Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"Clustered regularly-interspaced short palindromic repeats (CRISPR/Cas9)"”.
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Development and application of a general method for somatic cell genetics
… however, prevailing screening methods fall short - leaving our understanding of human genes incomplete. Here, I describe the development of a general method for genetic screening in mammalian cells using the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system. In …
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Development of an inducible transcriptional control system in plasmodium falciparum with applications to targeted genome editing
… able to use this expression system to adapt a clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system in the parasite and use it to achieve genome editing at high efficiency at multiple loci. The data imply an adaptable system to readily edit the genome of the parasite …
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Recombinant Engineering Strategies for the Production of Therapeutic Coagulation Factor Proteins
… activation system designed around the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas9 nuclease. Utilizing a plasmid-based strategy to express the CRISPR/Cas9-SAM system components our study validated the compatibility of the system with human cells, confirmed its …
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Understanding antigen processing in chickens using genome editing technology
… In this thesis, the genome editing approach of clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 was used to begin to understand the peptide loading systems in the chicken. For the first time, the CRISPR-Cas9 system was optimised and implemented in avian cell lines, …
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Engineering Saccharomyces cerevisiae with enhanced supply of precursor metabolites for efficient production of fuels and chemicals
Made available in DSpace on 2015-09-29T21:03:11Z (GMT). No. of bitstreams: 2 LIAN-DISSERTATION-2015.pdf: 6484498 bytes, checksum: dfafbe7074729b6385dc6f63b3a93fa9 (MD5) LICENSE.txt: 4210 bytes, checksum: 61fac6473db07dd95d79ef7c7ac61659 (MD5) Previous issue date: 2015-07-14
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Multiplex genome editing and gene regulation in eukaryotic cells
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2019-05-01
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Natural product discovery and engineering at the protein, pathway, and genome scales
… and improve production. Here, we show that the CRISPR/Cas9 system of S. pyogenes can be reconstituted in S. lividans for high-efficiency, multiplex editing of genomic loci. We show that deletions ranging from 20 bp to 31 kb can be successfully introduced in one step, and extend this approach to …