Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 27 for “"Clostridium beijerinckii"”.
-
Enzymology of butanol formation in Clostridium Beijerinckii
The present study encompasses an investigation of the expression of solvent forming enzymes and purification and characterization of butanol-forming enzymes. More sensitive and accurate procedures for the determination of acids and solvents in cultures have been developed, which led to the …
-
Nitrogen Metabolism and Solvent Production in Clostridium Beijerinckii Nrrl B593
… several solvent-producing clostridia, including Clostridium acetobutylicum and Clostridium beijerinckii, are nitrogen-fixing organisms and both nitrogen-fixation and alcohol production (n-butanol, isopropanol and ethanol) are reductant-dependent processes, the effect of nitrogen fixation on the …
-
Investigation of carbon catabolite repression in Clostridium beijerinckii NCIMB 8052
… ccpA mutant strain. The sucrose operon from C. beijerinckii NCIMB 8052, scrARBK, has been characterised at the physiological and genetic levels with the ScrR repressor found to negatively auto-regulate the operon.
-
Differential Protein Expression and Butanol Production using <i>Clostridium beijerinckii</i>
… per gallon. Our research uses the bacterium <i>Clostridium beijerinckii</i> as the model for the fermentative production of butanol using discarded lignocellulose from biomass, specifically wood. The wood biomass (wood chip), is processed by treatment with high temperature and pressure, …
-
Genetic and Physiological Characterization of Clostridium Beijerinckii BA101 and NCIMB 8052
… also able to stabilize solvent production by C. beijerinckii BA101, a hypersolvent producing mutant derived from NCIMB 8052. Moreover, BA101 demonstrated a greater increase in solvent production than NCIMB 8052 when acetate was added into MP2 medium. During the exponential growth phase, the …
-
Studies on the sucrose utilization system of Clostridium beijerinckii NCIMB 8052
… of the sucrose utilization system of Clostridium beijerinckii NCIMB 8052. The C. beijerinckii sucrose utilization operon, scrARBK, was cloned in three stages. In the first stage a C. beijerinckii genomic library was screened in Escherichia coli for clones able to utilize sucrose. Using …
-
Acetone, Butanol, and Ethanol (ABE) Production from Food Waste via Clostridium beijerinckii
… fermentation of these wastes was performed using Clostridium beijerinckii via batch fermentations. Correlations of ABE and butanol yields with the individual components of food waste composition were performed to better understand which components are key to ABE fermentation. Overall, this study …
-
A systems biology approach to understanding and improving butanol production in clostridium beijerinckii
… and potential fuel additive or replacement. Clostridium beijerinckii is an attractive microorganism for butanol production because it (1) naturally produces the highest recorded butanol concentrations as a byproduct of fermentation; and (2) can co-ferment pentose and hexose sugars (the …
-
Increasing fermentative butanol production in Clostridium beijerinckii using oxidized extracellular electron shuttling molecules
… shuttles to alter the metabolic pathways in Clostridium beijerinckii NCIMB 8052 fermentation to increase butanol yield with glucose as a primary substrate. 1-5mM anthraquinone-2,6-disulfonate (AQDS), the oxidized form of the electron shuttle, were introduced into batch cultures of C. …
-
Nitrogen metabolism and butanol production by South African clostridium beijerinckii and clostridium saccharobutylicum strains
… industry. A collection of 19 solventogenic Clostridium beijerinckii and 11 Clostridium saccharobutylicum strains isolated from the National Chemical Products (NCP) ABE fermentation plant in Germiston, South Africa, were classed according to species by a quick species-specific colony PCR and …
-
The Cloning of a Putative Regulatory Gene and the sol Region from Clostridium beijerinckii
… reliable. From an isopropanol-producing strain Clostridium beijerinckii NRRL B593, a gene which encodes a putative NtrC-like regulatory protein was cloned and sequenced. The gene codes for a polypeptide of 632 amino acids and has been designated the stc gene. Expression of the stc gene was …
-
Metabolic network reconstruction and genome-scale model of butanol-producing strain clostridium beijerinckii ncimb 8052
… and potential fuel additive or replacement. C. beijerinckii is an attractive microorganism for strain design to improve butanol production because it (i) naturally produces the highest recorded butanol concentrations as a byproduct of fermentation; and (ii) can co-ferment pentose and hexose …
-
Production of 1-butanol and butanol isomers by metabolically engineered Clostridium beijerinckii and Saccharomyces cerevisiae
… fermentation by solventogenic clostridia such as Clostridium beijerinckii. Although the ABE fermentation is considered as a promising process for butanol production, current drawbacks in the ABE fermentation include low titer, yield, and productivity of solvent production that needs to be improved …
-
Examination of Glucose Transport in Clostridium Beijerinckii NCIMB 8052 and the Solvent Hyper-Producing Mutant BA101
Glucose uptake and accumulation by Clostridium beijerinckii BA101, a butanol hyper-producing mutant was examined during various stages of growth. Glucose uptake in C. beijerinckii BA101 was repressed 20% by 2-deoxyglucose (2-DG) and 25% by mannose, while glucose uptake in C. beijerinckii 8052 was …
-
Molecular Characterization and Utilization of the CAK1 Filamentous Viruslike Particle Derived From Clostridium Beijerinckii NCIMB 6444
… Thermoanaerobacterium polysaccharolyticum in C. beijerinckii NCIMB 8052 and the sol operon of C. acetobutylicum DSM 792 in C. beijerinckii SA-2 when using pYL102E indicates that pYL102E can be used as an E. coli-C beijerinckii shuttle vector. This observation indicates the utility of CAK1-derived …
-
Reduced extracellular electron shuttles increase hydrogen production and substrate utilization in pure culture Clostridium beijerinckii fermentation
… in a model hydrogen producing microorganism-Clostridium beijerinckii fermentation system. Results show that adding extracellular hydroquinones (anthrahydroquinone disulfonate; AH2QDS) increased the hydrogen yield by 24-37% and the extent of xylose utilization by up to 56% when xylose was the …
-
CoA-transferase and 3-hydroxybutyryl-CoA dehydrogenase: acetoacetyl-CoA-reacting enzymes from Clostridium beijerinckii NRRL B593
… homogeneity from the solvent-producing anaerobe Clostridium beijerinckii strain NRRL B593. The identities of 34 of the 35 N-terminal amino acid residues have been determined. The enzyme exhibited a native M<sub>r</sub> of 213,000 and a subunit M<sub>r</sub> of 30,800. It is specific for the …
-
Studies on the regulation of nitrogen metabolism in clostridium acetobutylicum NCP 262 and clostridium beijerinckii NCIMB 8052
In order to produce Clostridium acetobutylicum strains with improved solventogenic capabilities through genetic manipulation, a thorough understanding of the mechanisms governing solvent production is required. Nitrogen metabolism has been shown to be important in the regulation of sporulation and …
-
Transcriptomic analyses of Clostridium beijerinckii NCIMB 8052 during transition from acidogenesis to solventogenesis and under butyrate supplemented conditions
… value as an industrial chemical feedstock. Clostridium beijerinckii is among the prominent species for biobutanol production as it demonstrates a broad substrate range for growth and solvent production. Although the transcriptome structure and transcriptional profiling are essential for …
-
The characterization of Clostridium beijerinckii NRRL B592 cells transformed with plasmids containing the butanol-production genes under the control of constitutive promoters
Clostridium beijerinckii is a spore-forming, obligate anaerobe that is capable of producing butanol, acetone and isopropanol. These industrial chemicals are traditionally known as solvents. The regulation of solventogenic fermentation is linked to the onset of sporulation, so that by the time the …
Page 1 of 2