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Showing 1 to 15 of 15 for “"Clonogenic assay"”.
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Effects of Green Tea Extract (GTE) for Normal and Cancer Cell Death by Radiation
… 농도의 녹차추출물을 단독으로 처리하거나 녹차추출물 처리 후 방사선을 조사하고 MTT assay, clonogenic assay, ROS assay를 시행하였다. 방사선과 함께 0.025 및 0.05 mM의 녹차추출물을 사용하였을 때 방사선만을 조사받은 경우에 비해 정상 세포주인 IEC-6에서 방사선조사 후 세포생존율이 증가하였으며 방사선조사를 받지 않은 경우에도 같은 비율로 세포생존율의 증가가 있었다. 대장암 세포주인 HCT-116에서는 0.025 및 0.05 mM 녹차추출물 처리로 방사선에 의한 세포살상이 증가하였다. …
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Direct and indirect effects of alpha-particle irradiations of human prostate tumor cells
… of DU-145 cells were studied using the clonogenic assay, the comet assay and the micronucleus assay. A novel co-culture system was then designed to study the bystander effect transmitted through the cell culture medium. The micronucleus assay was used as endpoint for the bystander …
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The effect of distinct BRCA1 splice forms on drug sensitivity in breast- and ovarian cancer cell lines
… olaparib. Drug sensitivity was studied by clonogenic assay, and treatment with olaparib demonstrated that cells with loss of BRCA1 expression are more sensitive to PARP inhibitors than cells expressing wildtype (WT) BRCA1. The clonogenic assay also revealed that cells with BRCA1 loss grew …
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Cellular response to 11[beta]-dichloro, a novel aniline mustard-estradienone, in various prostate cancer cell lines
… transcription factor hijacking hypotheses. To assay differential toxicity, five different prostate cancer cell lines were used, namely, LNCaP (AR-positive, T887A), PC3 (AR-negative, p53 inactive), DU145 (AR-negative, p53 null), and the isogenic pair PC3-AR (AR-positive) and PC3-neo …
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Fasting Reduces Intestinal Radiotoxicity Enabling Dose-Escalated Radiotherapy For Pancreatic Cancer
… analyses using the Withers-Elkind microcolony assay show that fasting protected small intestinal (SI) stem cells from radiation damage and promoted early regeneration. To show a proof-of-principle for the use of this radioporotective maneuver in cancer therapy, we used an orthotopic model of …
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Synthesis of the small peptide analogues of cyclin dependent kinase (CDK4) for cancer treatment
… synthesized by solution phase method. Biological assays were optimized. Clonogenic assay was chosen to test with our synthetic peptides against RT112 bladder cancer and MRC5-hTERT fibroblast cells
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Photofrin-Mediated Electroporation-Assisted Photodynamic Therapy of MCF-7 Human Breast Cancer and B16-F10 Mouse Melanoma Cells
… spectrophotometric intracellular concentration assays and observed a consistent and significant increase in intracellular Photofrin content in both MCF-7 and B16-F10 cell lines in those samples subjected to electroporation and an equal Photofrin concentration compared to the non-electroporated …
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Radiobiological and microdosimetric evaluation of proton FLASH versus conventional proton and conventional X-ray irradiation. A comparative study on cell survival and DNA damage
… με τη δοκιμασία κλωνογόνου επιβίωσης (clonogenic assay). Αποτελέσματα: Η μικροδοσιμετρική ανάλυση επιβεβαίωσε την μεταβαλλόμενη ποιότητα ακτινοβολίας των πρωτονίων, με το μοντέλο GSM2 να προβλέπει υψηλότερες τιμές RBE στην περιοχή αιχμής σε σύγκριση με το MKM. Στην αξιολόγηση των εστιών …
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Quantitative analysis of cellular processing of Antibody-Drug Conjugates
… level. First, we developed a flow cytometric clonogenic assay and used this assay to study the single-cell potency of the chemotherapeutic drug doxorubicin. Across a number of cancer cell lines, we found that a cell's ability to proliferate was only dependent on the amount of doxorubicin …
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Functional characterization of poly(ADP-ribose) dependent ubiquitin E3 ligase in the DNA damage response
… 16 4. HR and NHEJ analysis 17 5. Neutral comet assay 18 6. Chromatin fractionation 18 7. pADPr polymer synthesis 19 8. pADPr and SUMO overlay assay 20 9. Dot blot 20 10. In vitro ubiquitination assay 20 11. In vivo ubiquitination assay 21 12. Western blot 22 13. Mass spectrometry analysis 22 14. …
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Auger-emitter radiochemotherapy in squamous cell cancers : in vitro and in vivo experiments with In-111-BLMC
… In-111-Cl3 was studied using the 96-well plate clonogenic assay. The influence of BLM and In-111-BLMC on the cell cycle was measured with flow cytometry. In in vivo nude mice xenograft studies, the activity ratios of In-111-BLMC were obtained in gamma camera images. The effect of In-111-BLMC in …
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The development of targeting nanosystems for the treatment of glioblastoma and neuroblastoma tumours
… was investigated using the WST-1 cell viability assay at concentrations of 75-300 µg/ml for 48 hours in U87 and SH-SY5Y cells, while the non-cancerous KMST-6 human fibroblast cells served as a control cell line. The maximal inhibitory concentrations (IC₅₀ values) for all NPs in U87 and SH-SY-5Y …
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Investigation of a fusogenic pH-sensitive liposomal system for tumour-targeted intracellular delivery of dinitrobenzamide mustard prodrugs
… was investigated using a calcein self-quenching assay at pH 7.4, 6.5 and 5.5. Subsequently, SN25860 was loaded using the optimised pPSL. To increase drug loading (DL) and entrapment efficiency (EE), an active loading method (intraliposomal calcium acetate) was used; DL factors were investigated. …
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Synthesis, characterization, and anticancer activity of arene mono-ruthenium and heteroleptic mixed-valent diruthenium complexes
… towards the human breast MCF-7 cells. Clonogenic assay experiments showed that C3 inhibited the ability of MCF-7 cells to survive after 7 days of monitoring cell colony formation. Western blotting analysis suggests that C3 induces double-stranded DNA breaks, observed by the increase in …
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Viral HSV1-TK gene, radiolabeled FIAU, and ganciclovir: combined gene targeted radiotherapy and suicide gene therapy for prostate cancer
… secondly, GCV sensitivity, assessed by the MTT assay; and thirdly, by the detection of HSV1-tk gene by RT-PCR. The highest specific activity of [123I]FIAU was obtained by the use of a no-carrier added method of synthesis. The cytotoxicity of [123I]FIAU was assessed by clonogenic assay after …