Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 69 for “"Collagen I"”.
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Breast Cancer Cell Mechanical Properties and Migration on Collagen I Matrices with Tunable Elasticity
Unlike their normal counterparts, breast cancer cells break free from epithelia and invade the underlying stroma, leading to deadly metastases. Cell migration requires mechanical interactions between the intracellular cytoskeleton and the extracellular matrix. The goals of this work were to compare …
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Regulation of Bovine Mammary Epithelial Cell Response by Autocrine IGF-I and by Collagen I
… cells and is crucial for mammary development. Collagen I is an extracellular matrix protein (ECM) found in skin and in other connective tissues throughout the body. The guiding question in this dissertation was how IGF-I signaling and how binding protein profile were influenced by autocrine …
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Understanding collagen-l folding and misfolding
Chapter One: Introduction to Type I Collagen and Osteogenesis Imperfecta Collagen-I is the primary proteinaceous component of skin, bone, and tendon. Disruptions in collagen-I homeostasis, typically due to non-synonymous mutations in collagen-- encoding genes, cause a variety of severe incurable …
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Type I collagen proteostasis
The folding, quality control, and secretion of collagen presents a significant challenge to collagen-producing cells. Each monomeric polypeptide must undergo extensive post-translational modifications, folding and assembly of the C-terminal propeptide globular domain, and isomerization of hundreds …
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Distant chaperones and N-glycan signals : new mechanisms of secretory pathway proteostasis
… to accommodate challenging cargoes such as collagen-1. In the human body, collagen-I constitutes the primary protein component of bone, skin, and other organs; collagen-I misfolding results in pathological ER stress and connective tissue diseases. We therefore set out 1) to identify cellular …
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Modulation of TGF-beta and extracellular matrix in retionic acid-induced cleft palate
… matrix. The patterns of localization of collagen I, III, and IV, fibronectin, tenascin, laminin and hyaluronate were investigated in normal palate development. Collagen I and collagen IV, laminin, and hyaluronate proved to have spatial-temporal patterns of localization during palatal …
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A three-dimensional in vitro tumor model representative of the in vivo tumor microenvironment
… cellulose, electrospun polycaprolactone/collagen I, and highly porous electrospun poly(L-lactic acid). However, cancer cells cultured on these scaffolds demonstrated poor adhesion, sufficient adhesion with poor infiltration, and increased but still inadequate infiltration, respectively. …
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The C-Propeptide in Collagen Proteostasis
Collagen folding is initiated at the C-terminal propeptide (C-Pro) domain, a globular domain wholly distinct from the collagen’s characteristic triple helix. The C-Pro domain is responsible for assembling three collagen strands into the correct orientation and stoichiometry and nucleating folding …
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Modelling the gastric epithelium for testing of new chemical entities
… Coating the polycarbonate filter with collagen IV, rather with collagen I, enhanced tight junction formation. TEER for cells grown on Transwells coated with collagen IV was close to that obtained with intact guinea-pig gastric epithelium in vitro. Differentiation was assessed by …
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Identifying the Molecular Cause of Extreme Endoplasmic Reticulum Dilation in Pediatric Osteosarcoma and Its Relationship to the Disease
… that has resulted in the accumulation of procollagen-I protein within ‘hallmark’ dilated ER. Using CRISPR activation technology, increased expression of only SAR1A and SEC24D to physiologically normal levels was sufficient to restore both collagen-I secretion and resolve dilated ER morphology …
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Characterization of the cardiogenesis of embryonic stem cells
… systematically studied. Next, the effects of collagen I and cell alignment, two biophysical signatures of the adult myocardium, on promoting phenotypic changes of isolated embryonic stem cell derived cardiomyocytes (ESCDMs) were investigated. Effects of collagen I depended on how it was …
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Investigating the Molecular Mechanisms of SNED1 Fiber Assembly in the Extracellular Matrix
… presence of two ECM proteins, fibronectin and collagen I, for its fibrillar assembly. In addition, using biolayer interferometry, we demonstrated that SNED1 could directly bind to collagen I, which is the first experimentally validated binding partner of SNED1. I also examined the role of …
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A COMPARATIVE STUDY OF A PATHOGENIC VERSUS A NONPATHOGENIC NAEGLERIA SPECIES
… components (ECM), laminin-1, fibronectin, and collagen I, was assessed. N. fowleri exhibited a higher level of adhesion to ECM components and was shown to invade tri-dimensional ECM scaffolds (matrigel and collagen I) to a greater extent than N. lovaniensis. Scanning electron microscopy …
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von Willebrand factor interaction with subendothelial collagens and platelet surface receptor GPIBalpha under shear conditions
… and thrombosis. By binding both subendothelial collagens and platelet surface receptors GPIbalpha, multimeric vWf acts as a bridge between subendothelium and circulating platelets. In this study, the molecular binding between vWf multimers and human pepsin-digested collagen I, III and VI was …
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Collagen structure within aneurysm tissue in bicuspid aortic valve patients
… of research points to changes in the elastin and collagen composition of the aneurysm tissue as a key component influencing the course of the disease. The purpose of this thesis is to clarify collagen's contributions to aneurysm development, progression, and clinical consequences by examining its …
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The role of the mechanical microenvironment in regulation of cholangiocyte specification and tubulogenesis
… the ECM, I observed that high densities of collagen type I serve as an essential signal for the developing bile ducts, overriding the influence of substrate stiffness. This was supported by in vivo evidence, as, although collagen I is absent from the main parenchyma of the human foetal …
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IN VITRO MODULATION OF HUMAN CORNEAL ENDOTHELIAL CELL BEHAVIOR VIA TOPOGRAPHICAL AND BIOCHEMICAL CUES
… are extracellular matrix coating of fibronectin-collagen I, FNC Coating Mix, and laminin-chondroitin sulfate. Our results indicate that topographical and biochemical cues are able to alter HCEC behavior. Engineered environment may thus enable cellular behavior fine-tuning.
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Molecular and microscopical analysis of pathogenic streptococci - studies on surface proteins interacting with human cells and extracellular matrix
… Streptococcus pneumoniae (S. pneumoniae) with collagen type VI, the binding of S. pyogenes M1 protein and leucine rich (Slr) protein to collagen type I, and Slr’s interaction with human keratinocytes. The adherence of S. pyogenes and S. pneumoniae to collagen type VI in murine upper and lower …
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Development and Characterisation of a Vessel-On-a-Chip Model of Inflammation and Inflammatory Haemostasis
… cultured against a mixed matrix of geltrex and collagen I, compared to collagen I alone. Response to pharmacological inhibitors was also influenced by ECM composition. The potential use of the model in drug discovery was investigated during a secondment at the funding body, AstraZeneca. Several …
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EFFECT OF COMBINATION OF THE MAMMALIAN LIGNAN, ENTEROLACTONE, WITH TYROSINE KINASE INHIBITORS ON MARKERS OF HEPATIC FIBROSIS
… changes of several fibrotic markers (e.g., collagen I, -SMA, MMP2/MMP9, and TIMP-1,) using human hepatic stellate cells (HSC), LX-2 cells, at both mRNA and protein level by real-time PCR and ELISA/western blot, respectively, demonstrated that Gefitinib had the greatest ability to attenuate …
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