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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 133 for “"Coiled coil"”.
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Novel plant nuclear envelope-associated coiled-coil proteins
… NEAP protein structure consists of extensive coiled-coil (CC) domains, followed by a nuclear localisation signal (NLS) and a C-terminal transmembrane (TM) domain. Confocal microscopy shows that fluorescent protein tagged NEAP proteins localise to the nuclear periphery as part of highly …
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Statistical prediction schemes for the coiled-coil motif
… and distinguishing trimeric and dimeric coiled coils. Practical issues in the implementation of the two-stranded coiled coil prediction algorithm PairCoil suggested by Berger are discussed. This algorithm is naturally extended to the domain of three-stranded coiled coils in the …
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Computational prediction of coiled-coil interaction structure specificity
The alpha-helical coiled coil is a protein sequence and structural motif that consists of two or more helices in a parallel or antiparallel orientation supercoiling around a central axis. Coiled coils have been observed in a wide range of protein families, and many studies have focused on their …
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Multicoil-HMM : improved prediction of coiled-coil oligomer state from sequence
The Multicoil-HMM algorithm offers improved prediction of coiled-coil oligomerization state. The algorithm combines the pairwise correlations of the Multicoil method with the flexibility of HMM methods. The resulting method incorporates predictors deemed important by a multinomial logistic …
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Investigation of coiled-coil interactions between proteins of the spindle pole body
… proteins are predicted to contain one or more coiled coils. The coiled coil is a common protein-protein interaction domain, consisting of two or more supercoiled [alpha]-helices. The high frequency of coiled coils predicted in SPB proteins suggests that this structure may be important for …
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Programmable nanomaterials via hierarchical assembly of computationally designed parallel folding coiled coil peptide bundlemers
… materials through computationally engineered coiled coil bundlemers, which function as supermolecular building blocks for both physical self-assembly and covalent network formation. By integrating molecular modeling, experimental synthesis, and advanced structural characterization, this work …
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Anatomy of the coiled coil and its role in the conformational change of influenza hemagglutinin
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1995.
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The Rational Design of Coiled-Coil Peptides towards Understanding Protein-Crystal Interactions and Amorphous-to-Crystalline Transitions
… research and rational peptide design using the coiled-coil peptide motif and calcium hydrogen phosphate dihydrate (CaHPO4 x 2H2O), commonly known as brushite. Coiled-coils were designed to alter the complete growth pathway of brushite beginning with an amorphous precursor and resulting in a …
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The role of the rho-associated coiled-coil containing kinase (ROCK) in cytokine-induced chemokine responses in intestinal epithelial cells
<p>Inflammatory Bowel Disease (IBD) affects over 1 million Americans and can cause severe tissue damage and even death. TNF-α is a pro-inflammatory cytokine that is elevated in IBD and plays a central role in inflammation. Previous results in our laboratory showed that another cytokine, IL-1β, …
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Structural specificity in coiled coils : a and d position polar residues
… at the a and the d positions of a reference coiled coil, GCN4-pVL. The reference coiled coil is very stable in solution and exists as a mixture of dimers and trimers. The placement of single polar residues in the otherwise hydrophobic core of GCN4-pVL has dramatic effects on both stability …
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The Middle Region is Required for Phosphorylation Regulation of Ceramide Transfer Protein
… Middle Region (MR) which contains a predicted coiled-coil region. This coiled-coil region is hypothesized to mediate oligomerization of CERT, yet the role of MR is unknown. In this study, we primarily used biochemical assays to investigate the role of MR in CERT function. Our data shows MR is …
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Structure and Dynamics of AcrA, a Periplasmic Component of a Multidrug Efflux Pump
… AcrA contains an interrupted alpha-helical, coiled-coil domain flanked by a pair of beta-stranded lipoyl motifs, and my SDSL/EPR analysis revealed that the pH-induced conformation change mainly involves the coiled-coil and the lipoyl domains. In addition, I found that each AcrA monomer folds …
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SERS-active peptide nanoparticles for investigating protein allostery and bionanoassembly
… the development of biocompatible nanomaterials. Coiled coil peptides have demonstrate highly specific binding activity in a variety of cellular functions and their structure is well understood. Work presented in chapter 4 of this thesis documents pH sensitive nanoparticle assembly driven by …
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Structural and Molecular Mechanisms of Centrosome Assembly and Strength
… by interactions between multiple dispersed coiled-coil domains. Structural analyses of a phosphorylated region (PReM) in SPD-5 revealed a helical hairpin that dimerizes to form a tetrameric coiled-coil. Mutations within this structure and other interacting regions cause PCM assembly defects …
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Network tools forthe analysis and prediction of protein-protein interactions
… Our second computational platform, the Coiled-Coil Database (CCDB), serves as a central and easily queryable repository for information about the coiled coil protein structural motif in a variety of organisms.
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Investigation of the relative conformational stability of protein mutants by molecular dynamics simulation
… as on their lowest aggregation level, namely the coiled coil structure. Mutations in the helix termination motif of human hair keratin gene hHb6 seem to be connected to the hereditary hair dystrophy Monilethrix. The most common mutations reported are Glu413Lys and Glu413Asp, located at the …
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Characterization of Novel Neuronal Antigens in the Central Nervous System of Manduca Sexta
… to several members of a growing family of coiled-coil, Golgi-associated proteins, including GM-130 and golgin-95. The Drosophila and Caenorhabditis elegans homologs of Ms-golgin80 were also identified through homology sequence search. These proteins share a characteristic feature of having …
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The essentiality of DivIVAEf oligomerization for proper cell division in enterococcus faecalis and interaction with a novel cell division protein
… homologues is the ability to oligomerize through coiled-coil interaction into complexes comprising 10-12 monomers. To date, the importance of DivIVA oligomerization and the N-terminal coiled-coil for its proper function in bacterial cell division has not been reported. This study examined the …
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Modular design of coiled coils to target bZIP transcription factors
… tight and specific binders of the leucine-zipper coiled-coil domain. Both methods tested take advantage of the structure and biophysical properties of a coiled coil. In the coiled-coil dimer, two helices wrap around each other into a super helix. At the sequence level, coiled coils have a …
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