Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"Colon Carcinoma"”.
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Nanosecond Pulsed Electric Field Effects on Cell Cycle and Apoptosis
… ultimately to reach an apoptotic threshold for colon carcinoma cells using HM 16p53+/+ and HCT116p53-/- cells. There are three specific aims in this dissertation, (1) determine the effect of nsPEF exposures on colon carcinoma cells in respect to apoptosis, p53 and proliferation at conditions …
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Real-Time Bioluminescence Imaging Of Salmonella-Neoplastic Cell Interactions
… induce pro-inflammatory responses in HCT116 colon carcinoma cells, specifically, NF-κB activation. Then, I performed a high-throughput siRNA screen to identify novel host kinases and phosphatases involved in detection of Salmonellaand activation of NF-κB signaling. For this work, I used a …
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Diverse roles for the mitogen-activated protein kinase ERK2 revealed by high-throughput target identification
… ERK2 substrates in 3T3-L1 fibroblasts and in colon carcinoma cell lines. We investigated one novel ERK2 substrate, the transcriptional repressor ETV3, in detail and found that phosphorylation abrogates binding to DNA by ETV3, and that mutation of key phosphorylated residues to alanine blocks …
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CALCIUM SENSING RECEPTOR FUNCTION IN COLON: A ROBUST PROMOTER OF DIFFERENTIATION AND TUMOR SUPPRESSOR
… of calcium sensing receptor (CaSR) in the human colonic crypt epithelium is linked to cellular differentiation while its lack of expression is associated with undifferentiated and invasive colon carcinoma. Recent studies show that CaSR suppresses the malignant phenotype through a variety of …
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Mechanisms of Cell Death Initiated in Herpes Simplex Virus Thymidine Kinase Expressing Colon Tumor Cells Treated with Ganciclovir and UCN-01
<p>Metastatic colon carcinoma is the second leading cause of death from malignancy in the United States, and development of more effective treatments is essential. Heterologous expression of Herpes Simplex Virus Thymidine Kinase (HSVtk) in combination with the prodrug, ganciclovir (GCV), has shown …
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The Cx3Cl1-Cx3Cr1 Chemokine Axis Contributes to Tumor Immune Evasion and Its Blockade Enhances Responses to Anti-Pd-1 Immunotherapy
… enhances survival in an immunocompetent mouse colon carcinoma model through a decrease in tumor-associated myeloid populations and their conversion into an anti-tumor phenotype.</p>
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Bioengineered Submucosa for Studying Effects of Microenvironmental Mechanics on Colorectal Cancer Cells
… varying matrix alignment and embed an HCT-116, colon carcinoma cell line, spheroid into our organoids to simulate an integrated tumor compartment. Through the modulation of our submucosal model, we determine the effects of varying alignment and stiffness on colorectal cancer cell phenotype. …
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Identification and functional evaluation of a bioactive peptide QUB-1369 Secreted from the skin of The Common Frog (Rana Temporaria)
… assay found a weak inhibition of HCT 116 Human Colon Carcinoma cells at a concentration of 100 µM.
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Quantitative analysis of the receptor-induced apoptotic decision network
… of kinase pathways governing apoptosis in human colon carcinoma cells. Simulations of our fuzzy logic model recapitulated most features of the data and generated several predictions involving pathway crosstalk and regulation. Fuzzy logic models are flexible, able to incorporate qualitative and …
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Catalytic and non-catalytic mechanisms involved in Glutathione S-transferase Pi mediated cytoprotection
… in vitro, where silencing of GSTP1 in a colon carcinoma cell line demonstrated increased levels of glutathione disulphide and protein S-glutathionylation, a reversible post-translational modification involved in thiol protection. Subsequent analysis of GSTP1 silencing in this cell line …
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Microbiota regulate short chain fatty acids and influence histone acylations in intestinal epithelial cells.
… can upregulate histone acylations in both colon carcinoma cells and intestinal organoids in a dynamic manner. In addition, I identified that class I histone deacetylases (HDACs) are efficient histone decrotonylases. I have achieved this through a combination of biological analysis of the …
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Tumor Response TCF-4/β-Catenin Regulatory Elements for Enhancing Cancer Gene Therapies
… are frequently associated with progression of colon carcinoma and most other types of epithelial carcinomas. This usually results in stabilization of β-catenin protein levels, followed by transactivation of Tcf-4/β-catenin responsive genes. The effectiveness of a Tcf-4/β-catenin transcriptional …
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Isolation and effects of citrus limonoids on cytochrome p450 inhibition, apoptotic induction and cytotoxicity on human cancer cells.
… induction on human neuroblastoma (SH-SY5Y) and colonic adenocarcinoma (Caco-2) cells. The first study developed a bulk purification method for limonoids, from seeds and molasses of citrus fruits, using a combination of chromatographic techniques. This also resulted in an efficient purification …
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Regulation of Rad51D In Homologous Recombination Dna Repair by Alternative Splicing and Ubiquitin Modification
… found to have increased expression in breast and colon carcinoma cell lines, potentially resulting in reduction of RAD51D activity and contributing to genomic instability during carcinogenesis. These data imply that the RNF138 E3 ligase regulates RAD51D activity and may be a good therapeutic …
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Influence of Human Papillomavirus 16 E6 on TRAIL-Mediated Apoptosis
… pathways in two cellular models: HCT116 colon carcinoma cells and U2OS osteosarcoma cells. We show that transfection of the HPV 16 E6 oncogene into HCT116 cells provides protection from TRAIL-mediated apoptosis. Additionally, we demonstrate that the protection provided by E6 is …
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A New Immunoassay for Quantification of a Novel Cancer Antigen in Serum and Immunostaining of Carcinoma Tissues and Cultured Cells Revealing the Antigenic Cellular Location.
… 2) to determine the location of the antigen in carcinoma and normal cells. Sera from healthy volunteers (n = 147) and cancer patients (n = 26) were compared for both bound and total antigen concentrations using the new ELISA. Healthy volunteers were subdivided into three groups: those with a …
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The Effects of a Purified B-Group Soyasaponin Fraction on the Proliferation, Cell Cycle Progression and Induction of Macroautophagy in Hct-15 Human Colon Adenocarcinoma Cells
Colon carcinoma is the second largest cause of cancer-related mortality in the United States. Diet has been recognized as an important modifier of colon cancer development for decades. Specifically, dietary patterns that include legumes, such as soybeans, may reduce colon cancer progression. High …
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Regulatory Balance Between the Peptide Trasporter, Pept1, and Amino Acid Transporter Gene Expression in the Enterocyte
… profile of these cells. Thus, the human colon carcinoma (Caco-2) cell line was used as a small intestinal in vitro model for this study. These cells express PepT1, HPT1, PTR3 EAAT1, EAAT3, rBAT, Bo,+AT CAT1, LAT1, y+LAT1, y+LAT2, ABCC3, ABCC4, which increased from D0 to D21 post …
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Evaluating the Expression of Angiogenic Mediators in a Mouse Model of Tumor Metastasis
… cells. CT26.CL25 cells are VEGF-producing murine colon carcinoma cells transfected with the lacZ gene, which expresses B-galactosidase activity. These cells, injected intravenously, form tumor nodules in the lung. A pilot study revealed development of lung nodules in mice nine days after …
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