Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"Competitive ELISA"”.
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Antibody-based biosensor assays for the detection of zilpaterol and markers for prostate cancer
… used in the development of immunoassays in a competitive ELISA format and on the Biacore (a surface plasmon resonance-based optical biosensor capable of monitoring biomolecular interactions in 'real-time'). A zilpaterol-HSA conjugate was used to generate and characterise single chain antibody …
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Antibody-Based Detection of 3-Methylglutaconic Acid
… several enzyme-linked immunosorbent assay (ELISA) formats were investigated to find the most sensitive assay. From this study, a competitive ELISA was chosen and optimized. The assay was then tested for reproducibility, specificity, and matrix effects in urine. In seven of 11 assays analyzed …
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INDUCED SPAWNING OF CAPTIVE TRIPLETAIL LOBOTES SURINAMENSIS
… and females during the spawning season using a competitive ELISA assay. Sex identification was most effective using 11-Ketotestosterone with a 1.19% error rate in cross validation of the training set. Males with low levels of this hormone in the test dataset were mis-identified with a higher …
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Evaluating the impact of heat stress and altered glycemic state on plasma ɣ-Aminobutyric Acid (GABA) in lactating Holstein cows
… GABA concentrations were determined using a competitive ELISA. The data were analyzed in two ways. The first analysis included data from all treatment periods and yielded no period-based differences in plasma GABA concentrations. In this analysis, plasma GABA was lowly correlated to plasma …
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Relationship Between Testosterone and Dihydrotestosterone With Microbiota and Toll-Like Receptor Protein Localization in the Male Pig Reproductive Tract Prior to and Post Pubertal Attainment
… concentration of DHT were quantified using a competitive ELISA and plasma T concentrations were determined using chemiluminescence. Data were analyzed using PROC CORR in SAS 9.4. Significant correlations between T concentrations and relative abundance of the phyla Firmicutes, Proteobacteria, …
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The isolation, identification and detection of Clostridium Difficile
… of an enzyme linked immunosorbant assay (ELISA) for detection of Clostridium difficile in broth cultures and faeces, to develop a new selective isolation medium and rapid identification test for Cl.difficile, to investigate the use of SDS-PAGE for typing isolates and to determine the …
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Development of novel Competitive Enzyme-linked immunosorbent assays to detect SARS-CoV-2-specific antibodies in animals
… species. Here, I describe the development of a competitive enzyme-linked immunosorbent assay (cELISA) to detect SARS-CoV-2 antibodies in mammals for which species-specific reagents are not available. Therefore, cELISAs were developed to detect SARS-CoV-2 spike S1 and S2 domains and nucleocapsid …
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INSIGHTS ON THE CROSSTALK BETWEEN THE IMMUNE SYSTEMAND CANCER: IMMUNE CHECKPOINT MOLECULES AND LYMPHOIDPOPULATIONS ASSESSMENT IN CANINE ROUND CELL TUMORS
… protein (sPD-L1) plasmatic concentrations by competitive ELISA in dogs with nodal lymphoma. Their potential prognostic role was also investigated. B-cell lymphomas showed the highest frequency and level of mPD-L1 expression, aggressive T-cell lymphomas exhibited the lowest one, and T-zone …
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Impact of Adjuvants on the Antibody Responses to Pre-pandemic H5N1 Influenza Vaccines
… by binding to H5 antigen microarray and competitive ELISA assays was not different than induced by alum or vaccine alone.</p> <p>Finally, we tested the contribution of the cytosolic innate immune sensing complex known as the NLRP3 (also known as cryopyrin, CIAS1, or Nalp3) inflammasome is …
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Investigating thyroid hormone transport and regulation in chorionic somatomammotropin RNAi models of intrauterine growth restricted pregnancies
… analysis, T4 and T3 levels were assessed using competitive ELISA assays, and DIO2 and DIO3 expression was measured using qRT-PCR. T4 was reduced by 16% (P ≤ 0.05) in CSH RNAi IUGR umbilical vein serum samples compared to control umbilical vein serum samples, and by 29% (P ≤ 0.05) in CSH RNAi …
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VGF: a novel and predictive biomarker of Parkinson’s disease
… analyses performed by immunofluorescence and/or ELISA in brain regions relevant to PD pathology (olfactory bulb and substantia nigra) and in plasma. To investigate the temporal dynamics of VGF processing during disease progression, specific antibodies targeting distinct VGF-derived products were …
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ELUCIDATING DIFFERENT CLOSTRIDIUM PERFRINGENS TYPE D VACCINE PROTOCOLS IN EWES AND LAMBS
… CptD vaccination history in previous years. A competitive ELISA was used to determine serum CptD ETX titers as inhibition %. CptD ETX were measured pre-vaccination, 14-days (-14d), and 7 days (-7d) pre-lambing, and at lambing (d 0). Lambs born to ewes in each trial were vaccinated at 3, 14, or …
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Developability assessments and analytical characterization of recombinant protein antigens formulated as low-cost, multi-dose, aluminum-adjuvanted vaccines
… scanning calorimetry (DSC) and antibody binging (ELISA) in stage 2. Correlations of stage 1 developability data with stage 2 storage stability studies were observed for aluminum-adsorbed P[8] antigens. These results were confirmed using a second NRRV protein antigen, P[4]. This two-stage vaccine …