Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"Coil interactions"”.
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Investigation of coiled-coil interactions between proteins of the spindle pole body
… most cases, it is not known precisely how these interactions occur. Interestingly, a large proportion of SPB proteins are predicted to contain one or more coiled coils. The coiled coil is a common protein-protein interaction domain, consisting of two or more supercoiled [alpha]-helices. The high …
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Structural and Molecular Mechanisms of Centrosome Assembly and Strength
… for mitotic spindle assembly. The molecular interactions that enable PCM to assemble and resist external forces are poorly understood. To answer such question, we use cross-linking mass spectrometry (XL-MS) to analyze PLK-1-potentiated multimerization of SPD-5, the main PCM scaffold protein …
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Mechanism of Host Recognition by the Bacteriophage <i>Cystoviridae</i>
… We hypothesize that fusion occurs through coil-to-coil interactions. To observe details of the lifecycle of Phi 8 we utilized in situ cryo electron microscopy. Our results indicate the presence of <em>Cystoviridae</em> in different stages of capsid biogenesis. Lastly, we expressed different …
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Engineering protein self-assembly for biosensing applications
… assembled through controlled protein-protein interactions and genetically encoded biosensing functionalities tailored for biomedical and environmental applications. First, we demonstrate a protein coating self-assembly strategy that modularly incorporates functional protein domains. This …
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Biophysical and structural characterization of components from the nuclear pore complex and the ubiquitin pathway
… composed of three proteins and stabilized by coiled-coil interactions. Here we reconstitute a tetrameric assembly between the Nspl-complex and a fourth nucleoporin (Nup) Nic96. Nic96 harbors a 20 kDa coiled-coil domain at the N-terminus followed by a 65 kDa stacked helical domain. The …
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Explorations into protein structure with the knob-socket model
… is that they have a preferred handedness, some interactions are ancillary to the packing interaction, there are specific amino preferences for specific positions in packing surfaces, and there is no relationship between side-chain rotamer of the knob packing into the tertiary packing surface. …