Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 58 for “"Coculture"”.
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Mobilisation, Isolation and Coculture of Haematopoietic Stem Cells
… the communication between HSCs and the niche, coculture assays with mesenchymal stromal cells (MSCs) were performed in vitro. Here, we can demonstrate that cell-to-cell contact has a significant impact on hematopoietic stem cells expansion, migratory potential and stemness. In this study (part …
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Exploring genomic complementarity in a naturally occurring coculture: A case study of Methylocystis echinoides rim and Hyphomicrobium sulfonivorans
Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2025-10-19 without embargo terms
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Identification of mechanisms of gemcitabine resistance using a whole genome shRNA screen in a novel in vitro coculture model of pancreatic cancer.
… validating, and evaluating mechanistically a coculture model of gemcitabine resistance, established previously. I demonstrated that the gemcitabine resistance observed in coculture was a product of cell-cell contact, transient in nature, and related to cell density-mediated signalling …
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Increased differentiation properties in two- and three-dimensional coculture of hepatocytes and liver epithelial cells by a novel quantitative functional liver assay
… rat liver epithelial cells (LEC), lig8, grown in coculture with mature liver cells in a three dimensional, perfused microreactor optimized for hepatocyte culture. Lig8 was derived by suppression of the asymmetric growth kinetics that may be indicative of stem cells, and Lig8 progeny can be induced …
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A Tissue-Engineered Microvascular System to Evaluate Vascular Progenitor Cells for Angiogenic Therapies
… we investigated the following hypotheses: (1) cocultures of human umbilical cord blood-derived endothelial progenitor cells (hCB-EPCs) with mural cells can produce the microenvironmental cues necessary to support physiological microvessel formation in vitro; (2) poly(ethylene glycol) (PEG) …
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Influence of Fibroblasts on Metastatic Cancer Cell Drug Resistance in a 3D Microfluidic Cell Array
… breast cancer cell proliferation in the 2D coculture, but the cancer cell growth rate increases in the 3D static and device coculture. Additionally, preliminary quantitative image analysis showed that the coculture condition had lower apoptosis indicated by caspase-3 activity after …
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Ethanolamine as a cardioprotective agent in doxorubicin-induced cardiotoxicity
… isolated murine cardiomyocytes and H9c2/3T3 coculture. Metabolic activity and cell viability were determined using MTT and LDH assays for H9c2, fibroblasts and coculture experiments, and trypan blue exclusion and LDH assays in murine cardiomyocytes. Apoptotic activity was measured in …
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Protective effects of Human Bone Marrow-Derived Mesenchymal Stem Cells on Apoptosis Induced Human Neuroblastoma SH-SY5Y Cells
… SH-SY5Ys. hMSCs plated in transwell were cocultured with STS-treated SH-SY5Ys. Cell viability was assessed by trypan blue dye exclusion method and MTS assay for 5days. Morphological, biochemical and Bcl-2 family western blot analysis were also performed. Number of viable cells was …
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Quasivirus Infection Model Identifies a Novel Role for Protein Arginine Methyltransferase 1 in the HPV Lifecycle
… this work I show the combination of 2D monolayer coculture (coculture) and HPV quasivirus infection are an optimal system for investigating HPV host pathogen interactions at early timepoints post infection. HPV viral entry requires heparin sulfate moieties, extracellular proteases, and actively …
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Applications for human neural stem cell-derived motor neurons in amyotrophic lateral sclerosis: Cell replacement therapy and disease modeling
… at the disease end-stage. Using an in vitro coculture system, we provided evidence that human mutant SOD1(G93A)-expressing primary microglia, isolated after disease onset, were directly toxic to hNSC-derived motor neurons. Additionally, normal astrocytes not only lost their protective …
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Population oscillations, synchronization, and range expansion in a bacterial cross-protection mutualism
… (ampicillin and chloramphenicol) so that the coculture can survive in antibiotic concentrations that inhibit growth of either strain alone. Moreover, we find that daily dilutions of the coculture lead to large oscillations in the relative abundance of the two strains, with the ratio of …
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Characterization of an Alternatively Spliced Platelet-Derived Growth Factor Variant During Myelin Synthesis
… dorsal root ganglion (DRG) neuron/Schwann cell cocultures and shown to regulate myelin synthesis. mRNA transcripts of the long-form PDGF-A chain variant (LF-PDGF-A) were detected by RT-PCR in cocultures. Its expression level increased during active myelin synthesis in contrast to those of the …
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Bacterial interactions and enzyme kinetics in environmental polyester degradation
… toward polymer degradation assessed via coculture respiration assays, monoculture growth measurements, DOC accumulation and removal tracking, and quantification of specific polymer degradation products. The results show depolymerization was controlled by a bacterium that metabolized only …
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CROSSTALK BETWEEN GLIOMA CELLS AND TUMOR ASSOCIATED-MACROPHAGES: MECHANISMS AND THERAPEUTIC STRATEGIES
… or mutant IDH genes, through direct and indirect cocultures. Then we investigated the mechanisms underlying the macrophages-glioma cells’ crosstalk in hypoxia, a key aspect of the tumor microenvironment. In addition, in the context of nanovectors’ strategy, we set coculture models of macrophages …
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Engineering hydrogel environments to direct cell fate
… cellular response, and created PEG based 3D coculture environments to study the interface of cell types for increased angiogenesis.
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Transcriptional Analysis of Reciprocal Tumor-Microenvironment Interactions in Glioblastoma
… expression data from mixed physical coculture of GBM cells and ECs, we identified a previously undescribed upregulation of the cAMP specific phosphodiesterase PDE7B in GBM cells in response to ECs. We further found that elevated PDE7B expression occurs in most GBM cases and has a …
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Investigation of Specialized Metabolites in Apicomplexan Parasite Life Cycles
… Through structure-, bioinformatic-, and coculture-guided approaches, we have uncovered chemical space within the mosquito-microbiome and evaluated how microbial-produced small molecules influence the parasite during its vector stage. We have also expanded our knowledge of parasite-produce …
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Enhancing energy recovery from industrial wastewater using microbial fuel cells
… Initial studies investigated the use of defined cocultures as a way of improving turnover of substrate and hence electricity produced by exploiting mutualistic relationships such as syntrophy or ability of facultative microoganisms (Saccharomyces cerevisiae) to consume residual oxygen from the …
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Identification of a gap junction communication pathway critical in innate immunity
… the use GFP reporters, in vitro transplant coculture systems, and in vivo models of infection and sterile injury, this thesis describes identification of a gap junction intercellular communication pathway for amplifying immune and inflammatory responses, and demonstrates its importance in …
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The characterisation of dendritic cell, microglial, macrophage and T cell responses during mycobacterial infection of the central nervous system
… DCs from CLNs of CNS-TB mice were cocultured with naïve CD3+ T cells to generate a DC-T cell coculture, cells were sorted using fluorescence-activated cell sorting (FACS). DC-T cell coculture demonstrated increased percentage expression of IFNg, IL-4, IL-10 and TGF-b responses by …
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