Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"Colorimetric detection"”.
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Development of stimulus-responsive polydiacetylene systems for molecular colorimetric detection
Direct and convenient detection of hazardous molecules in the environment, agriculture, and industry plays a critical role in humanity's health and safety. In order to achieve this goal, I have rationally designed polydiacetylene systems that possess unique optical properties such as color changing …
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Development of CIELAB colour system for colorimetric detection of heavy metals in wastewater using metal nanoparticles
Thesis submitted in fulfilment of the requirements for the degree of Doctor of Philosophy in Chemistry in the Faculty of Applied Sciences at Durban University of Technology, 2021.
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DEVELOPMENT OF A RAPID DIAGNOSTIC ASSAY FOR THE COLORIMETRIC DETECTION OF LEGIONELLA BACTERIA USING LOOP MEDIATED ISOTHERMAL AMPLIFICATION
… as 10-12 copies of DNA in food or water samples. Colorimetric <em>Legionella </em>LAMP can detect as few as two copies of DNA in under 90 minutes. The colorimetric LAMP assay can be used as a fast and accurate method for detecting <em>Legionella </em>in water samples and for monitoring purposes, …
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EXPLORING THE CORRELATION OF THE PRESENCE OF THE ST3GAL3 GENE IN INDIVIDUALS DIAGNOSED WITH ATTENTION DEFICIT HYPERACTIVITY DISORDER USING LOOP MEDIATED ISOTHERMAL AMPLIFICATION WITH COLORIMETRIC DETECTION
<p>Attention Deficit Hyperactivity Disorder, ADHD, is one of the most common neurodevelopmental disorders in children. Children who have been diagnosed with ADHD will likely have trouble paying attention, controlling compulsive behaviors, and staying within appropriate activity levels. Diagnosis of …
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DEVELOPMENT OF A RAPID DIAGNOSTIC ASSAY FOR THE COLORIMETRIC DETECTION OF BACTERIAL AND VIRAL PATHOGENS USING LOOP-MEDIATED ISOTHERMAL AMPLIFICATION COUPLED WITH A PEPTIDE NUCLEIC ACID AND GOLD NANOPARTICLE REPORTER SYSTEM
<p>Detection of pathogenic agents remains pivotal to the control and prevention of infectious disease. While diagnostic methods continue to break barriers, the need for accurate, sensitive, and rapid methods persists. Current methods for pathogen detection, including culture, immunochemical, or …
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Application of polymerization-based amplification in point-of-care diagnostics
… and easy-to-use with minimal infrastructure. Colorimetric detection methods that provide results that are readable by eye, without reliance on specialized and expensive equipment, have great utility in these settings. Existing colorimetric methods based on enzymatic reactions and gold …
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Paper-based Molecular Technologies for Faster, More Accessible Infectious Disease Diagnostics
… tests made from cellulose paper that use colorimetric readouts—where a visible color appears if disease biomarkers are present—have shown promise in reducing device cost, equipment and training needs. However, their ability to support a variety of diagnostic methods has not been …
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Assessment of Chemiluminescent Detection System for Forensic DNA Restriction Fragment Length Polymorphism Analysis
… was to determine whether a chemiluminescent detection system can give the sensitivity of 32P isotopic detection for human DNA restriction fragment length polymorphism (RFLP) analysis and have the speed, safety, and reduced cost of colorimetric detection. The assessment was made on the basis …
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Triplex dna receptor-gold nanoparticle conjugates for detection of oxidized dna base
… and letting them excrete though urine. Detection of those abnormal nucleobases in urine has been suggested as a convenient way of early cancer diagnosis. 5-OHU (5-hydroxyuracil), the oxidation product of cytosine, is one of those abnormal nucleobases and it potentially forms base pairs …
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Development of Universal Biosensing Platforms Based on CRISPR/Cas12a systems
… and cost-effective method for diverse target detections. This thesis focusses on the development of CRISP/Cas12a based biosensing platforms for nucleic acid and protein detection. Two distinct CRISPR/Cas based diagnostic methods were developed. The first developed method is a plasmonic CRISPR …
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Pump-free magnetophoresis for improved point-of-care diagnostics
… device described in chapter two. The limit of detection was improved by three orders of magnitude and calculated at 4.67 x 102 CFU/mL in pooled human urine, which is below detection limits for commercial urinary tract infection tests. Colorimetric detection was used instead of fluorescence …
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A Rapid Colorimetric Peptide Nucleic Acid Loop-Mediated Isothermal Amplification Assay for the Detection of the IDH1 Mutation in Glioblastoma
… intraoperatively. We designed a novel colorimetric peptide nucleic acid loop-mediated isothermal amplification (PNA-LAMP) method that rapidly detects the <em>IDH1</em> R132H mutation in GBM. PNA-LAMP amplifies target DNA under isothermal conditions with high specificity and speed. The …
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Nanobiotechnology Enabled Environmental Sensing of Water and Wastewater
… caused by such contaminants. Many analytical detection techniques have been developed and applied for environmental sensing. However, these techniques are often reliant upon centralized facilities and require intensive resources. For these reasons their use can be challenging under …
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The Synthesis and Host-Guest Applications of Synthetic Receptor Molecules
… with DNA, and have been developed as a selective colorimetric detection system, that upon binding turns the solution from a red to blue in color.2</p><p>Cyclotriveratrylene (CTV) 1 is a common supramolecular scaffold that has been previously employed in guest-host chemistry, and the construction …
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RAPID COLORIMETRIC IDENTIFICATION OF ESCHERICHIA COLI SHIGA TOXINS 1 AND 2, AS WELL AS EAE AND AGGR, USING LOOP MEDIATED ISOTHERMAL AMPLIFICATION FOLLOWED BY PNA/AUNP DETECTION
… a pH indictor dye. This method allows for easy colorimetric detection of LAMP amplicons. If the target sequence was amplified, hydrogen ion release during phosphodiester bond formation will decrease the pH of the reaction. This causes the pH indicator dye to turn from pink to yellow. There are …
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Colorimetric sensor arrays: development and application to art conservation
… and application of cost-effective, compact colorimetric arrays, focusing specially on their use in the cultural heritage and art conservation communities. One of Society's most important cultural responsibilities is the preservation of the past for the future. The surest way to protect a …
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Industrial applications of flow injection analysis
… by peroxides in organic medium, followed by the colorimetric detection of the latter as the thiocyanate complex. The system exhibits a wide dynamic range and good linearity (e.g., linear r^2, 0.9943 for 0.1 -120 meq/kg cottonseed oil hydroperoxides) with a good throughput rate (up to 60 …
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Detection of Biologically Important Molecules
… has been of great interest. The selective detection of a specific anion is particularly challenging. In the first chapter, I describe the synthesis of a TBS-protected fluorescein dye (1.1) and its use for the selective detection of fluoride. Solutions of 1.1 either in DCM or in aqueous …
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Fluorescent and Colorimetric Biosensors Based on DNAzymes and DNA Aptamers
… hand, technology for sequence-selective DNA detection has advanced remarkably. Two primary examples are molecular beacon-based fluorescent detection and nanoparticle-based colorimetric detection. Therefore, combining the two fields opens new avenues to obtain highly sensitive and selective …
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Manipulating Conjugation in electronic polymers and graphitic materials: chemosensors, precursor routes, and self-assembly
… colored and facilitates spectroscopic and colorimetric detection of CWA mimics in solution and as a thin film. Similarly, we report the incorporation of CWA-responsive units into random copolymers prepared by ringopening metathesis polymerization (ROMP) to create highly modular, chromogenic …
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