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Showing 1 to 6 of 6 for “"Clostridium Scindens"”.
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Development of a Gene Transfer System in Clostridium Scindens VPI 12708
Clostridium scindens VPI 12708 (previously known as Eubacterium sp. VPI 12708) is a bile-acid dehydroxylating bacterium originally isolated from the feces of a colon cancer patient. Many genes required for bile acid 7-a dehydroxylation are found on a large bile acid inducible operon (bai) that has …
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Comparative genome analysis of strains of clostridium scindens: A bile acid and cortisol metabolizing gut bacterium
Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2025-02-04 without embargo terms
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Adding Upstream Sequence and a Downstream Reporter to the Bile Acid Inducible Promoter of <I>CLOSTRIDIUM scindens</I> VPI 12708
… is the bile acid inducible operon, for which <i>Clostridium scindens </i>VPI 12708 serves as the model organism. There is a lack of knowledge on how this operon is regulated, so the goal of this project was to create a genetic construct consisting of upstream regulatory elements, a bile acid …
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ENZYMOLOGY AND MOLECULAR BIOLOGY OF BILE ACID 7alpha- AND 7beta- DEHYDROXYLATION BY THE INTESTINAL BACTERIA CLOSTRIDIUM SCINDENS AND CLOSTRIDIUM HYLEMONAE
… in a small number of organisms within the genusClostridium. The biochemistry of this pathway has been largely worked out. The third step in the pathway is introduction of a delta-4-double bond; however, the gene product(s) responsible have not been identified. The baiCD and baiH genes were …
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Characterization of enzymatic pathways involved in cortisol and bile acid metabolism by the gut microbiota
… enzymes encoded by the desA and desB genes from Clostridium scindens ATCC 35704. Steroid-17,20-desmolase is responsible for the side chain cleavage that biotransforms cortisol into 11β-hydroxyandrostenedione (11β-OHAD). The reaction performed by the steroid-17,20-desmolase appears to be regulated …
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Steroid metabolism in the human gut by microbial hydroxysteroid dehydrogenases
… were identified in the human gut microbes Clostridium scindens ATCC 35704, C. hylemonae DSM 15053, and Peptacetobacter hiranonis DSM 13275. After cloning and overexpression of these enzymes in Escherichia coli, biochemical analysis revealed a preference for the oxo-intermediate, …