Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"Co-IP"”.
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Interactions between IP3 receptor and Kras-induced actin-interacting protein
Inositol 1,4,5-trisphosphate receptors (IP3R) function as intracellular channels, facilitating the release of Ca2+ from the endoplasmic reticulum (ER) upon IP3 binding. Before responding to IP3 in intact cells, IP3R must undergo a process called 'licensing,' involving the association with …
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A link between c. Elegans morphogenesis and mrna export
Multiple studies have explored the mechanisms governing development of the Caenorhabditis elegans pharynx. In this study we used whole genome sequencing (WGS) and CloudMap/Hawaiian Variant Mapping to specifically map a mutation that produced a Pun pharynx (unattached pharynx) phenotype. We …
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Myxoid Liposarcoma: Models and Mechanisms of Sarcomagenesis and Response to Radiation Therapy
<p>Myxoid liposarcoma (MLPS) is a malignant soft tissue sarcoma characterized by a pathognomonic t(12;16)(q13;p11) translocation that produces a fusion oncoprotein, FUS-CHOP. This cancer is remarkably sensitive to radiotherapy and exhibits a unique pattern of extrapulmonary metastasis. However, the …
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Regulation of Antimicrobial Peptide Genes in the Tobacco Hornworm Manduca sexta
… as natural or non-specific immune system, is conserved from insects to humans. The insect innate immune system is composed of humoral and cellular components. Insect humoral responses mainly include synthesis of antimicrobial peptides (AMPs) and activation of the prophenoloxidase (PPO) system. …
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Elucidating the Function of Krüppel Homolog 1 (Kr-h1) Associated Proteins (KAPs) in Aedes aegypti Reproduction Through RNA Interference-Mediated Downregulation
The transcription factor Krüppel homolog 1 (Kr-h1) is crucial in multiple reproductive processes of Aedes aegypti mosquitoes, including previtellogenesis, vitellogenesis, and oogenesis. This study explores the interaction between Kr-h1 and its potential associated proteins (KAPs), with a specific …
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Investigation of Vesicular Stomatitis Virus Interaction with Host Cell Proteins and Associated mRNA Structures
… proven to be an effective vaccine vector and oncolytic therapy agent. For these reasons, the interaction between VSV and host cells in the context of infection and innate immunity was of great interest. Previous work performed by the laboratory of Douglas Lyles determined that a class of …
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The Role Of The DOMAIN OF UNKNOWN FUNCTION (DUF2358) In Sugar Signalling Pathways In Arabidopsis thaliana
… as drought and high salinity . This study was conducted to investigate the role of DUF2358 in sugar signalling pathways under stress conditions which affect primary metabolism. For that, we investigated the role of DUF2358 in stress signaling pathways using knockout and overexpress mutants. …
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Genetic Approach to Discover ARMC4 as a Novel NF-κB Negative Regulator and Tumor Suppressor in Colorectal Cancer
… a powerful technique established by us, we discovered armadillo repeat containing protein 4 (ARMC4) as a novel negative regulator of NF-κB in colorectal cancer (CRC). ARMC4 is a rarely studied protein only known to date for its role in primary ciliary dyskinesia (PCD) and mouse spermatogenesis. …
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Investigating the role of miR-21 in autophagy and VMP1
… around the miR-21/VMP1 locus in IBD patients compared to HC and elevated levels of VMP1 and miR-21 have been detected in patients. MiR-21 is a microRNA that is associated with immune related disorders, and VMP1 Is a key regulator of autophagy, a pathway heavily implicated in CD pathogenesis. …
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Molecular Physiology of the Chlamydomonas Pyrenoid
Operation of the CO<sub>2</sub>-concentrating mechanism (CCM) in the model green alga *Chlamydomonas reinhardtii* relies on confining Rubisco in a proteinaceous microcompartment, the chloroplastic pyrenoid. Despite a long history of research, this enigmatic structure lacks a full definition both in …
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Identification of the Pokeweed Antiviral Protein Interactome by Co-Immunoprecipitation-Mass Spectrometry
Ribosome-inactivating proteins (RIPs) are produced primarily by plants and are named for their enzymatic ability to depurinate ribosomal RNA. RIPs have been shown to have antiviral, antifungal, and antibacterial activity in vitro and when expressed transgenically. They are therefore of interest for …
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Ehrlichia chaffeensis E3 Ligase TRP120 Ubiquitinates Tumor Suppressor FBW7 for Degradation to Promote Infection
… (E. chaffeensis) exploits evolutionarily conserved Notch and Wnt host cell signaling pathways to downregulate innate immune host defenses and promote infection. The multifunctional E. chaffeensis TRP120 effector which has HECT E3 ubiquitin ligase activity, interacts with the host nuclear …
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Involvement of Scribble protein in breast cancer invasion and metastasis
A common feature of cancer cells is the loss of cell polarity. Scribble is a cell polarity protein with an unknown mechanism for its role in tumour suppression. Changes in the phosphorylation pattern of four serine sites at the C-terminal of scribble, implicate scribble in breast cancer invasion …
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Characterisation of potential regulators of PAMP-triggered immunity
… (PAMPs) through surface-localised pattern-recognition receptors (PRRs). EFR and FLS2 are well characterised PRRs, which recognise the bacterial elongation factor Tu and flagellin, respectively. A variety of downstream responses upon PAMP perception have been described. However, it is still …
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Co-regulation of the Smooth Muscle Cell Alternative Splicing Programme by the RNA-Binding Proteins RBPMS, RBFOX2, MBNL1, and QKI
… of vascular smooth muscle cells (VSMCs) from a contractile /differentiated state to a synthetic/ proliferative state is a hallmark of many cardiovascular diseases, driven in part by extensive alternative splicing (AS) regulation. RBPMS, a master splicing regulator in differentiated VSMCs, is …
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The Secretory Pathway Calcium ATPase 2C promotes increased cytosolic calcium levels
… SPCA2C was expressed in HEK293A cells. Co-immunofluorescence (Co-IF) determined subcellular localization. Using Fura2 imaging, cytosolic Ca2+ concentration [Ca2+] was examined during rest, SOCE and secretagogue-stimulated signaling. Effects of SPCA2C over-expression were also examined in …
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Controlling Pseudomonas aeruginosa Persister Cells by Human Granulocyte Macrophage Colony-Stimulating Factor
… state when the antibiotic treatments are discontinued. During bacterial invasions, the host immune cells secrete special signaling proteins, known as cytokines which orchestrate events leading to human immune response and elimination of bacterial pathogens. However, compared to the well …
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Molecular mechanisms involved in the regulation of beta-cell viability by Signal Regulatory Protein-Alpha (SIRPα)
… cytokine pathways, acting via STAT1 and STAT2, contribute to β-cell death whereas anti-inflammatory mechanisms (operating via cytokines such as Interleukin-13 (IL-13)) are downregulated. IL-13 has been shown to protect β-cell viability via STAT6 signalling, and preliminary data suggested that …
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Regulation of Gene Expression in Trypanosoma brucei
… divergent well-studied eukaryotes and many discoveries of general interest have been made in T. brucei. Atypical for a eukaryote, in T. brucei genes transcribed by RNA polymerase II (RNA pol II) are arranged in polycistronic transcription units (PTUs). mRNAs are separated post-transcriptionally …
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The Role of ATP and Cleavage Factor Phosphorylation in pre-mRNA 3' Cleavage Reaction
… a functional messenger RNA (mRNA) transcript. It is long-known that ATP can significantly stimulate the in vitro cleavage of adenovirus type 2 L3 (Ad2L3) RNA substrate. Here, we used ATP analogs in structure-activity assays to show that the structural features of ATP and its analogs …
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