Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 159 for “"Co-immunoprecipitation"”.
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Identification of the Pokeweed Antiviral Protein Interactome by Co-Immunoprecipitation-Mass Spectrometry
… and therapeutics, as well as in agriculture, to confer disease resistance in transgenic crops. However, little is known about the biological function of RIPs in their native context. Phytolacca americana, the American pokeweed, produces a RIP called pokeweed antiviral protein (PAP). The objective …
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Analysis of the interaction between the co-chaperone p23 and the aryl hydrocarbon receptor
… domain was cloned, purified in denatured conditions from bacteria, refolded via limited dialysis, and analyzed for proper refolding via co-immunoprecipitation with the known binding partner SRC-1. This AhR NΔ515 transactivation domain construct was used, along with amino terminal AhR …
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Defining the functional role of MAGE-C1 in Multiple Myeloma
… and techniques to study MAGE proteins by comparing MAGE-C1 to better described CTAs: MAGE-C2 and MAGE-A1. Firstly, the expression of MAGE-C1, MAGE-A1, MAGE-C2 and their respective binding partners, Ski interacting protein (SKIP) and Ring-box 1 (RBX1), was confirmed in RPMI-8662. Next, …
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Characterisation of oncogenic pathways driving the pathogenesis of prostate cancer
… endopeptidase, is highly expressed in PCa and correlates with poor prognosis. LGMN is vital to PCa cell viability and migration, shown through RNAi targeting and selective drug inhibition. HSD17B4 was identified as a novel LGMN interactor through a BioID system, which employs promiscuous biotin …
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The Interaction of Early Growth Response Gene 1 and Myocyte Enhancer Factor 2C in the Murine Brain Cortex
Early growth response gene – 1 (Egr1) encodes a protein widely present in mammalian body, such as connective tissue, cardiac tissue, the liver, and the brain. As a transcription factor (TF), it is involved in processes that take place in the endocrine, digestive, immune, musculo-skeletal and …
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Association of Protein Phosphatase 2A with S6 Kinase Is Regulated In an MTOR-Dependent Manner
… protein synthesis and cell growth. Strict control of the components of this pathway is essential for cells to reach an appropriate size. Aberrant mTOR signaling has been implicated in multiple diseases such as cancer, diabetes, and heart failure. Activation of mTOR by nutrients and growth …
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The analysis of Autographa Californica multiple Nucleopolyhedrovirus EXONO (ORF141) function and its role in virus budding
… EXON0 affects BV production was investigated. Confocal microscopic analysis showed that EXON0 localized in the nucleus in the ring zone of virogenic stroma where nucleocapsids are assembled. In addition EXON0 also concentrated in the cytoplasm at the plasma membrane. Analysis of virions …
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The human Klotho VS variant: focus on the processing and function of the V, S and VS isoforms
… dramatic phenotype of KL deficient mice and its connection to signaling pathways implicated in aging. The KLVS variant consists of the F352V (KLV) and C370S (KLS) substitutions. It was detected in genome wide association studies (GWAS) that linked it to alterations in longevity and disease risk. …
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Investigation of Protein-Protein Interactions among Nicotine Biosynthetic Enzymes and Characterization of a Nicotine Transporter
Alkaloids are a class of plant secondary metabolites produced in about 20% of plant families. Domesticated tobacco, Nicotiana tabacum produces nicotine as the predominant alkaloid. The biosynthesis of nicotine occurs exclusively in the roots of tobacco, yet accumulates in the leaves of tobacco …
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Studying the Interaction of RACK-1 and GLD-1 in the Germline of Caenorhabditis elegans
… lab has been studying the influence of highly conserved scaffold protein RACK-1 (Receptor for Activated C Kinase 1) and translational repressor GLD-1 (Defective in Germline Development 1) on the proliferation differentiation decision of GSCs. RACK-1 is involved in ribosome assembly and the …
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Regulation of inflammatory TLR signalling by the BCAP adaptor
… of BCAP TIR domain interactions. The second aim is to characterise and further explore the BCAP interactome, in the context of TLR and BCR signalling. Identification of novel interaction partners and a more detailed understanding of existing interactions, including PI3K and PLC-𝛾2, is …
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Identifying Novel Interaction Between the E3 Ubiquitin Ligase Ring Finger Protein 20 (RNF20) and the Deubiquitinating Enzyme Ubiquitin Specific Protease 7 (USP7)
… epigenetic regulation, DNA damage response and oncogenic processes. In this study, RNF20, which is an E3 ligase, has been identified as a novel substrate for USP7. Through various biochemical and functional assays, including endogenous co-immunoprecipitation, comparing parental and USP7 knockout …
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Identifying Novel Interaction Between CDK8 Kinase Module and The Deubiquitinating Enzyme Ubiquitin Specific Protease 7 (USP7)
… thesis explores the role of USP7 in regulating components of gene expression regulators. The gene expression of target genes is fine-tuned by the transcriptional co-activator, the Mediator complex. Our group has determined that Cyclin Dependent Kinase 8 (CDK8) and Mediator Complex Subunit 12 …
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THE ROLE OF ADARS-INTERACTING RNA HELICASES IN SHAPING THE RNA EDITOME IN CANCER
… in cancers. However, the regulatory mechanisms controlling editing frequency in cancer cells remain largely elusive. Besides factors affecting the expression and activity of ADAR proteins, ADARs-interacting proteins are known to orchestrate editing regulation. From our co-immunoprecipitation …
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Deciphering the mechanism by which humanin binding to IGFBP3 inhibits neuronal cell death in alzheimer’s disease
… (AD). The neuroprotective peptide humanin (HN) counteracts this IGFBP3-induced cell death. Unfortunately, the natural synthesis of this peptide decreases with age coincident with the time that people are likely to develop AD. In addition, the precise mechanism of action of HN is currently …
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Nrf2-Dependent Overexpression of Sulfiredoxin and Peroxiredoxin III in Human Lung Cancer, Especially Squamous Cell Carcinoma
"TABLE OF CONTENTS ABSTRACT i TABLE OF CONTENTS iii LIST OF FIGURES v ABBREVIATION vi Ⅰ. INTRODUCTION 1 Ⅱ. MATERIALS AND METHODS 6 A. Materials 6 B. Subjects 6 C. Cell culture and viability 7 D. Transfection and depletion of siRNA 7 E. Immunocytochemistry 8 F. Co-immunoprecipitation and immunoblot …
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Elucidating the Function of Krüppel Homolog 1 (Kr-h1) Associated Proteins (KAPs) in Aedes aegypti Reproduction Through RNA Interference-Mediated Downregulation
… included RNAi-mediated knockdown of DP-1, accompanied by evaluations using quantitative PCR (qPCR), Western blotting (WB), co-immunoprecipitation (Co-IP), follicle length measurement, and egg counting to assess the role of DP-1 in reproductive functions. For the first time, the inhibition of …
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The Interaction of the Human Adenovirus E1A Protein with the Human DREF Transcription Factor
… to modulate host and viral transcription. The second is to induce host cell cycle progression to S phase, to promote an optimal environment for viral replication. E1A performs its functions by binding and manipulating over 50 cellular factors. Interestingly, I found that E1A is capable of …
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Discovering LC3-interacting region (LIR) motifs in hemorrhagic fever viruses: Implications for host autophagy and viral replication.
… (HFVs) pose significant global health and economic burdens, yet limited FDA-approved therapeutics exist to combat their spread. Determining alterations in host processes during infection can enhance our understanding of viral replication and lead to potential host-based therapeutic targets. …
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Investigation of Vesicular Stomatitis Virus Interaction with Host Cell Proteins and Associated mRNA Structures
… proven to be an effective vaccine vector and oncolytic therapy agent. For these reasons, the interaction between VSV and host cells in the context of infection and innate immunity was of great interest. Previous work performed by the laboratory of Douglas Lyles determined that a class of …
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