Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 37 for “"Collagen IV"”.
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Integrin Distribution in Collagen Iv Mutants and Use of Rna Interference to Identify a Collagen Iv Receptor in C. Elegans
… was found to be significantly decreased in collagen IV mutants. To better understand the role of collagen IV in perlecan-integrin dependent sarcomere organization, an extrachromosomal array containing a functional ßpat-3::GFP fusion gene was mated using genetic crosses into a collagen IV …
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The use of collagen IV immunohistochemistry in the diagnosis of bullous pemphigoid
… equipment and is technically sophisticated. Collagen IV immunohistochemistry is reported as a reliable test for the diagnosis of epidermolysis bullosa acquisita whereby It stains the roof of a subepidermal blister and would be expected on the floor in bullous pemphigoid. This technique could …
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Notch Regulates Vascular Collagen IV Basement Membrane Through Modulation of Lysyl Hydroxylase 3 Trafficking
<p>Collagen type IV (Col IV) is a basement membrane protein associated with early blood vessel morphogenesis and is essential for blood vessel stability. Defects in vascular Col IV deposition are the basis of heritable disorders, such as small vessel disease, marked by cerebral hemorrhage and …
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Establishment of a Novel Patient hiPSC-derived in vitro Blood-Brain Barrier Model of Collagen IV Small Vessel Disease
… affecting the elderly. However, mutations in Collagen IV α1/α2 (COL4A1/2), a highly abundant matrisome protein, cause monogenic SVD, which shares many clinical features with the sporadic form. In order to better understand the pathomolecular mechanisms leading to SVD, human induced pluripotent …
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Modelling the gastric epithelium for testing of new chemical entities
… Coating the polycarbonate filter with collagen IV, rather with collagen I, enhanced tight junction formation. TEER for cells grown on Transwells coated with collagen IV was close to that obtained with intact guinea-pig gastric epithelium in vitro. Differentiation was assessed by …
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Spatiotemporal controlled delivery of nanoparticles to injured vasculature
… multimodal nanoparticles (NP) that target and deliver therapeutic agents to a site of disease are a promising direction in modem medicine. As a starting point for innovation, we designed a hybrid NP system combining the benefits of liposomes and polymeric NPs. These particles have a polymeric …
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Nutritional regulation of neural stem cell reactivation in Drosophila melanogaster
… neural stem cell (NSC) quiescence and reactivation is a key point of regulation during neurogenesis, and in Drosophila, this process is tightly coupled to nutrient availability. Post-embryonic NSCs in Drosophila only exit quiescence when larvae are fed a diet containing essential amino …
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Acidic Pericellular Ph: Effects On Proteolysis And Gene Expression As Determined In 3d Models Of Breast Carcinoma
… is an acidic peritumoral pH. The first objective was to determine if an acidic extracellular pH observed in vivo (i.e., pHe 6.8) affects the activity of proteases, such as cathepsin B, that contribute to degradation of collagen IV by tumor cells when grown in biologically relevant …
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Modulation of TGF-beta and extracellular matrix in retionic acid-induced cleft palate
… matrix. The patterns of localization of collagen I, III, and IV, fibronectin, tenascin, laminin and hyaluronate were investigated in normal palate development. Collagen I and collagen IV, laminin, and hyaluronate proved to have spatial-temporal patterns of localization during palatal …
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Verstärkung der Zelladhärenz und Induktion des Zell-Spreading - eine neue Funktion von RAGE, einem hoch selektiven Differenzierungsmarker humaner Alveolar-Typ 1-Zellen
… involves a number of structurally diverse molecules, which derive from multiple complex rearrangements of reducing sugars with free amino-groups of proteins. They evolve during food production and also in vivo during ageing and to an accelerated degree in diabetes, where AGEs cause …
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In Vitro Binding and Transport Regulation by Endothelial Cells: Preliminary Studies looking at FIX and IGF-I
… form an important barrier for vascular drug delivery. This thesis contains preliminary studies targeted at understanding the mechanisms of binding and transport across endothelial cells cultured in vitro. Specifically, the first study investigates how the recombinant source of Factor IX (FIX), a …
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Neuroglial differentiation and neo-innervation in extracellular matrix-based tissue engineered innervated smooth muscle sheets
… by an extracellular matrix composed primarily of collagen IV, laminin and heparan sulfate. Disruption of ganglionic integrity, loss or damage of all or specific subsets of enteric neurons occur in several pathological states resulting in intestinal dysmotility and reduced quality of life. …
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ADAM10: a Novel Regulator of Mast Cell Function and Activation
… expressed on mast cells both in vitro and in vivo. Its expression is inhibited by IL-10, a suppressive cytokine. siRNA depletion of ADAM10 on bone marrow-derived mast cells (BMMC) caused decreased IL-6 production following IgE cross-linking and also impaired BMMC stem cell factor (SCF)-induced …
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Engineered microenvironments for studying liver progenitor differentiation
The bipotential differentiation of liver progenitor cells underlies liver development and bile duct formation as well as liver regeneration and disease. Both TGFβ and Notch signaling are known to play important roles in the liver progenitor specification process and subsequent tissue morphogenesis. …
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In Vitro Remodeling of Extracellular Matrix Following Mild Traumatic Brain Injury
Every year millions of individuals suffer from traumatic brain injury (TBI) leading to permanent disabilities and even death. Mild TBI (mTBI) is the most common form of TBI comprising about 80-90% of all occurrences. Following a CNS insult like an mTBI, astrocytes can undergo activation resulting …
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Expression and role of the extracellular matrix protein tenascin in ovarian cancer
… isoforms, which are produced by alternative splicing of the RNA transcript. The pattern of different isoforms expressed was investigated using RT-PCR and hybridisation to specific oligonucleotides. The smallest TN splice variant was found in all tumours examined while the appearance of …
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Development of a physiologically accurate 3D blood-brain barrier hydrogel model
… degraded and can lead to further neurodegenerative disorders such as Alzheimer's and dementia. In order to better understand the structure of the BBB, the causes of its degradation, and potentially curing diseases, drug delivery experiments have been performed on the brain. However, since the …
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Functional Characterization of a Cathepsin L in Drosophila Melanogaster
… Primordium (ASP) is a tracheal tube that invasively grows toward and into the wing imaginal disc. The unfolding of Drosophila wing is a process following eclosion with a cuticular bilayer replacing epithelial cells originally packing the wing. We reasoned that protease functions might be needed …
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Beta 3 integrins : negative regulators of angiogenesis
… tumstatin, a fragment of the alpha 3 chain of collagen IV, inhibits proliferation, inhibits total protein synthesis and specifically inhibits CAP-dependent protein synthesis in MLEC. These effects do not occur when beta 3-null MLEC are treated with tumstatin or any of its derivatives. Nor do …
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