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Showing 1 to 20 of 50 for “"Clustered Regularly Interspaced Short Palindromic Repeats"”.

  1. Analysis of the role of nuclear factor-kappa B in insulin resistance caused by antiretroviral drugs

    University of Pretoria2020

    … however led to insulin resistance amongst users. Clustered regularly interspaced short palindromic repeats (CRISPR) CRISPR-associated nuclease 9 (Cas) has been used to knockout NFκB to understand the pathway at which antiretroviral treatment causes insulin resistance. Heteroduplex mobility assay …

    pretoria Repository record

  2. Identification of genotype-specific dependencies in Keap1-deficient lung adenocarcinoma

    Romero, Rodrigo,Ph. D.Massachusetts Institute of Technology.Massachusetts Institute of Technology2019

    … of somatic genome editing provided by clustered regularly interspaced short palindromic repeats (CRISPR) and the endonuclease, CRISPR associated protein 9 (Cas9) has accelerated our ability to functionally interrogate putative genetic drivers in the context of mammalian cells and …

    mit Repository record

  3. Decipher in situ signaling and complex genetics with cellular recording and combinatorial perturbations

    Cui, Cheryl H. (Cheryl Hao)Massachusetts Institute of Technology2017

    … cellular behaviors. The recent discovery of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated (Cas) system from prokaryotic adaptive immune system demonstrated unprecedented genome editing efficiency and programmability in sequence specific genome editing of …

    mit Repository record

  4. CRISPR3 Regulates Exopolysaccharide Production in Myxococcus xanthus

    Wallace, Regina A.Virginia Tech2013

    … and Type IV pili (T4P), harbors three Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) on its chromosome. CRISPR systems, which function as an adaptive immune system in prokaryotes, are classified into three types based on CRISPR-associated genes. Evidence suggests …

    vt Repository record

  5. Mechanisms of bacterial CRISPR immunity and a phage counter-defense

    Dillard, Kaylee ElizabethThe University of Texas at Austin2021

    … of defense is the adaptive immune system CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats). Upon infection, the bacterial host uptakes a genetic memory from the bacteriophage to incorporate into its genome. This molecular memory is used to target CRISPR-associated (Cas) proteins …

    texas Repository record

  6. Discovery and characterization of Cas13b, a differentially regulated RNA-targeting CRISPR system

    Smargon, Aaron AndrewMassachusetts Institute of Technology2018

    … RNA in a simple, efficient manner. CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins) systems, which confer adaptive immunity to prokaryotes, have revolutionized DNA targeting through the engineering of RNA-programmable Cas9-based tools. …

    mit Repository record

  7. An integrated CRISPR-Cas toolkit for engineering human cells

    Perli, Samuel DavidMassachusetts Institute of Technology2015

    Natively functioning Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated (Cas) system is a prokaryotic adaptive immune system that confers resistance to foreign genetic elements including plasmids and phages. Very recently, a two-component CRISPR-Cas technology from …

    mit Repository record

  8. Investigating Hybrid Cyclic/Linear and Linear Peptides as Vehicles for Nucleic Acid Delivery

    Alhazza, Abdulelah2024

    <p>Small interfering RNA (siRNA) and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated Protein 9 (Cas9) are advanced biotechnological approaches that have been able to temporarily downregulate gene expression through post-transcription RNA interference or …

    chapman Repository record

  9. Investigating the transcriptional regulation of BCL11A in Triple-negative Breast Cancer

    Tsang, Nicola Hoi YeeUniversity of Cambridge2019

    … a novel proteomic approach utilising the Clustered regularly interspaced short palindromic repeats – CRISPR associated 9 (CRISPR-Cas9) technology, to identify putative transcriptional regulators of BCL11A in TNBC. The first step of this project involves the identification of regulatory …

    cambridge Repository record

  10. Identification of novel genetic vulnerabilities and therapeutic targets in acute myeloid leukaemia using CRISPR dropout screens

    TZELEPIS, KONSTANTINOSUniversity of Cambridge2017

    … targets in AML, I have optimized a genome-wide clustered regularly interspaced short palindromic repeats (CRISPR) screening platform and use it to identify genetic vulnerabilities in AML cells. This work led to the identification of 492 AML-specific cell-essential genes, including several …

    cambridge Repository record

  11. Cell-free synthetic biology for affordable, on-demand diagnostics

    Dy, Aaron J.(Aaron James)Massachusetts Institute of Technology2019

    … Finally, I present work to develop and use clustered regularly interspaced short palindromic repeats (CRISPR) enzyme-based diagnostics to achieve attomolar sensitivity and single-nucleotide mismatch specificity. Together, these projects demonstrate a set of advancements in cell-free …

    mit Repository record

  12. Prevention of Duchenne Muscular Dystrophy by CRISPR/Cas Therapeutic Genome Editing

    Zhang, Yu2020

    … for this debilitating disease. The CRISPR/Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins) was first discovered as an adaptive immune system in bacteria and archaea for defending against phage infection. Recently, the CRISPR/Cas system has been …

    utswmed Repository record

  13. Targeted destruction of intracellular DNA using a CRISPR-based genetic device that can be carried indefinitely in the host genome

    Caliando, Brian JamesMassachusetts Institute of Technology2015

    … based on the organism's native type-IE CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) DNA interference (DNAi) pathway. The optimized DNAi device is comprised of two components: a chromosomally-integrated actuator element, which encodes the minimal set of CRISPR-associated …

    mit Repository record

  14. CRISPR AS A TOOL FOR TARGET DISCOVERY AND TECHNOLOGICAL INNOVATION: HIGH-THROUGHPUT CBE SCREENING TO EVALUATE M6A MODIFICATION PATHWAY IN CANCER PROLIFERATION AND DRUG RESISTANCE, AND INNOVATIVE PRIME EDITING STRATEGIES FOR DISEASE MODELLING AND THERAPY

    SCICHILONE, MARTINAUniversità degli Studi di Milano2026

    … to this treatment in AML. In this work, we used Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Cytosine base editing (CBE) screening to perform saturating mutagenesis of m6A-pathway effectors. In details, we assessed the effect of single nucleotide variants (SNVs) in CRC …

    milano Repository record

  15. Genomic diversity and genomic typing of Salmonella enterica serovar Typhimurium

    Fu, SongzheUNSW, Sydney2017

    … 15 repeat groups (RG) primarily determined by clustered regularly interspaced short palindromic repeats (CRISPR) typing. Nine Australian strains previously sequenced and 57 strains from publicly available sequenced strains isolated from different countries were included in the analysis. …

    unsw Repository record

  16. Development and application of a general method for somatic cell genetics

    Wang, TimMassachusetts Institute of Technology2017

    … however, prevailing screening methods fall short - leaving our understanding of human genes incomplete. Here, I describe the development of a general method for genetic screening in mammalian cells using the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system. In …

    mit Repository record

  17. Development of an inducible transcriptional control system in plasmodium falciparum with applications to targeted genome editing

    Wagner, Jeffrey C. (Jeffrey Charles)Massachusetts Institute of Technology2014

    … able to use this expression system to adapt a clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system in the parasite and use it to achieve genome editing at high efficiency at multiple loci. The data imply an adaptable system to readily edit the genome of the parasite …

    mit Repository record

  18. IDENTIFICATION OF FACTORS INVOLVED IN DOUBLE STRANDED RNA-INDUCED CELL DEATH THROUGH GENOME-WIDE CRISPR SCREENS

    TRASTULLI, DEBORAHUniversità degli Studi di Milano2023

    … strategies, we performed a Genome-wide clustered regularly interspaced short palindromic repeats (CRISPR) screening and integrative analysis to prioritize gene targets that favour/inhibit dsRNA-dependent cell death. The screen revealed that RNA surveillance, RNA Polymerase II …

    milano Repository record

  19. Exploring Non-transgenic CRISPR-Cas9 gRNA Delivery Using Transactivation in Nicotiana benthamiana

    Garland, SarahUniversity of Cambridge2020

    Gene editing via CRISPR-Cas9 (Clustered, Regularly Interspaced, Short Palindromic Repeats – CRISPR-associated protein 9) is a powerful tool in biotechnology. The method involves an endonuclease Cas9 forming a complex with a guide RNA (gRNA) that matches a distinct DNA target sequence, causing …

    cambridge Repository record

  20. Generation of a CRISPR-Cas9 mediated knock-in reporter for the GRIA3 candidate gene for schizophrenia

    Breen, LisaUniversity College Cork2022

    … project aimed to modify the cell line using a Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) - CRISPR associated protein 9 (Cas9) homology directed repair (HDR) approach so that a donor reporter vector containing the Gaussia secreted luciferase gene and linked green …

    cork Repository record

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