Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"Co-immunoprecipitation (Co-IP)"”.
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Elucidating the Function of Krüppel Homolog 1 (Kr-h1) Associated Proteins (KAPs) in Aedes aegypti Reproduction Through RNA Interference-Mediated Downregulation
The transcription factor Krüppel homolog 1 (Kr-h1) is crucial in multiple reproductive processes of Aedes aegypti mosquitoes, including previtellogenesis, vitellogenesis, and oogenesis. This study explores the interaction between Kr-h1 and its potential associated proteins (KAPs), with a specific …
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Genetic Approach to Discover ARMC4 as a Novel NF-κB Negative Regulator and Tumor Suppressor in Colorectal Cancer
… a powerful technique established by us, we discovered armadillo repeat containing protein 4 (ARMC4) as a novel negative regulator of NF-κB in colorectal cancer (CRC). ARMC4 is a rarely studied protein only known to date for its role in primary ciliary dyskinesia (PCD) and mouse spermatogenesis. …
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Co-regulation of the Smooth Muscle Cell Alternative Splicing Programme by the RNA-Binding Proteins RBPMS, RBFOX2, MBNL1, and QKI
… of vascular smooth muscle cells (VSMCs) from a contractile /differentiated state to a synthetic/ proliferative state is a hallmark of many cardiovascular diseases, driven in part by extensive alternative splicing (AS) regulation. RBPMS, a master splicing regulator in differentiated VSMCs, is …
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The Secretory Pathway Calcium ATPase 2C promotes increased cytosolic calcium levels
… SPCA2C was expressed in HEK293A cells. Co-immunofluorescence (Co-IF) determined subcellular localization. Using Fura2 imaging, cytosolic Ca2+ concentration [Ca2+] was examined during rest, SOCE and secretagogue-stimulated signaling. Effects of SPCA2C over-expression were also examined in …
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Controlling Pseudomonas aeruginosa Persister Cells by Human Granulocyte Macrophage Colony-Stimulating Factor
… state when the antibiotic treatments are discontinued. During bacterial invasions, the host immune cells secrete special signaling proteins, known as cytokines which orchestrate events leading to human immune response and elimination of bacterial pathogens. However, compared to the well …
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Molecular mechanisms involved in the regulation of beta-cell viability by Signal Regulatory Protein-Alpha (SIRPα)
… cytokine pathways, acting via STAT1 and STAT2, contribute to β-cell death whereas anti-inflammatory mechanisms (operating via cytokines such as Interleukin-13 (IL-13)) are downregulated. IL-13 has been shown to protect β-cell viability via STAT6 signalling, and preliminary data suggested that …
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The Role of ATP and Cleavage Factor Phosphorylation in pre-mRNA 3' Cleavage Reaction
… a functional messenger RNA (mRNA) transcript. It is long-known that ATP can significantly stimulate the in vitro cleavage of adenovirus type 2 L3 (Ad2L3) RNA substrate. Here, we used ATP analogs in structure-activity assays to show that the structural features of ATP and its analogs …
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Interactions between IP3 receptor and Kras-induced actin-interacting protein
Inositol 1,4,5-trisphosphate receptors (IP3R) function as intracellular channels, facilitating the release of Ca2+ from the endoplasmic reticulum (ER) upon IP3 binding. Before responding to IP3 in intact cells, IP3R must undergo a process called 'licensing,' involving the association with …
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Regulation of Antimicrobial Peptide Genes in the Tobacco Hornworm Manduca sexta
… as natural or non-specific immune system, is conserved from insects to humans. The insect innate immune system is composed of humoral and cellular components. Insect humoral responses mainly include synthesis of antimicrobial peptides (AMPs) and activation of the prophenoloxidase (PPO) system. …
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Myxoid Liposarcoma: Models and Mechanisms of Sarcomagenesis and Response to Radiation Therapy
<p>Myxoid liposarcoma (MLPS) is a malignant soft tissue sarcoma characterized by a pathognomonic t(12;16)(q13;p11) translocation that produces a fusion oncoprotein, FUS-CHOP. This cancer is remarkably sensitive to radiotherapy and exhibits a unique pattern of extrapulmonary metastasis. However, the …
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Discovery and characterization of regulatory mechanisms affecting the heteromeric acetyl-coenzyme a carboxylase in Arabidopsis
… all organisms to generate fatty acids. A staple component of this pathway is the enzyme acetyl-CoA carboxylase (ACCase), which catalyzes the committed step by converting acetyl-CoA to malonyl-CoA. The heteromeric form of this enzyme requires four different subunits for activity: biotin …
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The Impact of Candida albicans Glycogen on Fitness, Virulence and Host Response
… fungal pathogen that presents in healthy persons colonizing the oropharyngeal, esophageal, and gastrointestinal mucosa. This polymorphic fungal pathogen remains a leading cause of both invasive and superficial mycoses. C. albicans can cause oropharyngeal candidiasis (OPC) and disseminated …