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Showing 1 to 20 of 82 for “"Comet assay"”.
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Untersuchung des genetischen Schadens in peripheren Lymphozyten von Dialysepatienten mittels Mikrokerntest und Comet-Assay
… genetischen Schaden, ermittelt durch den Comet-Assay und den Mikrokern-Test in peripheren Lymphozyten bei Dialysepatienten bzw. Prädialysepatienten.
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Untersuchungen zur Genotoxizität in Fischen und Fischzellen mit der Einzelzell-Gelelektrophorese (Comet Assay) - Möglichkeiten und Grenzen im Umweltmonitoring -
In den Untersuchungen zum Comet Assay mit Fischen und Fischzellen stand die Etablierung des Systems mit primären Hepatocyten und Kiemenzellen aus dem Zebrabärbing (Danio rerio), der Vergleich der genotoxischen Wirkung nach in vitro- und in vivo-Exposition sowie die Korrelation der Ergebnisse mit …
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Strahlensensibilität von Fibroblasten und Lymphozyten bei Brustkrebspatientinnen: Vergleich des alkalischen Comet Assay mit der klinisch beobachteten Hautreaktion nach Bestrahlung
… dieser Arbeit sollte untersucht werden, ob der Comet Assay als prädiktiver Test zur Erfassung der Strahlenempfindlichkeit normaler Gewebe geeignet ist. Dazu wurden bestrahlte Hautfibroblasten und periphere Blutlymphozyten von 30 Brustkrebspatientinnen im Comet Assay analysiert und die Resultate …
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Reduction of white blood cell DNA strand breaks (alkaline comet assay) in smokers and non smokers by black tea consumption
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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Quantifizierung von DNA-Schäden an adhaerenten Zelllinien nach Bestrahlung mit 188 Re- bzw. Röntgenstrahlung unter Zugabe von Methimazol, Nicotinamid und Perchlorat durch den Comet Assay
Dissertationsschrift zur Erlangung eines doctor medicinae (Dr.med.) der Medizinischen Fakultät Carl Gustav Carus der Technischen Universität Dresden
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Eignung der high throughput Version des Comet Assays als Screening-Verfahren
… zu erfassen. Hierzu wurde der häufig angewandte Comet Assay als high throughput-Verfahren weiterentwickelt. In der vorliegenden Arbeit wurde untersucht, inwieweit sich dieses neue high throughput Verfahren für ein Screening auf Gentoxizität von Umweltproben und Chemikalien während der …
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In vitro chemically-induced DNA damage in cancer patients and healthy individuals. The effect of genotoxic compounds in cells from polyposis coli, colon cancer patients and healthy individuals.
… habits were taken into consideration. The assays used in this study included the Comet assay, the Micronucleus assay, the Micronucleus ¿ FISH assay and the sister chromatid exchange assay. The food mutagens, PhIP and IQ, as well as titanium dioxide nanoparticles (TiO2 NPs) induced a dose …
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Quantifizierung von DNA-Schäden peripherer Lymphozyten bei Patienten mit chronischer Niereninsuffizienz
… spricht. In dieser Arbeit wurde mittels Comet Assay, einem sensiblen Testverfahren zur Quantifizierung von DNA-Schäden auf Einzellzellniveau, aus verschiedenen Gruppen von chronisch Nierenkranken die Zellkern-DNA von peripheren Lymphozyten auf Schäden untersucht. Neben Patienten mit …
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A Novel Drug Delivery System Incorporates a Functional Composite Scaffold Derived from Taxifolin with Potential Cytotoxic and Anticancer Effects on Triple Negative Breast Cancer
… efficacy of the drug post-operatively. The Comet assay was utilised to analyse DNA damage and repair in lymphocytes from breast cancer patients versus healthy controls. CCK-8, qPCR and Western Blot were employed to study viability, gene, and protein expression regulation during taxifolin …
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A Novel Drug Delivery System Incorporates a Functional Composite Scaffold Derived from Taxifolin with Potential Cytotoxic and Anticancer Effects on Triple Negative Breast Cancer
… efficacy of the drug post-operatively. The Comet assay was utilised to analyse DNA damage and repair in lymphocytes from breast cancer patients versus healthy controls. CCK-8, qPCR and Western Blot were employed to study viability, gene, and protein expression regulation during taxifolin …
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Genotoxic effects of oestrogens and nano-NSAIDs: Genotoxic effects of oestrogens in vivo and nano- and bulk forms of NSAIDs on blood samples from prostate cancer patients
… to what extent DNA damage occurred. The alkaline Comet assay was the chosen method used to assess double-strand DNA breakage by examining the Olive tail moment and %age tail DNA. Tissues from the testis, bone marrow, liver and blood were analysed after an 8-day duration of exposure. Statistically …
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A Novel Drug Delivery System Incorporates a Functional Composite Scaffold Derived from Taxifolin with Potential Cytotoxic and Anticancer Effects on Triple Negative Breast Cancer
… efficacy of the drug post-operatively. The Comet assay was utilised to analyse DNA damage and repair in lymphocytes from breast cancer patients versus healthy controls. CCK-8, qPCR and Western Blot were employed to study viability, gene, and protein expression regulation during taxifolin …
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Mammalian cell cultures as a model system for the determination of cytotoxicity induced by cholesterol oxidation products
… biochemical hallmark of apoptosis, therefore the comet assay as a measure of DNA fragmentation was assessed as a measure of apoptosis. The level of DNA fragmentation induced by 7β-OH, as measured using the comet assay, was similar for both cell lines. Therefore, it was concluded that the comet …
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Investigation of the genotoxic potential of the marine biotoxins okadaic acid and azaspiracids
… study, DNA fragmentation was detected using the COMET assay. In contrast to most other available studies, two further endpoints were included. The Trypan Blue Exclusion assay was used to provide information on possible cytotoxicity and assess the right concentration range. Flow cytometer analysis …
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Micropatterned cell arrays for detecting DNA damage
… assessing DNA damage at a global level is the COMET assay, based on electrophoretic extension of the nuclear DNA in single cells embedded in agarose. In this assay, the size and shape of the extended 'comet tail' can be correlated to breaks in the DNA. This assay was first developed in the mid …
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DNA damage and repair during the mammalian hormonal cycle
… including gender, age and diet may influence the comet assay responses in lymphocytes when monitoring human genotoxicity, we posed the question whether the menstrual cycle would influence the comet assay responses in lymphocytes to any significant extent. The study approach was to determine DNA …
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Engineering a single cell microarray platform for high throughput DNA damage and repair analysis
… analysis. The single cell gel electrophoresis (comet) assay has become a standard method for DNA damage analysis, however, it is not well suited for use in clinical and epidemiological settings due to issues of low throughput, poor reproducibility, and a laborious image analysis requirement. To …
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