Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 61 for “"Collagen type I"”.
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Structural Analysis of Reconstituted Collagen Type I - Heparin Cofibrils
… comprised of 2 native ECM components like collagen type I and the GAG heparin, is presented here. Although the individual components comply with the adhesive, mechanical and bioinductive requirements for artificial reconstituted ECMs, their interaction and structural characterization …
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Multifunctional Nanocoatings Based On Biomimetic Collagen Type I For Bone Tissue Engineering Applications
Collagen heparin, layer-by-layer, scaffold, bone
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Bacteriodes fragilis interactions with the collagen type I component of the host extracellular matrix
… components of the extracellular matrix, such as collagen, is critical in bacterial colonisation and host cell invasion. The aim of this dissertation was to specifically identify the collagen type I adhesins and proteases in the common research strain, B. fragilis 638R, as well as in 23 clinical …
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Molecular Dynamics and Mechanical Behavior of Collagen Type I and its Lysine/Hydroxylysine-derived Crosslinks
Collagen type I is an extracellular matrix (ECM) protein that affords tensile strength and biological scaffolding to numerous vertebrate and invertebrate tissues. This strength has been attributed to the triple-helical structure of the collagen type I molecules, their organization into fibrils, and …
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Microfluidic Approaches to Thrombosis and Hemostasis: Towards a Patient-Specific Test of Antiplatelet Therapeutics and the Assessment of Coagulopathy in Hemophilic and Trauma Patients
… therapies on platelet aggregates adhering to collagen type I surfaces. We phenotyped individual healthy donor platelet function responses to in vivo or in vitro aspirin, a common antiplatelet therapy over collagen type I surfaces at venous shear rates. Utilizing the same flow assay, we also …
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Molecular and microscopical analysis of pathogenic streptococci - studies on surface proteins interacting with human cells and extracellular matrix
… surviving intracellularly in several human cell types. In this thesis I have investigated the interaction of S. pyogenes and Streptococcus pneumoniae (S. pneumoniae) with collagen type VI, the binding of S. pyogenes M1 protein and leucine rich (Slr) protein to collagen type I, and Slr’s …
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The regulation of matrix metalloproteinase (MMP) secretion in human vascular cells exposed to extracellular and wound matrices
… cells and macrophages - to well defined ECM (collagen type I, III and IV and laminin) and wound healing matrix proteins (fibrin and plasma fibronectin) has not yet beeen carried out.
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In vitro comparison of equine tendon- and bone marrow-derived cells expanded with FGF-2 prior to culturing with tendon matrix and IGF-I
… tendon cultures, cell viability, expression of collagen types I and II, and cartilage oligomeric matrix protein (COMP) mRNAs, collagen and glycosaminoglycans (GAG) syntheses were assessed. Tendon-derived cells proliferated significantly more rapidly in the initial monolayer expansion cultures in …
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The effect of synthetic cannabinoids on wound healing of chondrocytes monolayers and pseudo 3D cartilage tissue. Effect of different concentrations of synthetic cannabinoids WIN55, 212-2, URB602 and HU-308 with and without their antagonists on wound healing of chondrocyte monolayers and pseudo 3D cartilage tissue.
… chondrocyte monolayers. Moreover, expression of collagen type-I, collagen type-II, fibronectin and S100 proteins were detected using immunofluorescence and verified quantitatively using ELISA based techniques, following treatment with 1μM and 2μM of WIN-2, for both 2D monolayers and 3D sheets. …
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Effect of Electrospun Mesh Diameter, Mesh Alignment, and Mechanical Stretch on Bone Marrow Stromal Cells for Ligament Tissue Engineering
… Finally, expression of the ligament-like markers collagen type I and decorin were enhanced on the smallest fiber diameters compared to larger diameters. In the second study BMSCs — seeded onto aligned electrospun PEUUR scaffolds — were cyclically stretched to determine the effect of dynamic …
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Adsorptive Immobilisierung von Kollagen Typ I an Titanoxidoberflächen
… the modification with biological components. Collagen is the main component of bone and is therefore an interesting substance for the base modification of titanium implants. The aim of the work was to investigate the adsorptive immobilisation of collagen type I on titanium surfaces. The …
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Comparison of bone marrow mesenchymal stem cells and tendon progenitor cells cultured on collagen surfaces
… differentiation and gene expression of many cell types are altered by adhesion to and interaction with matrix proteins and the extracellular environment. Tendon progenitor cells reside within a niche that comprises primarily parallel collagen fibers, and this niche plays an important role in …
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The role of the mechanical microenvironment in regulation of cholangiocyte specification and tubulogenesis
… the ECM, I observed that high densities of collagen type I serve as an essential signal for the developing bile ducts, overriding the influence of substrate stiffness. This was supported by in vivo evidence, as, although collagen I is absent from the main parenchyma of the human foetal …
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Genipin-crosslinked electrospun collagen nanofibers
… and pore interconnectivity. In this research collagen type I nanofibers were fabricated using electrospinning. Collagen nanofibers were unstable in aqueous environments, and thus crosslinking was required. Glutaraldehyde, which is cytotoxic to cells, is currently the chemical crosslinking …
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The low-level light therapy affects human dermal fibroblast via TGF-β/smad signaling pathway: in vitro study
… signaling molecules 12 D. Protein expression of collagen type I, TGF-ß1, smad3 and smad7 after incubation with exogenous TGF-ß1 13 IV. DISCUSSION 17 V. CONCLUSION 22 REFERENCES 23 국문요약 28
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In Vivo Studies of the Foreign Body Reaction to Biomedical Polymers
… phosphate) coupled with collagen type I siRNA were subcutaneously implanted. A significant decrease in fibrous capsule thickness at weeks 2 and 4 were shown compared to the control nanofibers.
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Cell engineering of human bone monolayers and the effect of growth factors and microcontact printed ECM proteins on wound healing. The role of ECM proteins, TGF¿-1, 2 and 3 and HCl/BSA in cellular adhesion, wound healing and imaging of the cell surface interface with the widefield surface plasmon microscope.
… aimed at determining the role of the TGF-¿s, Collagen type I, Fibronectin and Laminin in bone cell behaviour. To do this MG63 bone cells were used to examine cell adhesion and alignment to different micro-contact printed ECM protein patterns of different widths. The study also aimed at …
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The Extraction of Type II Collagen and the Electrospinning of Nano-Fibrous Scaffolds
… functions to reduce friction and absorb shock. Collagen type II is the largest constituent in the extracellular matrix of articular cartilage and its restoration is of the highest interest to tissue engineers. Cartilage has little ability to naturally regenerate due to the absence of vascularity …
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Functional Consequences of Matrix Metalloproteinase-1 Over-Expression in Human Gliomas
… are thought to contribute to the invaseive phenotype of human gliomas. Absent in normal brain, matrix metalloproteinase-1 (MMP-1) has been shown to be present in gliomas, and in particular in glioblastoma multiforme (GBM). To begin to examine the role of MMP-1 in these tumore, two human glioma …
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Extrusion and evaluation of degradation rate and porosity of small diameter collagen tubes
… based on natural materials. The small diameter collagen tubes were extruded using bovine collagen type I. The biodegradation rate was determined and compared for small diameter collagen tubes crosslinked with glutaraldehyde and N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) …
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