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Showing 1 to 20 of 39 for “"Clostridium acetobutylicum"”.
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Genetic studies on Clostridium acetobutylicum
… of the cellulolytic properties of Clostridium acetobutylicum and the development of a genetic transfer system for this organism. The production of a carboxymethyl cellulose and a cellobiase by C acetobutylicum was demonstrated. In liquid medium the carboxymethyl cellulase was …
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Hydrogenase of Clostridium acetobutylicum ATCC 824
C. acetobutylicum is an anaerobic bacterium that produces acetic and butyric acids, hydrogen gas, and carbon dioxide during the exponential phase of growth. When the culture pH is allowed to remain near 4.5, the metabolism switches to the production of the neutral compounds (solvents) - acetone, …
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Regulation of clostridium acetobutylicum glutamine synthetase glnA gene
… in the Gram positive anaerobic bacterium Clostridium acetobutylicum, which has been utilized in industrial fermentations to produce acetone, butanol and ethanol. The C. acetobutylicum glutamine synthetase (GS) glnA gene region originally cloned in Escherichia coli, is characterized by the …
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Assembling improved gene annotations in Clostridium acetobutylicum with RNA sequencing
The C. acetobutylicum genome annotation has been markedly improved by integrating bioinformatic predictions with RNA sequencing(RNA-seq) data. Samples were acquired under butanol, butyrate, and unstressed treatments across various growth stages to sample the transcriptome from a range of …
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Molecular genetic characterization of two solvent pathway dehydrogenases from Clostridium Acetobutylicum
Clostridium acetobutylicum P262 is an endospore-forming Gram-positive anaerobic bacterium, which has been used for the industrial production of acetone and butanol from carbohydrate substrates. This study forms part of a wider research effort into the genetics and molecular biology of C. …
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Studies on nucleotide levels and electron transport genes of Clostridium acetobutylicum P262
Clostridium acetobutylicum P262 is an endospore-forming Gram positive anaerobic bacterium, and for many years this organism has been used in the industrial fermentation for the production of acetone and butanol from carbohydrate substrates. The aims of this thesis included studies on small …
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A cluster of two serine transfer RNA genes from Clostridium acetobutylicum P262
… host, was previously established to clone C. acetobutylicum P262 electron transport genes which may play a role in solvent metabolism in this bacterium. In theory, metronidazole would be reduced under anaerobic conditions to a cytotoxic intermediate by C. acetobutylicum P262 electron transport …
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Expression of a clostridium acetobutylicum endoglucanase and an endoxylanase in saccharomyces cerevisiae
S. cerevisiae is widely used in industrial processes, in particular ethanol production. The aim of this study was to examine the feasability of cloning lignocellulase genes into yeast. These enzymes could possibly extend the substrate range of S. cerevisiae, thereby possibly making the process of …
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Characterization of a transposon-induced pleiotropic metronidazole resistant mutant of Clostridium acetobutylicum P262
… transport gene(s) from the solvent-producing Clostridium acetobutylicum strain P262, using transposon-induced metronidazole resistance as a selection system. In the process, the feasibility of transposon mutagenesis in this strain, which lacks conventional systems for DNA delivery, was …
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Regulation and characterization of the nitrogen assimilatory gene cluster in Clostridium acetobutylicum P262
The solventogenic Clostridium acetobutylicum strain P262 was used for the commercial production of solvents in South Africa from 1945 to 1983 (Jones and Woods, 1986a). Our laboratory has focused on understanding two: fundamental aspects of its physiology, namely nitrogen metabolism and electron …
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Production of acetone and butanol by Clostridium acetobutylicum using free and immobilized cells
Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Nutrition and Food Science, 1982.
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Studies on the regulation of solvent production and endospore formation in Clostridium Acetobutylicum P262
… solventogenesis and endospore formation in Clostridium acetobutylicum strain P262. Growth and endospore formation was monitored in a number of complex and minimal media and as a result of these studies a new defined sporulation medium was developed. The defined system produced high levels of …
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Molecular characterization of three linked genes, fixB, βshbd and adh1 from Clostridium acetobutylicum P262
Clostridium acetobutylicum P262 is an endospore-forming Gram-positive anaerobic bacterium, and it has been used in the industrial production of acetone and butanol for many years. The aim of this study was to characterize the upstream region of the βhbd gene which is linked to the adh1 gene, and to …
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Biochemistry and physiology of Clostridium acetobutylicum relative to its fermentation shift and to culture degeneration
… associated with the fermentation shift in C. acetobutylicum using a wild-type strain, ATCC 824, and several strains defective in solvent production (a butanol tolerant, low butanol producing derivative, SA-2; a butanol dehydrogenase minus derivative, AA-115; a degenerative derivative, DG; and …
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A reassessment of the production of acetone and butanol by Clostridium acetobutylicum in continuous culture
The production of acetone and butanol by Clostridium acetobutylicum P 262 was studied in continuous culture under conditions where the nutrients were present in excess of the requirements and the cell growth was limited by the products formed during the fermentation. This system differs from most …
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Studies on Clostridium acetobutylicum P262 : sporulation induction and analysis of an oligosporogenous, solvent-deficient mutant
… to design a sporulation induction medium for Clostridium acetobutylicum P262. Of acetic acid, butyric acetone and butanol, was found to be most effective at induction. Induction was more efficient at low pH values under certain conditions. The heat resistance of mature spores was determined …
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Genetic enhancement, regulation, cellular localization and purification of amylolytic enzymes produced by Clostridium acetobutylicum ATCC 824
Two types of mutants of Clostridium acetobutylicum were isolated. The amylolytic enzyme synthesis in C. acetobutylicum BA 101 and BA 105 was amplified and catabolite de-repressed, respectively, as compared to the parental ATCC 824 strain.
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