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Showing 1 to 14 of 14 for “"Clostridium thermocellum"”.
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Regulation of cellulase activity and synthesis in Clostridium thermocellum
Thesis (Sc.D.)--Massachusetts Institute of Technology, Dept. of Nutrition and Food Science, 1984.
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SAXS and X-ray Crystallography Studies of the Cellulosome from Clostridium thermocellum
… structural studies of the cellulosome from Clostridium thermocellum. We first describe the crystal structure of a heterodimeric complex comprising the type-II cohesin (CohII) from cell surface anchoring protein SdbA and a trimodular C-terminal truncation of the CipA scaffoldin protein …
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Direct conversion of cellulosic biomass to ethanol by mixed culture fermentation of Clostridium thermocellum and Clostridium thermosaccharolyticum
Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Nutrition and Food Science, 1982.
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The physiology of Clostridium thermocellum : the toxicity of its fermentation products in relation to the energy metabolism
Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Nutrition and Food Science, 1981.
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Ethanol Production from Timothy (Phleum pratense L.)
… notaðar voru til etanól framleiðslunnar voru: Clostridium thermocellum, Thermoanaerobacter ethanolicus, Thermoanaerobacter stofn J1, Zymomonas mobilis, Kluyveromyces marxianus og Saccharomyces cerevisiae. Vallarfoxgrasið var slegið sumarið 2014 á Möðruvöllum í Hörgárdal. Borin voru saman fjögur …
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Enzymatic Production of Cellulosic Hydrogen by Cell-free Synthetic Pathway Biotransformation(SyPaB)
… phosphorylase, were cloned from a thermophile Clostridium thermocellum, and heterologously expressed in Escherichia coli, purified and characterized in detail. Finally, up to 93% of hydrogen was produced from cellulosic materials (11.2 mol H2/mol glucose equivalent). A nearly 20-fold …
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Cell-Free Biosystems Comprised of Synthetic Enzymatic Pathways: Development of Building Blocks, Immobilization of Enzymes, Stabilization of Cascade Enzymes, and Generation of Hydrogen
… maritime, phosphoglucose isomerase (PGI) from Clostridium thermocellum, triose phosphate isomerase (TIM) from Thermus thermophiles and fructose bisphosphate aldolase (ALD) from T. maritima and T. thermophilus. The recombinant proteins were over-expressed in E. coli, purified and characterized. …
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Molecular analysis of two cellulase genes from Ruminococcus flavefaciens FD-1 and their transcriptional regulation
… It showed 40% identity with endoglucanase C of Clostridium thermocellum and 27.4% identity with endoglucanase 3 of Fibrobacter succinogenes. These three enzymes are grouped into subfamily "A3". The ATG start codon of celA is preceded by a GAGG sequence, predicted to be a ribosome binding site. …
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Cellulases from extremely thermophilic anaerobic bacteria: a comparison of several new cellulolytic isolates and the partial purification and characterisation of components of the cellulase complex from one isolate
… supernatants were between 10 and 35% of those of Clostridium thermocellum grown on the same medium (but at 60°C). All produced acetic acid and ethanol in varying proportions, with a consequent tendency to decrease the pH of the growth medium. Cellobiose at 0.2%(w/v) repressed production of CMCase …
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Structure and Mechanism of PCAT1, a Polypeptide Processing and Secretion Transporter
… I determined the structure of a PCAT from Clostridium thermocellum (abbreviated as PCAT1) in the substrate-bound inward-facing conformation. I have identified structural features that enable the substrate to bind, translocate into the transmembrane cavity, and orient properly for cleavage. …
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Discovery and characterization of novel RNA repair systems
… and the N-terminal half of Hen1 (Hen1-N) from Clostridium thermocellum, and provided the molecular basis for the ligase activation of bacterial Pnkp by Hen1. We also carried out a detailed functional study to pinpoint the activation step during RNA ligation. Guided by the sequence and …
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ISPH fusion proteins, ISPH inhibition, and bisphosphonate mechanism of action
… obligate anaerobic bacteria. An IspH-RPS1 from Clostridium thermocellum was cloned, expressed, and found to be catalytically active. Mutagenesis studies showed that IspH catalytic activity was independent of the number of RPS1 domains fused to the IspH domain. The sequences being from obligate …
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Construction of plasmid-based expression and secretion vectors and study of the immobilization of proteins on the surface of Bacillus subtilis cells
… of amyQ amylase and cellulase A and B of Clostridium thermocellum was demonstrated. Gram-positive bacteria code for one or more enzymes termed sortases that catalyze the covalent anchoring of substrate proteins on their cell wall. They recognize an amino acid sequence designated sorting …