes a positive but non-essential role for KstR-DNA interaction. Protein-ligand binding assays and competitive assays (of protein, DNA and ligand) have been used …

    auckland-ms Repository record for Structural basis for the function of the transcriptional regulator KstR from Mycobacterium tuberculosis (opens in a new tab)

  3. Optimization and Applications of Fluorescence anisotropy assays and Fluorescence Resonance Energy Transfer Measurements

    … and negatively charged dyes. Secondly, FA based assays for direct determination of affinities of CaM-target interactions are developed and the results are consistent with previously reported values. Finally, a FRET based methods are used to study the mechanism of activation of PMCA by CaM and …

    ku Repository record for Optimization and Applications of Fluorescence anisotropy assays and Fluorescence Resonance Energy Transfer Measurements (opens in a new tab)